malP:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

malP

Gene Synonym(s)

ECK3404, b3417, JW5689, blu, malA[1], malA

Product Desc.

maltodextrin phosphorylase[2][3];

Component of maltodextrin phosphorylase[2][3]

Maltodextrin phosphorylase[4]

Product Synonyms(s)

maltodextrin phosphorylase[1], B3417[2][1], MalA[2][1], Blu[2][1], MalP[2][1] , blu, ECK3404, JW5689, malA, b3417

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): malPQ[2], OP00049

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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MalT regulon.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

malP

Mnemonic

Maltose

Synonyms

ECK3404, b3417, JW5689, blu, malA[1], malA

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

76.47 minutes 

MG1655: 3550495..3548102
<gbrowseImage> name=NC_000913:3548102..3550495 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3481073..3478680
<gbrowseImage> name=NC_012967:3478680..3481073 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3436596..3438989
<gbrowseImage> name=NC_012759:3436596..3438989 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4087943..4090336
<gbrowseImage> name=NC_007779:4087943..4090336 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3648240..3645847
<gbrowseImage> name=NC_010473:3645847..3648240 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3548105

Edman degradation

PMID:6986282


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

malP(del) (Keio:JW5689)

deletion

deletion

PMID:16738554

Shigen

malP::Tn5KAN-I-SceI (FB21187)

Insertion at nt 452 in Plus orientation

PMID:15262929

E. coli Genome Project:FB21187

contains pKD46

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5689

Plasmid clone

Shigen

PMID:16769691

Status:cloned as old JW

Primer 1:GCCTCACAACCTATTTTTAACGA

Primer 2:CCGCGTTTTGCCTGCCAGATACG

E4E4

Kohara Phage

Genobase

PMID:3038334

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[5]

est. P1 cotransduction: 14% [6]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

glgP721::Tn10

Linked marker

CAG18638 = CGSC7451[5]

est. P1 cotransduction: 59% [6]
Synonyms:zhg-3086::Tn10, zhh-21::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10560

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10560

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000553

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947922

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0555

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011154

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MalP

Synonyms

maltodextrin phosphorylase[1], B3417[2][1], MalA[2][1], Blu[2][1], MalP[2][1] , blu, ECK3404, JW5689, malA, b3417

Product description

maltodextrin phosphorylase[2][3];

Component of maltodextrin phosphorylase[2][3]

Maltodextrin phosphorylase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0016052

carbohydrate catabolic process

P

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0000272

polysaccharide catabolic process

P

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0004645

phosphorylase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000811

F

Seeded from EcoCyc (v14.0)

complete

GO:0004645

phosphorylase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011833

F

Seeded from EcoCyc (v14.0)

complete

GO:0004645

phosphorylase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.4.1.1

F

Seeded from EcoCyc (v14.0)

complete

GO:0005975

carbohydrate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000811

P

Seeded from EcoCyc (v14.0)

complete

GO:0005975

carbohydrate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011833

P

Seeded from EcoCyc (v14.0)

complete

GO:0005975

carbohydrate metabolic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0119

P

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0016757

transferase activity, transferring glycosyl groups

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0328

F

Seeded from EcoCyc (v14.0)

complete

GO:0030170

pyridoxal phosphate binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011833

F

Seeded from EcoCyc (v14.0)

complete

GO:0031220

maltodextrin phosphorylase activity

PMID:4865311

IDA: Inferred from Direct Assay

F

complete

GO:0030980

alpha-glucan catabolic process

PMID:4865311

IDA: Inferred from Direct Assay

P

complete

GO:0005980

glycogen catabolic process

PMID:4865311

IDA: Inferred from Direct Assay

P

complete

GO:0005737

cytoplasm

PMID:4865311

IDA: Inferred from Direct Assay

C

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of maltodextrin phosphorylase

could be indirect

Protein

aceF

PMID:15690043

Experiment(s):EBI-879973, EBI-884958

Protein

lpdA

PMID:15690043

Experiment(s):EBI-879973, EBI-884958

Protein

pnp

PMID:15690043

Experiment(s):EBI-879973

Protein

rplA

PMID:15690043

Experiment(s):EBI-879973

Protein

rplC

PMID:15690043

Experiment(s):EBI-879973, EBI-884958

Protein

rplD

PMID:15690043

Experiment(s):EBI-879973

Protein

rplI

PMID:15690043

Experiment(s):EBI-884958

Protein

rplM

PMID:15690043

Experiment(s):EBI-884958

Protein

rplS

PMID:15690043

Experiment(s):EBI-884958

Protein

rplU

PMID:15690043

Experiment(s):EBI-884958

Protein

rplV

PMID:15690043

Experiment(s):EBI-884958

Protein

yhbY

PMID:15690043

Experiment(s):EBI-884958

Protein

rplA

PMID:19402753

MALDI(Z-score):24.723260

Protein

yhbY

PMID:19402753

LCMS(ID Probability):99.6

Protein

rpsP

PMID:19402753

LCMS(ID Probability):99.6

Protein

pnp

PMID:19402753

MALDI(Z-score):23.070731

Protein

rplS

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplI

PMID:19402753

LCMS(ID Probability):99.4

Protein

rplU

PMID:19402753

LCMS(ID Probability):99.6

Protein

ycjY

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplM

PMID:19402753

LCMS(ID Probability):99.6

Protein

hupA

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):28.105466

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSQPIFNDKQ FQEALSRQWQ RYGLNSAAEM TPRQWWLAVS EALAEMLRAQ PFAKPVANQR
HVNYISMEFL IGRLTGNNLL NLGWYQDVQD SLKAYDINLT DLLEEEIDPA LGNGGLGRLA
ACFLDSMATV GQSATGYGLN YQYGLFRQSF VDGKQVEAPD DWHRSNYPWF RHNEALDVQV
GIGGKVTKDG RWEPEFTITG QAWDLPVVGY RNGVAQPLRL WQATHAHPFD LTKFNDGDFL
RAEQQGINAE KLTKVLYPND NHTAGKKLRL MQQYFQCACS VADILRRHHL AGRKLHELAD
YEVIQLNDTH PTIAIPELLR VLIDEHQMSW DDAWAITSKT FAYTNHTLMP EALERWDVKL
VKGLLPRHMQ IINEINTRFK TLVEKTWPGD EKVWAKLAVV HDKQVHMANL CVVGGFAVNG
VAALHSDLVV KDLFPEYHQL WPNKFHNVTN GITPRRWIKQ CNPALAALLD KSLQKEWAND
LDQLINLEKF ADDAKFRQQY REIKQANKVR LAEFVKVRTG IEINPQAIFD IQIKRLHEYK
RQHLNLLHIL ALYKEIRENP QADRVPRVFL FGAKAAPGYY LAKNIIFAIN KVADVINNDP
LVGDKLKVVF LPDYCVSAAE KLIPAADISE QISTAGKEAS GTGNMKLALN GALTVGTLDG
ANVEIAEKVG EENIFIFGHT VEQVKAILAK GYDPVKWRKK DKVLDAVLKE LESGKYSDGD
KHAFDQMLHS IGKQGGDPYL VMADFAAYVE AQKQVDVLYR DQEAWTRAAI LNTARCGMFS
SDRSIRDYQA RIWQAKR
Length

797

Mol. Wt

90.523 kDa

pI

7.4 (calculated)

Extinction coefficient

148,740 - 149,615 (calc based on 26 Y, 20 W, and 7 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P00490

Domain

91..797

PF00343 Carbohydrate phosphorylase

PMID:19920124

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=malP taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:49176351

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947922

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011154

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P00490

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10560

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10560

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947922

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000553

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0555

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

7.52E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

8.255+/-0.068

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.088591726

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

744

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

744

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

406

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

malPQ

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3550475..3550515 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3417 (EcoliWiki Page)

NCBI GEO profiles for malP

microarray

GenExpDB:b3417 (EcoliWiki Page)

Summary of data for malP from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to malP Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10560

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0555

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3417

EcoGene

EcoGene:EG10560

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000553

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011154

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Shigella flexneri

MALP

From SHIGELLACYC

E. coli O157

MALP

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:53756

Pfam (EcoliWiki Page)

PF00343 Carbohydrate phosphorylase

EcoCyc

EcoCyc:EG10560

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10560

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000553

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0555

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011154

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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