maeA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

SfcA

Synonyms

malate dehydrogenase, (decarboxylating, NAD-requiring) (malic enzyme)[1], B1479[2][1], MaeA[2][1], SfcA[2][1] , ECK1473, JW5238, sfcA, b1479

Product description

MaeA[2]; malate dehydrogenase, NAD-requiring[3];

Component of malic enzyme, NAD-linked[2]; malate dehydrogenase, NAD-requiring[3]

Malic enzyme, NAD-dependent; NAD-ME[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0005488

binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc (v14.0)

complete

GO:0006108

malate metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001891

P

Seeded from EcoCyc (v14.0)

complete

GO:0016619

malate dehydrogenase (oxaloacetate-decarboxylating) activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01619

F

Seeded from EcoCyc (v14.0)

complete

GO:0016619

malate dehydrogenase (oxaloacetate-decarboxylating) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.1.1.38

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015884

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0051287

NAD or NADH binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012302

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015884

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of malic enzyme, NAD-linked

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MDIQKRVSDM EPKTKKQRSL YIPYAGPVLL EFPLLNKGSA FSMEERRNFN LLGLLPEVVE
TIEEQAERAW IQYQGFKTEI DKHIYLRNIQ DTNETLFYRL VNNHLDEMMP VIYTPTVGAA
CERFSEIYRR SRGVFISYQN RHNMDDILQN VPNHNIKVIV VTDGERILGL GDQGIGGMGI
PIGKLSLYTA CGGISPAYTL PVVLDVGTNN QQLLNDPLYM GWRNPRITDD EYYEFVDEFI
QAVKQRWPDV LLQFEDFAQK NAMPLLNRYR NEICSFNDDI QGTAAVTVGT LIAASRAAGG
QLSEKKIVFL GAGSAGCGIA EMIISQTQRE GLSEEAARQK VFMVDRFGLL TDKMPNLLPF
QTKLVQKREN LSDWDTDSDV LSLLDVVRNV KPDILIGVSG QTGLFTEEII REMHKHCPRP
IVMPLSNPTS RVEATPQDII AWTEGNALVA TGSPFNPVVW KDKIYPIAQC NNAFIFPGIG
LGVIASGASR ITDEMLMSAS ETLAQYSPLV LNGEGMVLPE LKDIQKVSRA IAFAVGKMAQ
QQGVAVKTSA EALQQAIDDN FWQAEYRDYR RTSI
Length

574

Mol. Wt

64.271 kDa

pI

5.1 (calculated)

Extinction coefficient

65,320 - 66,070 (calc based on 18 Y, 7 W, and 6 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

81..261

PF00390 Malic enzyme, N-terminal domain

PMID:19920124[5]

Domain

271..531

PF03949 Malic enzyme, NAD binding domain

PMID:19920124[5]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=maeA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111281

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946031

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004931

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P26616

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10948

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10948

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946031

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000937

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0941

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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