maeA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

maeA

Gene Synonym(s)

sfcA, ECK1473, b1479, JW5238, mae, maeA?[1][2][3]

Product Desc.

MaeA[3]; malate dehydrogenase, NAD-requiring[2];

Component of malic enzyme, NAD-linked[3]; malate dehydrogenase, NAD-requiring[2]

Malic enzyme, NAD-dependent; NAD-ME[4]

Product Synonyms(s)

malate dehydrogenase, (decarboxylating, NAD-requiring) (malic enzyme)[1], B1479[3][1], MaeA[3][1], SfcA[3][1] , ECK1473, JW5238, sfcA, b1479

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): sfcA[3]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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MaeA catalyzes four reactions: I, NAD-dependent L-malate decarboxylation; II, NAD-independent oxalacetate decarboxylation; III, oxalacetate reductase, NAD-dependent; !V, pyruvate reductase, NAD-dependent. Acetyl-CoA and ATP are allosteric inhibitors; aspartate is an allosteric activator. Reaction I proceeds by two steps: dehydrogenation, then decarboxylation.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

maeA

Mnemonic

Malic enzyme

Synonyms

sfcA, ECK1473, b1479, JW5238, mae, maeA?[1][2][3]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

33.45 minutes 

MG1655: 1553693..1551996
<gbrowseImage> name=NC_000913:1551996..1553693 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1527910..1529607
<gbrowseImage> name=NC_012967:1527910..1529607 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1444055..1445752
<gbrowseImage> name=NC_012759:1444055..1445752 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1557383..1555686
<gbrowseImage> name=NC_007779:1555686..1557383 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1644288..1642591
<gbrowseImage> name=NC_010473:1642591..1644288 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

sfcA::Tn5KAN-2 (FB20333)

Insertion at nt 801 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20333

contains pKD46

sfcA::Tn5KAN-2 (FB20334)

Insertion at nt 801 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20334

does not contain pKD46

ΔsfcA::kan

deletion

Biolog:respiration

unable to respire D-Mannose

PMID:16095938

ΔsfcA772::kan

PMID:16738554

CGSC:102924


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691

Status:

Primer 1:

Primer 2:

1G9

Kohara Phage

Genobase

PMID:3038334

9B8

Kohara Phage

Genobase

PMID:3038334

10C7

Kohara Phage

Genobase

PMID:3038334

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10948

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10948

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000937

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946031

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0941

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004931

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

SfcA

Synonyms

malate dehydrogenase, (decarboxylating, NAD-requiring) (malic enzyme)[1], B1479[3][1], MaeA[3][1], SfcA[3][1] , ECK1473, JW5238, sfcA, b1479

Product description

MaeA[3]; malate dehydrogenase, NAD-requiring[2];

Component of malic enzyme, NAD-linked[3]; malate dehydrogenase, NAD-requiring[2]

Malic enzyme, NAD-dependent; NAD-ME[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0005488

binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc (v14.0)

complete

GO:0006108

malate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001891

P

Seeded from EcoCyc (v14.0)

complete

GO:0016619

malate dehydrogenase (oxaloacetate-decarboxylating) activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01619

F

Seeded from EcoCyc (v14.0)

complete

GO:0016619

malate dehydrogenase (oxaloacetate-decarboxylating) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.1.1.38

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015884

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0051287

NAD or NADH binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012302

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015884

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of malic enzyme, NAD-linked

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MDIQKRVSDM EPKTKKQRSL YIPYAGPVLL EFPLLNKGSA FSMEERRNFN LLGLLPEVVE
TIEEQAERAW IQYQGFKTEI DKHIYLRNIQ DTNETLFYRL VNNHLDEMMP VIYTPTVGAA
CERFSEIYRR SRGVFISYQN RHNMDDILQN VPNHNIKVIV VTDGERILGL GDQGIGGMGI
PIGKLSLYTA CGGISPAYTL PVVLDVGTNN QQLLNDPLYM GWRNPRITDD EYYEFVDEFI
QAVKQRWPDV LLQFEDFAQK NAMPLLNRYR NEICSFNDDI QGTAAVTVGT LIAASRAAGG
QLSEKKIVFL GAGSAGCGIA EMIISQTQRE GLSEEAARQK VFMVDRFGLL TDKMPNLLPF
QTKLVQKREN LSDWDTDSDV LSLLDVVRNV KPDILIGVSG QTGLFTEEII REMHKHCPRP
IVMPLSNPTS RVEATPQDII AWTEGNALVA TGSPFNPVVW KDKIYPIAQC NNAFIFPGIG
LGVIASGASR ITDEMLMSAS ETLAQYSPLV LNGEGMVLPE LKDIQKVSRA IAFAVGKMAQ
QQGVAVKTSA EALQQAIDDN FWQAEYRDYR RTSI
Length

574

Mol. Wt

64.271 kDa

pI

5.1 (calculated)

Extinction coefficient

65,320 - 66,070 (calc based on 18 Y, 7 W, and 6 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

81..261

PF00390 Malic enzyme, N-terminal domain

PMID:19920124

Domain

271..531

PF03949 Malic enzyme, NAD binding domain

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=maeA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111281

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946031

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004931

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P26616

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10948

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10948

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946031

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000937

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0941

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

5.30E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

1331

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

861

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1143

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

sfcA

File:.jpg

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1553673..1553713 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1479 (EcoliWiki Page)

NCBI GEO profiles for maeA

microarray

GenExpDB:b1479 (EcoliWiki Page)

Summary of data for maeA from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to maeA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10948

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0941

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1479

EcoGene

EcoGene:EG10948

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000937

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004931

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000011712 (score: 1.000; bootstrap: 100%)
  • ENSANGP00000019421 (score: 0.337)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000001599 (score: 1.000; bootstrap: 100%)
  • ENSAPMP00000016844 (score: 0.398)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT1G79750 (score: 1.000; bootstrap: 91%)
  • AT5G11670 (score: 0.571)
  • AT2G19900 (score: 0.566)
  • AT5G25880 (score: 0.564)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000000306 (score: 1.000; bootstrap: 100%)
  • ENSBTAP00000021641 (score: 0.120)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00012983 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000006606 (score: 1.000; bootstrap: 100%)
  • ENSCAFP00000000245 (score: 1.000; bootstrap: 100%)
  • ENSCAFP00000004362 (score: 0.580)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-040801-147 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-040801-158 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0191268 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0029155 (score: 1.000; bootstrap: 99%)
  • FBgn0002719 (score: 0.266)
  • FBgn0029154 (score: 0.103)
  • FBgn0029153 (score: 0.098)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA19206-PA (score: 1.000; bootstrap: 99%)
  • GA10087-PA (score: 0.224)
  • GA15647-PA (score: 0.114)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000025506 (score: 1.000; bootstrap: 100%)
  • ENSGALP00000027827 (score: 0.434)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000265285 (score: 1.000; bootstrap: 100%)
  • ENSP00000352657 (score: 0.585)
  • ENSP00000321070 (score: 0.306)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000028977 (score: 1.000; bootstrap: 89%)
  • ENSMMUP00000017153 (score: 0.256)
  • ENSMMUP00000016529 (score: 0.244)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000022921 (score: 1.000; bootstrap: 100%)
  • ENSMODP00000016545 (score: 0.832)
  • ENSMODP00000005123 (score: 0.619)
  • ENSMODP00000013725 (score: 0.337)

From Inparanoid:20070104

Mus musculus

  • MGI:97043 (score: 1.000; bootstrap: 100%)
  • MGI:1916679 (score: 0.574)
  • MGI:2147351 (score: 0.310)

From Inparanoid:20070104

Oryza gramene

  • P43279 (score: 1.000; bootstrap: 88%)
  • Q9LDH7 (score: 0.983)
  • Q8W000 (score: 0.775)
  • Q9ZRH5 (score: 0.749)
  • Q84LQ5 (score: 0.714)
  • P16243 (score: 0.711)
  • Q84LP6 (score: 0.708)
  • Q6T5D1 (score: 0.666)
  • Q9FRT2 (score: 0.625)
  • Q6SZS7 (score: 0.561)
  • Q5JKW5 (score: 0.561)
  • O50015 (score: 0.551)
  • Q5G1U0 (score: 0.545)
  • Q5JNA0 (score: 0.506)
  • Q5JN99 (score: 0.265)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000031411 (score: 1.000; bootstrap: 100%)
  • ENSPTRP00000007130 (score: 0.547)
  • ENSPTRP00000017045 (score: 0.303)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000013244 (score: 1.000; bootstrap: 100%)
  • ENSRNOP00000032974 (score: 0.496)
  • ENSRNOP00000020918 (score: 0.308)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YKL029C (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPCC7942c (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000154391 (score: 1.000; bootstrap: 100%)
  • NEWSINFRUP00000138074 (score: 0.497)
  • NEWSINFRUP00000154894 (score: 0.211)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00032637001 (score: 1.000; bootstrap: 100%)
  • GSTENP00013207001 (score: 0.249)
  • GSTENP00025974001 (score: 0.172)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000049052 (score: 1.000; bootstrap: 100%)
  • ENSXETP00000022903 (score: 0.685)
  • ENSXETP00000048456 (score: 0.326)

From Inparanoid:20070104

Shigella flexneri

SFCA

From SHIGELLACYC

E. coli O157

SFCA

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF03949 Malic enzyme, NAD binding domain

Pfam (EcoliWiki Page)

PF00390 Malic enzyme, N-terminal domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:51735

Panther (EcoliWiki Page)

PTHR23406:SF2

Superfamily (EcoliWiki Page)

SUPERFAMILY:53223

EcoCyc

EcoCyc:EG10948

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10948

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000937

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0941

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004931

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.

Categories

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