lysU:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

lysU

Gene Synonym(s)

ECK4123, b4129, JW4090, supN[1]

Product Desc.

lysyl-tRNA synthetase (LysRSu), inducible[2]; lysyl-tRNA synthetase[3];

Component of lysyl-tRNA synthetase (LysRSu), inducible[2]

Lysine--tRNA ligase, heat inducible; Ap4A and Ap3A synthase[4]

Product Synonyms(s)

lysine tRNA synthetase, inducible[1], B4129[2][1], LysU[2][1] , ECK4123, JW4090, b4129

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): lysU[2], OP00312

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lysU

Mnemonic

Lysine

Synonyms

ECK4123, b4129, JW4090, supN[1]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

93.78 minutes 

MG1655: 4352740..4351223
<gbrowseImage> name=NC_000913:4351223..4352740 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4333437..4331920
<gbrowseImage> name=NC_012967:4331920..4333437 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4289958..4291475
<gbrowseImage> name=NC_012759:4289958..4291475 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4359395..4357878
<gbrowseImage> name=NC_007779:4357878..4359395 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4453102..4451585
<gbrowseImage> name=NC_010473:4451585..4453102 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4351226

Edman degradation

PMID:2183178


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔlysU (Keio:JW4090)

deletion

deletion

PMID:16738554

Shigen
CGSC10946[5]

ΔlysU756::kan

PMID:16738554

CGSC:101716


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Notes

Genetic Interactions

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<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4090

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTCTGAACAAGAAACACGGGG

Primer 2:CCTTTCTGTGGGCGCATCGCCGG

5H2

Kohara Phage

Genobase

PMID:3038334

21H11

Kohara Phage

Genobase

PMID:3038334

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[5]

est. P1 cotransduction: 13% [6]

cadB2231::Tn10

Linked marker

CAG18488 = CGSC7479[5]

est. P1 cotransduction: 83% [6]
Synonyms:zjd-2231::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10553

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10553

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000546

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948645

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0548

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013520

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

LysU

Synonyms

lysine tRNA synthetase, inducible[1], B4129[2][1], LysU[2][1] , ECK4123, JW4090, b4129

Product description

lysyl-tRNA synthetase (LysRSu), inducible[2]; lysyl-tRNA synthetase[3];

Component of lysyl-tRNA synthetase (LysRSu), inducible[2]

Lysine--tRNA ligase, heat inducible; Ap4A and Ap3A synthase[4]

EC number (for enzymes)

6.1.1.6[1]

Aminoacyl tRNA biosynthetic pathway & Lysine Biosynthetic pathway

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002313

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004364

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006195

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018149

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018150

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00252

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc (v14.0)

complete

GO:0016874

ligase activity

PMID:7782306

IDA: Inferred from Direct Assay

F

complete

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004365

F

Seeded from EcoCyc (v14.0)

complete

GO:0004824

lysine-tRNA ligase activity

PMID:7782306

IGI: Inferred from Genetic Interaction

EcoliWiki:lysS

F

complete

GO:0004824

lysine-tRNA ligase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00252

F

Seeded from EcoCyc (v14.0)

complete

GO:0006418

tRNA aminoacylation for protein translation

PMID:7782306

IGI: Inferred from Genetic Interaction

EcoliWiki:lysS

P

complete

GO:0004824

lysine-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002313

F

Seeded from EcoCyc (v14.0)

complete

GO:0006430

lysyl-tRNA aminoacylation

PMID:7782306

IGI: Inferred from Genetic Interaction

EcoliWiki:lysS

P

complete

GO:0004824

lysine-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018149

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

PMID:10913247

IDA: Inferred from Direct Assay

F

complete

GO:0004824

lysine-tRNA ligase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:6.1.1.6

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

PMID:10913247

IDA: Inferred from Direct Assay

F

complete

GO:0004824

lysine-tRNA ligase activity

PMID:7782306

IGI: Inferred from Genetic Interaction

F

Seeded from EcoCyc (v14.0)

Missing: with/from

GO:0006412

translation

PMID:8521806

IGI: Inferred from Genetic Interaction

EcoliWiki:translation

P

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00252

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002313

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004364

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:3325036

IC: Inferred by Curator

C

Missing: with/from

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006195

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018149

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018150

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002313

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004364

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006195

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018149

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018150

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0648

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

PMID:8521806

IGI: Inferred from Genetic Interaction

P

Seeded from EcoCyc (v14.0)

Missing: with/from

GO:0006430

lysyl-tRNA aminoacylation

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00252

P

Seeded from EcoCyc (v14.0)

complete

GO:0006430

lysyl-tRNA aminoacylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002313

P

Seeded from EcoCyc (v14.0)

complete

GO:0006430

lysyl-tRNA aminoacylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018149

P

Seeded from EcoCyc (v14.0)

complete

GO:0006430

lysyl-tRNA aminoacylation

PMID:7782306

IGI: Inferred from Genetic Interaction

P

Seeded from EcoCyc (v14.0)

Missing: with/from

GO:0016020

membrane

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of lysyl-tRNA synthetase (LysRSu), inducible

could be indirect

Protein

aceE

PMID:15690043

Experiment(s):EBI-890346

Protein

adhE

PMID:15690043

Experiment(s):EBI-890346

Protein

clpB

PMID:15690043

Experiment(s):EBI-890346

Protein

dnaN

PMID:15690043

Experiment(s):EBI-890346

Protein

dps

PMID:15690043

Experiment(s):EBI-890346, EBI-895275

Protein

fusA

PMID:15690043

Experiment(s):EBI-890346

Protein

gadA

PMID:15690043

Experiment(s):EBI-890346

Protein

gadB

PMID:15690043

Experiment(s):EBI-890346

Protein

gapA

PMID:15690043

Experiment(s):EBI-890346

Protein

katE

PMID:15690043

Experiment(s):EBI-890346

Protein

lysA

PMID:15690043

Experiment(s):EBI-890346

Protein

groL

PMID:15690043

Experiment(s):EBI-890346

Protein

pflB

PMID:15690043

Experiment(s):EBI-890346, EBI-895275

Protein

pgk

PMID:15690043

Experiment(s):EBI-890346

Protein

rplB

PMID:15690043

Experiment(s):EBI-890346

Protein

rpoB

PMID:15690043

Experiment(s):EBI-890346

Protein

rpoC

PMID:15690043

Experiment(s):EBI-890346

Protein

rpsB

PMID:15690043

Experiment(s):EBI-890346, EBI-895275

Protein

rpsK

PMID:15690043

Experiment(s):EBI-890346

Protein

tnaA

PMID:15690043

Experiment(s):EBI-890346

Protein

tufA

PMID:15690043

Experiment(s):EBI-890346, EBI-895275

Protein

eno

PMID:15690043

Experiment(s):EBI-890346, EBI-895275

Protein

rplB

PMID:16606699

Experiment(s):EBI-1147510

Protein

ileS

PMID:16606699

Experiment(s):EBI-1147510

Protein

yehI

PMID:16606699

Experiment(s):EBI-1147510

Protein

ydhJ

PMID:15690043

Experiment(s):EBI-895275

Protein

yfcZ

PMID:15690043

Experiment(s):EBI-895275

Protein

cspC

PMID:15690043

Experiment(s):EBI-895275

Protein

cysK

PMID:15690043

Experiment(s):EBI-895275

Protein

focB

PMID:15690043

Experiment(s):EBI-895275

Protein

rplI

PMID:15690043

Experiment(s):EBI-895275

Protein

rplX

PMID:15690043

Experiment(s):EBI-895275

Protein

rplY

PMID:15690043

Experiment(s):EBI-895275

Protein

rpsC

PMID:15690043

Experiment(s):EBI-895275

Protein

rpsG

PMID:15690043

Experiment(s):EBI-895275

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-895275

Protein

serS

PMID:15690043

Experiment(s):EBI-895275

Protein

wcaI

PMID:15690043

Experiment(s):EBI-895275

Protein

wrbA

PMID:15690043

Experiment(s):EBI-895275

Protein

yagJ

PMID:15690043

Experiment(s):EBI-895275

Protein

ygiW

PMID:15690043

Experiment(s):EBI-895275

Protein

nlpI

PMID:15690043

Experiment(s):EBI-895275

Protein

yhjX

PMID:15690043

Experiment(s):EBI-895275

Protein

dnaN

PMID:19402753

MALDI(Z-score):25.942724

Protein

eno

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

Protein

rfaD

PMID:19402753

MALDI(Z-score):17.908007

Protein

gadB

PMID:19402753

MALDI(Z-score):17.900703

Protein

dps

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):39.268839

Protein

serS

PMID:19402753

LCMS(ID Probability):99.0

Protein

clpB

PMID:19402753

MALDI(Z-score):17.053160

Protein

gapA

PMID:19402753

MALDI(Z-score):20.539819

Protein

yfiD

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):25.080743

Protein

pflB

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

Protein

rplY

PMID:19402753

LCMS(ID Probability):99.0

Protein

pgk

PMID:19402753

MALDI(Z-score):17.053160

Protein

adhE

PMID:19402753

MALDI(Z-score):23.622405

Protein

tnaA

PMID:19402753

MALDI(Z-score):24.637611

Protein

yfcZ

PMID:19402753

LCMS(ID Probability):99.0

Protein

cysK

PMID:19402753

LCMS(ID Probability):99.0

Protein

wrbA

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):7.204307

Protein

nlpI

PMID:19402753

LCMS(ID Probability):99.0

Protein

yhjX

PMID:19402753

LCMS(ID Probability):99.0

Protein

focB

PMID:19402753

LCMS(ID Probability):99.0

Protein

ydhJ

PMID:19402753

LCMS(ID Probability):99.0

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</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSEQETRGAN EAIDFNDELR NRREKLAALR QQGVAFPNDF RRDHTSDQLH EEFDAKDNQE
LESLNIEVSV AGRMMTRRIM GKASFVTLQD VGGRIQLYVA RDSLPEGVYN DQFKKWDLGD
IIGARGTLFK TQTGELSIHC TELRLLTKAL RPLPDKFHGL QDQEVRYRQR YLDLIANDKS
RQTFVVRSKI LAAIRQFMVA RGFMEVETPM MQVIPGGASA RPFITHHNAL DLDMYLRIAP
ELYLKRLVVG GFERVFEINR NFRNEGISVR HNPEFTMMEL YMAYADYHDL IELTESLFRT
LAQEVLGTTK VTYGEHVFDF GKPFEKLTMR EAIKKYRPET DMADLDNFDA AKALAESIGI
TVEKSWGLGR IVTEIFDEVA EAHLIQPTFI TEYPAEVSPL ARRNDVNPEI TDRFEFFIGG
REIGNGFSEL NDAEDQAERF QEQVNAKAAG DDEAMFYDED YVTALEYGLP PTAGLGIGID
RMIMLFTNSH TIRDVILFPA MRPQK
Length

505

Mol. Wt

57.828 kDa

pI

5.0 (calculated)

Extinction coefficient

33,350 - 33,475 (calc based on 15 Y, 2 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A8N5

Modification Site

133

phosphorylation site at T133

probability greater than 75%

PMID:17938405

Domain

68..146

PF01336 OB-fold nucleic acid binding domain

PMID:19920124

Domain

162..503

PF00152 tRNA synthetases class II (D, K and N)

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lysU taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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<protect></protect>

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131955

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948645

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013520

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A8N5

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10553

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10553

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948645

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000546

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0548

Escherichia coli str. K-12 substr. MG1655

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<protect></protect>

Notes

Links

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Expression

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{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

6.58E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

26.853+/-0.403

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.020435069

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

1644

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

315

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

768

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

lysU

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4352720..4352760 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b4129 (EcoliWiki Page)

NCBI GEO profiles for lysU

microarray

GenExpDB:b4129 (EcoliWiki Page)

Summary of data for lysU from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (4352207..4352578) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ10; Well:C1[7]

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Notes

Accessions Related to lysU Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10553

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0548

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4129

EcoGene

EcoGene:EG10553

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000546

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013520

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000018541 (score: 1.000; bootstrap: 100%)
  • ENSANGP00000015585 (score: 0.625)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000017005 (score: 1.000; bootstrap: 60%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT3G13490 (score: 1.000; bootstrap: 88%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000020828 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00042783 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00002238 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000029741 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000019375 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-021115-8 (score: 1.000; bootstrap: 100%)
  • ZDB-CDNA-040718-103 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0231263 (score: 1.000; bootstrap: 100%)
  • DDB0231264 (score: 0.057)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0027084 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA11433-PA (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000030796 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000325448 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000007599 (score: 1.000; bootstrap: 100%)
  • ENSMMUP00000003387 (score: 0.264)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000002697 (score: 1.000; bootstrap: 100%)
  • ENSMODP00000002726 (score: 0.796)

From Inparanoid:20070104

Mus musculus

  • MGI:1934754 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Oryza gramene

  • Q6K1T2 (score: 1.000; bootstrap: 87%)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000014280 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000044454 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YDR037W (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPBC17G93c (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000141816 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00017760001 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000004655 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

E. coli O157

LYSU

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01336 OB-fold nucleic acid binding domain

Superfamily (EcoliWiki Page)

SUPERFAMILY:50249

Superfamily (EcoliWiki Page)

SUPERFAMILY:55681

Pfam (EcoliWiki Page)

PF00152 tRNA synthetases class II (D, K and N)

EcoCyc

EcoCyc:EG10553

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10553

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000546

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0548

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013520

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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