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Standard Name	macA
Gene Synonym(s)	ECK0869, b0878, JW0862, ybjY^[1], ybjY
Product Desc.	MacA^[2]^[3]; Component of MacAB macrolide efflux transporter complex^[2]; MacAB-TolC Macrolide Efflux Transport System^[3] Membrane fusion protein (MFP) for macrolide efflux; subunit of ABC-type, TolC-dependent transporter MacAB; signal anchor; periplasmic^[4]
Product Synonyms(s)	macrolide transporter subunit, membrane fusion protein (MFP) component^[1], B0878^[2]^[1], YbjY^[2]^[1], MacA^[2]^[1] , ECK0869, JW0862, ybjY, b0878
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s):
Regulation/Activity
Quick Links
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Notes

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OmpF consistently co-purifies with MacA; MacA stimulates MacB ATPase activity (Tikhonova, 2007).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	macA
Mnemonic	Macrolide-specific ABC-type efflux carrier
Synonyms	ECK0869, b0878, JW0862, ybjY^[1], ybjY
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	19.8 minutes	MG1655: 918458..919573 <gbrowseImage> name=NC_000913:918458..919573 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 935941..937056 <gbrowseImage> name=NC_012967:935941..937056 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 821426..822541 <gbrowseImage> name=NC_012759:821426..822541 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 919657..920772 <gbrowseImage> name=NC_007779:919657..920772 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 972386..973501 <gbrowseImage> name=NC_010473:972386..973501 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	918458	Edman degradation	PMID:17214741
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔmacA (Keio:JW0862)	deletion	deletion	PMID:16738554	Shigen CGSC8894^[5]
ΔmacA779::kan			PMID:16738554	CGSC:101722
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0862	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAAAAGCGGAAAACCGTGAA Primer 2:CCTTGTGCAGCTCCTGGTTTGGC
1E5	Kohara Phage	Genobase	PMID:3038334
1H1	Kohara Phage	Genobase	PMID:3038334
zbh-29::Tn10	Linked marker	CAG18493 = CGSC7353^[5]	est. P1 cotransduction: % ^[6] Synonyms:zbi-29::Tn10
zca-1230::Tn10	Linked marker	CAG18478 = CGSC7356^[5]	est. P1 cotransduction: 34% ^[6] Synonyms:zbj-1230::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6461	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13694	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003061	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947322	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3458	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002986	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	MacA
Synonyms	macrolide transporter subunit, membrane fusion protein (MFP) component^[1], B0878^[2]^[1], YbjY^[2]^[1], MacA^[2]^[1] , ECK0869, JW0862, ybjY, b0878
Product description	MacA^[2]^[3]; Component of MacAB macrolide efflux transporter complex^[2]; MacAB-TolC Macrolide Efflux Transport System^[3] Membrane fusion protein (MFP) for macrolide efflux; subunit of ABC-type, TolC-dependent transporter MacAB; signal anchor; periplasmic^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008565	protein transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006143	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009306	protein secretion	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006143	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006143	C	Seeded from EcoCyc (v14.0)	complete
	GO:0019898	extrinsic to membrane	PMID:11544226	IDA: Inferred from Direct Assay		C		complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0019898	extrinsic to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9903	C	Seeded from EcoCyc (v14.0)	complete
	GO:0046677	response to antibiotic	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0046	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of MacAB macrolide efflux transporter complex	could be indirect
Protein	rplV		PMID:16606699	Experiment(s):EBI-1138239
Protein	rpmB		PMID:16606699	Experiment(s):EBI-1138239
Protein	yibL		PMID:16606699	Experiment(s):EBI-1138239
Protein	atpD		PMID:19402753	MALDI(Z-score):22.953635
Protein	uidA		PMID:19402753	LCMS(ID Probability):98.8
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MAYGVFRKFM KKRKTVKKRY VIALVIVIAG LITLWRILNA PVPTYQTLIV RPGDLQQSVL ATGKLDALRK VDVGAQVSGQ LKTLSVAIGD KVKKDQLLGV IDPEQAENQI KEVEATLMEL RAQRQQAEAE LKLARVTYSR QQRLAQTKAV SQQDLDTAAT EMAVKQAQIG TIDAQIKRNQ ASLDTAKTNL DYTRIVAPMA GEVTQITTLQ GQTVIAAQQA PNILTLADMS AMLVKAQVSE ADVIHLKPGQ KAWFTVLGDP LTRYEGQIKD VLPTPEKVND AIFYYARFEV PNPNGLLRLD MTAQVHIQLT DVKNVLTIPL SALGDPVGDN RYKVKLLRNG ETREREVTIG ARNDTDVEIV KGLEAGDEVV IGEAKPGAAQ
Length	380
Mol. Wt	41.724 kDa
pI	9.6 (calculated)
Extinction coefficient	24,410 (calc based on 9 Y, 2 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-31	UniProt Manual:Signal Peptides	UniProt:P75830
Domain	60..238	PF00529 HlyD family secretion protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=macA taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111182	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947322	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002986	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P75830	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6461	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13694	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947322	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003061	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3458	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	224	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	134	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	180	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:918438..918478 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0878 (EcoliWiki Page)	NCBI GEO profiles for macA
microarray	GenExpDB:b0878 (EcoliWiki Page)	Summary of data for macA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (918269..918459) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ02; Well:F12^[7]
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Notes

Accessions Related to macA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6461	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3458	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0878
EcoGene	EcoGene:EG13694	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003061	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002986	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000000826 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	S0878	From SHIGELLACYC
E. coli O157	Z1115	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00529 HlyD family secretion protein
Superfamily (EcoliWiki Page)	SUPERFAMILY:111369
EcoCyc	EcoCyc:G6461	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13694	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003061	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3458	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0002986	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 ^2.9 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

macA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to macA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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