lysU:Gene Product(s)
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
LysU |
|---|---|
| Synonyms |
lysine tRNA synthetase, inducible[1], B4129[2][1], LysU[2][1] , ECK4123, JW4090, b4129 |
| Product description |
lysyl-tRNA synthetase (LysRSu), inducible[2]; lysyl-tRNA synthetase[3]; Component of lysyl-tRNA synthetase (LysRSu), inducible[2] Lysine--tRNA ligase, heat inducible; Ap4A and Ap3A synthase[4] |
| EC number (for enzymes) |
Aminoacyl tRNA biosynthetic pathway & Lysine Biosynthetic pathway |
| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0000166 |
nucleotide binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000166 |
nucleotide binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000166 |
nucleotide binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000166 |
nucleotide binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000166 |
nucleotide binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000287 |
magnesium ion binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000287 |
magnesium ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0016874 |
ligase activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0003676 |
nucleic acid binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0004824 |
lysine-tRNA ligase activity |
IGI: Inferred from Genetic Interaction |
EcoliWiki:lysS |
F |
complete | |||
| GO:0004824 |
lysine-tRNA ligase activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006418 |
tRNA aminoacylation for protein translation |
IGI: Inferred from Genetic Interaction |
EcoliWiki:lysS |
P |
complete | |||
| GO:0004824 |
lysine-tRNA ligase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006430 |
lysyl-tRNA aminoacylation |
IGI: Inferred from Genetic Interaction |
EcoliWiki:lysS |
P |
complete | |||
| GO:0004824 |
lysine-tRNA ligase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0000287 |
magnesium ion binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0004824 |
lysine-tRNA ligase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0004824 |
lysine-tRNA ligase activity |
IGI: Inferred from Genetic Interaction |
F |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0006412 |
translation |
IGI: Inferred from Genetic Interaction |
EcoliWiki:translation |
P |
complete | |||
| GO:0005524 |
ATP binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
IC: Inferred by Curator |
C |
Missing: with/from | ||||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005829 |
cytosol |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0006412 |
translation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006412 |
translation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006412 |
translation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006412 |
translation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006412 |
translation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006412 |
translation |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006412 |
translation |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0006430 |
lysyl-tRNA aminoacylation |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006430 |
lysyl-tRNA aminoacylation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006430 |
lysyl-tRNA aminoacylation |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006430 |
lysyl-tRNA aminoacylation |
IGI: Inferred from Genetic Interaction |
P |
Seeded from EcoCyc (v14.0) |
Missing: with/from | |||
| GO:0016020 |
membrane |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc (v14.0) |
complete | |||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of lysyl-tRNA synthetase (LysRSu), inducible |
could be indirect |
||
|
Protein |
aceE |
Experiment(s):EBI-890346 | ||
|
Protein |
adhE |
Experiment(s):EBI-890346 | ||
|
Protein |
clpB |
Experiment(s):EBI-890346 | ||
|
Protein |
dnaN |
Experiment(s):EBI-890346 | ||
|
Protein |
dps |
Experiment(s):EBI-890346, EBI-895275 | ||
|
Protein |
fusA |
Experiment(s):EBI-890346 | ||
|
Protein |
gadA |
Experiment(s):EBI-890346 | ||
|
Protein |
gadB |
Experiment(s):EBI-890346 | ||
|
Protein |
gapA |
Experiment(s):EBI-890346 | ||
|
Protein |
katE |
Experiment(s):EBI-890346 | ||
|
Protein |
lysA |
Experiment(s):EBI-890346 | ||
|
Protein |
groL |
Experiment(s):EBI-890346 | ||
|
Protein |
pflB |
Experiment(s):EBI-890346, EBI-895275 | ||
|
Protein |
pgk |
Experiment(s):EBI-890346 | ||
|
Protein |
rplB |
Experiment(s):EBI-890346 | ||
|
Protein |
rpoB |
Experiment(s):EBI-890346 | ||
|
Protein |
rpoC |
Experiment(s):EBI-890346 | ||
|
Protein |
rpsB |
Experiment(s):EBI-890346, EBI-895275 | ||
|
Protein |
rpsK |
Experiment(s):EBI-890346 | ||
|
Protein |
tnaA |
Experiment(s):EBI-890346 | ||
|
Protein |
tufA |
Experiment(s):EBI-890346, EBI-895275 | ||
|
Protein |
eno |
Experiment(s):EBI-890346, EBI-895275 | ||
|
Protein |
rplB |
Experiment(s):EBI-1147510 | ||
|
Protein |
ileS |
Experiment(s):EBI-1147510 | ||
|
Protein |
yehI |
Experiment(s):EBI-1147510 | ||
|
Protein |
ydhJ |
Experiment(s):EBI-895275 | ||
|
Protein |
yfcZ |
Experiment(s):EBI-895275 | ||
|
Protein |
cspC |
Experiment(s):EBI-895275 | ||
|
Protein |
cysK |
Experiment(s):EBI-895275 | ||
|
Protein |
focB |
Experiment(s):EBI-895275 | ||
|
Protein |
rplI |
Experiment(s):EBI-895275 | ||
|
Protein |
rplX |
Experiment(s):EBI-895275 | ||
|
Protein |
rplY |
Experiment(s):EBI-895275 | ||
|
Protein |
rpsC |
Experiment(s):EBI-895275 | ||
|
Protein |
rpsG |
Experiment(s):EBI-895275 | ||
|
Protein |
rpsJ |
Experiment(s):EBI-895275 | ||
|
Protein |
serS |
Experiment(s):EBI-895275 | ||
|
Protein |
wcaI |
Experiment(s):EBI-895275 | ||
|
Protein |
wrbA |
Experiment(s):EBI-895275 | ||
|
Protein |
yagJ |
Experiment(s):EBI-895275 | ||
|
Protein |
ygiW |
Experiment(s):EBI-895275 | ||
|
Protein |
nlpI |
Experiment(s):EBI-895275 | ||
|
Protein |
yhjX |
Experiment(s):EBI-895275 | ||
|
Protein |
dnaN |
MALDI(Z-score):25.942724 | ||
|
Protein |
eno |
LCMS(ID Probability):99.0 MALDI(Z-score):17.053160 | ||
|
Protein |
rfaD |
MALDI(Z-score):17.908007 | ||
|
Protein |
gadB |
MALDI(Z-score):17.900703 | ||
|
Protein |
dps |
LCMS(ID Probability):99.0 MALDI(Z-score):39.268839 | ||
|
Protein |
serS |
LCMS(ID Probability):99.0 | ||
|
Protein |
clpB |
MALDI(Z-score):17.053160 | ||
|
Protein |
gapA |
MALDI(Z-score):20.539819 | ||
|
Protein |
yfiD |
LCMS(ID Probability):99.0 MALDI(Z-score):25.080743 | ||
|
Protein |
pflB |
LCMS(ID Probability):99.0 MALDI(Z-score):17.053160 | ||
|
Protein |
rplY |
LCMS(ID Probability):99.0 | ||
|
Protein |
pgk |
MALDI(Z-score):17.053160 | ||
|
Protein |
adhE |
MALDI(Z-score):23.622405 | ||
|
Protein |
tnaA |
MALDI(Z-score):24.637611 | ||
|
Protein |
yfcZ |
LCMS(ID Probability):99.0 | ||
|
Protein |
cysK |
LCMS(ID Probability):99.0 | ||
|
Protein |
wrbA |
LCMS(ID Probability):99.0 MALDI(Z-score):7.204307 | ||
|
Protein |
nlpI |
LCMS(ID Probability):99.0 | ||
|
Protein |
yhjX |
LCMS(ID Probability):99.0 | ||
|
Protein |
focB |
LCMS(ID Probability):99.0 | ||
|
Protein |
ydhJ |
LCMS(ID Probability):99.0 | ||
| edit table |
</protect>
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
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Notes
Structure and Physical Properties
<protect>
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MSEQETRGAN EAIDFNDELR NRREKLAALR QQGVAFPNDF RRDHTSDQLH EEFDAKDNQE LESLNIEVSV AGRMMTRRIM GKASFVTLQD VGGRIQLYVA RDSLPEGVYN DQFKKWDLGD IIGARGTLFK TQTGELSIHC TELRLLTKAL RPLPDKFHGL QDQEVRYRQR YLDLIANDKS RQTFVVRSKI LAAIRQFMVA RGFMEVETPM MQVIPGGASA RPFITHHNAL DLDMYLRIAP ELYLKRLVVG GFERVFEINR NFRNEGISVR HNPEFTMMEL YMAYADYHDL IELTESLFRT LAQEVLGTTK VTYGEHVFDF GKPFEKLTMR EAIKKYRPET DMADLDNFDA AKALAESIGI TVEKSWGLGR IVTEIFDEVA EAHLIQPTFI TEYPAEVSPL ARRNDVNPEI TDRFEFFIGG REIGNGFSEL NDAEDQAERF QEQVNAKAAG DDEAMFYDED YVTALEYGLP PTAGLGIGID RMIMLFTNSH TIRDVILFPA MRPQK |
| Length |
505 |
| Mol. Wt |
57.828 kDa |
| pI |
5.0 (calculated) |
| Extinction coefficient |
33,350 - 33,475 (calc based on 15 Y, 2 W, and 1 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 Brevet, A et al. (1995) Comparison of the enzymatic properties of the two Escherichia coli lysyl-tRNA synthetase species. J. Biol. Chem. 270 14439-44 PubMed
- ↑ 6.0 6.1 Desogus, G et al. (2000) Active site of lysyl-tRNA synthetase: structural studies of the adenylation reaction. Biochemistry 39 8418-25 PubMed
- ↑ 7.0 7.1 Valent, QA et al. (1995) Early events in preprotein recognition in E. coli: interaction of SRP and trigger factor with nascent polypeptides. EMBO J. 14 5494-505 PubMed
- ↑ Charlier, J & Sanchez, R (1987) Lysyl-tRNA synthetase from Escherichia coli K12. Chromatographic heterogeneity and the lysU-gene product. Biochem. J. 248 43-51 PubMed
- ↑ 9.0 9.1 Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
- ↑ 10.00 10.01 10.02 10.03 10.04 10.05 10.06 10.07 10.08 10.09 10.10 10.11 10.12 10.13 10.14 10.15 10.16 10.17 10.18 10.19 10.20 10.21 10.22 10.23 10.24 10.25 10.26 10.27 10.28 10.29 10.30 10.31 10.32 10.33 10.34 10.35 10.36 10.37 10.38 10.39 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ 11.0 11.1 11.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 12.00 12.01 12.02 12.03 12.04 12.05 12.06 12.07 12.08 12.09 12.10 12.11 12.12 12.13 12.14 12.15 12.16 12.17 12.18 12.19 12.20 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ Macek, B et al. (2008) Phosphoproteome analysis of E. coli reveals evolutionary conservation of bacterial Ser/Thr/Tyr phosphorylation. Mol. Cell Proteomics 7 299-307 PubMed
- ↑ 14.0 14.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0000166 ! nucleotide binding
- GO:0000287 ! magnesium ion binding
- GO:0016874 ! ligase activity
- GO:0003676 ! nucleic acid binding
- GO:0004824 ! lysine-tRNA ligase activity
- GO:0006418 ! tRNA aminoacylation for protein translation
- GO:0006430 ! lysyl-tRNA aminoacylation
- GO:0005524 ! ATP binding
- GO:0006412 ! translation
- GO:0005737 ! cytoplasm
- GO:0005829 ! cytosol
- GO:0016020 ! membrane
- Proteins
- RefGenome Annotated Gene
