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Expression|

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Standard Name	mak
Gene Synonym(s)	ECK0389, b0394, JW0385, yajF^[1], yajF
Product Desc.	EG11288^[2]^[3] Cryptic manno(fructo)kinase^[4]
Product Synonyms(s)	manno(fructo)kinase^[1], B0394^[2]^[1], Mak^[2]^[1], YajF^[2]^[1] , ECK0389, JW0385, yajF, b0394
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): mak^[2]
Regulation/Activity
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Notes

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Mak also has low level glucokinase activity; fructose is a much substrate than glucose in vitro. The same (probable) promoter-up mutation was isolated as rescuing both fructose (Sproul, 2001) and glucose kinase (Miller, 2005) deficiences. The original interpretation of the mak-up mutation as in the mak coding region has been clarified by identifying the GTG initiation codon at the previous codon 47, placing the mak-up mutation in the putative promoter region. The unpublished protein sequencing is noted in Miller (2005) and the actual protein sequence data obtained (MRIGIDLGGTKTEVIALGDAGEQLY) was provided as a personal communication to EcoGene from Brian Miller (March, 2006).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	mak
Mnemonic	MAnno(fructo)Kinase
Synonyms	ECK0389, b0394, JW0385, yajF^[1], yajF
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	8.82 minutes	MG1655: 409368..410276 <gbrowseImage> name=NC_000913:409368..410276 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
BW2952		NC_012759: 312127..313035 <gbrowseImage> name=NC_012759:312127..313035 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 409368..410276 <gbrowseImage> name=NC_007779:409368..410276 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 348699..349607 <gbrowseImage> name=NC_010473:348699..349607 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
Δmak (Keio:JW0385)	deletion	deletion	PMID:16738554	Shigen CGSC8561^[5]
Δmak-759::kan			PMID:16738554	CGSC:101728
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0385	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCGTATAGGTATCGATTTAGG Primer 2:CCCTCTTGTGGCCATAACCACGC
6A12	Kohara Phage	Genobase	PMID:3038334
9G9	Kohara Phage	Genobase	PMID:3038334
zai-3053::Tn10	Linked marker	CAG18091 = CGSC7336^[5]	est. P1 cotransduction: 59% ^[6] Synonyms:zaj-3053::Tn10 nnnCAG18091 also carries proC9999 (CGSC).
tsx-247::Tn10	Linked marker	CAG12148 = CGSC7337^[5]	est. P1 cotransduction: 44% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11288	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11288	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001261	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:949086	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1265	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001372	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Mak
Synonyms	manno(fructo)kinase^[1], B0394^[2]^[1], Mak^[2]^[1], YajF^[2]^[1] , ECK0389, JW0385, yajF, b0394
Product description	EG11288^[2]^[3] Cryptic manno(fructo)kinase^[4]
EC number (for enzymes)	2.7.1.4^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005975	carbohydrate metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0119	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008865	fructokinase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.7.1.4	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016301	kinase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0418	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	nlpE	PMID:16606699	Experiment(s):EBI-1136448
Protein	groL	PMID:16606699	Experiment(s):EBI-1136448
Protein	rplI	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplA	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplR	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplO	PMID:19402753	LCMS(ID Probability):99.6
Protein	eno	PMID:19402753	LCMS(ID Probability):99.6
Protein	rpsM	PMID:19402753	LCMS(ID Probability):99.6
Protein	rpmC	PMID:19402753	LCMS(ID Probability):99.6
Protein	rpsT	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplY	PMID:19402753	LCMS(ID Probability):99.6
Protein	ahpC	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplX	PMID:19402753	LCMS(ID Probability):99.6
Protein	groS	PMID:19402753	LCMS(ID Probability):99.6
Protein	hdeB	PMID:19402753	LCMS(ID Probability):99.6
Protein	hupA	PMID:19402753	LCMS(ID Probability):99.6
Protein	hns	PMID:19402753	LCMS(ID Probability):99.6
Protein	pflB	PMID:19402753	LCMS(ID Probability):99.6
Protein	dps	PMID:19402753	LCMS(ID Probability):99.6
Protein	yfiD	PMID:19402753	LCMS(ID Probability):99.6
Protein	slyD	PMID:19402753	LCMS(ID Probability):99.6
Protein	tig	PMID:19402753	LCMS(ID Probability):99.6
Protein	yajQ	PMID:19402753	LCMS(ID Probability):99.6
Protein	yfcZ	PMID:19402753	LCMS(ID Probability):99.4
Protein	zapB	PMID:19402753	LCMS(ID Probability):99.6
Protein	pgm	PMID:19402753	LCMS(ID Probability):99.6
Protein	queA	PMID:19402753	MALDI(Z-score):37.839387
Protein	talB	PMID:19402753	LCMS(ID Probability):99.6
Protein	ppsA	PMID:19402753	LCMS(ID Probability):99.6
Protein	yjbJ	PMID:19402753	LCMS(ID Probability):99.6
Protein	ldhA	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MNHSMIISTA LSLNLAGMIT NCRRPCIANP VVRLYAIDIE KNKESTVRIG IDLGGTKTEV IALGDAGEQL YRHRLPTPRD DYRQTIETIA TLVDMAEQAT GQRGTVGMGI PGSISPYTGV VKNANSTWLN GQPFDKDLSA RLQREVRLAN DANCLAVSEA VDGAAAGAQT VFAVIIGTGC GAGVAFNGRA HIGGNGTAGE WGHNPLPWMD EDELRYREEV PCYCGKQGCI ETFISGTGFA MDYRRLSGHA LKGSEIIRLV EESDPVAELA LRRYELRLAK SLAHVVNILD PDVIVLGGGM SNVDRLYQTV GQLIKQFVFG GECETPVRKA KHGDSSGVRG AAWLWPQE
Length	348
Mol. Wt	37.553 kDa
pI	6.2 (calculated)
Extinction coefficient	40,910 - 41,910 (calc based on 9 Y, 5 W, and 8 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	4..193	PF00480 ROK family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mak taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111128	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:949086	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001372	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P23917	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11288	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11288	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:949086	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001261	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1265	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	1.86E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	458	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	279	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	368	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
mak
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:409348..409388 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0394 (EcoliWiki Page)	NCBI GEO profiles for mak
microarray	GenExpDB:b0394 (EcoliWiki Page)	Summary of data for mak from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to mak Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11288	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1265	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0394
EcoGene	EcoGene:EG11288	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001261	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001372	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	YAJF	From SHIGELLACYC
E. coli O157	YAJF	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Panther (EcoliWiki Page)	PTHR18964:SF13
Superfamily (EcoliWiki Page)	SUPERFAMILY:53067
Pfam (EcoliWiki Page)	PF00480 ROK family
EcoCyc	EcoCyc:EG11288	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11288	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001261	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1265	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001372	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

mak:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to mak Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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