lpxD:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

lpxD

Gene Synonym(s)

ECK0178, b0179, JW0174, fir, firA, hlpA[1], ssc, omsA, osmA[2][3]

Product Desc.

UDP-3-O-(3-hydroxymyristoyl)-glucosamine N-acyltransferase

Lipid A biosynthesis, UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase[4]

Product Synonyms(s)

UDP-3-O-(3-hydroxymyristoyl)-glucosamine N-acyltransferase[2], B0179[3][2], OmsA[3][2], Ssc[3][2], OsmA[3][2], FirA[3][2], LpxD[3][2] , ECK0178, firA, JW0174, omsA, ssc, b0179

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s):

Regulation/Activity

LPS biosynthesis

Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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LpxD contains 25 hexapeptide repeats. The lpxD gene is essential.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

lpxD

Mnemonic

Lipid A expression/biosynthesis

Synonyms

ECK0178, b0179, JW0174, fir, firA, hlpA[1], ssc, omsA, osmA[2][3]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

4.33 minutes 

MG1655: 200971..201996
<gbrowseImage> name=NC_000913:200971..201996 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 203813..204838
<gbrowseImage> name=NC_012967:203813..204838 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 200970..201995
<gbrowseImage> name=NC_012759:200970..201995 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 200971..201996
<gbrowseImage> name=NC_007779:200971..201996 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 175075..176100
<gbrowseImage> name=NC_010473:175075..176100 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

200974

Edman degradation

PMID:2115869


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

lpxDQ165R

Q165R

In firA200; confer temperature sensitivity and reverses the rifampicin resistance of rpoB mutants

seeded from UniProt:P21645

lpxDI172F

I172F

In firA201; confer temperature sensitivity

seeded from UniProt:P21645

lpxDG228D

G228D

In firA200 and firA201; confer temperature sensitivity

seeded from UniProt:P21645

lpxDG252S

G252S

In firA200 and firA201; confer temperature sensitivity

seeded from UniProt:P21645

lpxDS271N

S271N

In omsA; confer temperature sensitivity

seeded from UniProt:P21645

lpxD36(ts)

temperature sensitive

PMID:6447875

CGSC:38940

lpxD200(ts)

temperature sensitive

CGSC:7545


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Notes

Genetic Interactions

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<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0174

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCCTTCAATTCGACTGGCTGA

Primer 2:CCGTCTTGTTGATTAACCTTGCG

E2B8

Kohara Phage

Genobase

PMID:3038334

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[5]

est. P1 cotransduction: 8% [6]

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[5]

est. P1 cotransduction: 30% [6]
Synonyms:zae-502::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10316

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10316

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000310

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944882

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0312

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000610

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

LpxD

Synonyms

UDP-3-O-(3-hydroxymyristoyl)-glucosamine N-acyltransferase[2], B0179[3][2], OmsA[3][2], Ssc[3][2], OsmA[3][2], FirA[3][2], LpxD[3][2] , ECK0178, firA, JW0174, omsA, ssc, b0179

Product description

UDP-3-O-(3-hydroxymyristoyl)-glucosamine N-acyltransferase

Lipid A biosynthesis, UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase[4]

EC number (for enzymes) 
2.3.1.-[2]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [2].

Missing: evidence, reference

GO:0009245

lipid A biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00523

P

Seeded from EcoCyc (v14.0)

complete

GO:0009245

lipid A biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007691

P

Seeded from EcoCyc (v14.0)

complete

GO:0009245

lipid A biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020573

P

Seeded from EcoCyc (v14.0)

complete

GO:0009245

lipid A biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0441

P

Seeded from EcoCyc (v14.0)

complete

GO:0046677

response to antibiotic

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0046

P

Seeded from EcoCyc (v14.0)

complete

GO:0016410

N-acyltransferase activity

PMID:3288280

IDA: Inferred from Direct Assay

F

complete

GO:0009245

lipid A biosynthetic process

PMID:8366125

IMP: Inferred from Mutant Phenotype

P

complete

GO:0016410

N-acyltransferase activity

PMID:8366125

IDA: Inferred from Direct Assay

F

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

uniparc:UPI00000D382C

PMID:15690043

Experiment(s):EBI-892599

Protein

ydfR

PMID:15690043

Experiment(s):EBI-886059

Protein

cbpA

PMID:15690043

Experiment(s):EBI-886059, EBI-892599

Protein

glnS

PMID:15690043

Experiment(s):EBI-886059

Protein

pssA

PMID:15690043

Experiment(s):EBI-886059

Protein

hldD

PMID:15690043

Experiment(s):EBI-886059

Protein

rho

PMID:15690043

Experiment(s):EBI-886059

Protein

rplB

PMID:15690043

Experiment(s):EBI-886059

Protein

rplD

PMID:15690043

Experiment(s):EBI-886059, EBI-892599

Protein

rplI

PMID:15690043

Experiment(s):EBI-886059, EBI-892599

Protein

rplO

PMID:15690043

Experiment(s):EBI-886059, EBI-892599

Protein

rplU

PMID:15690043

Experiment(s):EBI-886059, EBI-892599

Protein

rpsB

PMID:15690043

Experiment(s):EBI-886059, EBI-892599

Protein

rpsC

PMID:15690043

Experiment(s):EBI-886059, EBI-892599

Protein

rpsD

PMID:15690043

Experiment(s):EBI-886059, EBI-892599

Protein

rpsE

PMID:15690043

Experiment(s):EBI-886059, EBI-892599

Protein

rpsG

PMID:15690043

Experiment(s):EBI-886059, EBI-892599

Protein

tyrA

PMID:15690043

Experiment(s):EBI-886059

Protein

rluB

PMID:15690043

Experiment(s):EBI-886059

Protein

lpxA

PMID:16606699

Experiment(s):EBI-1135798

Protein

rpsD

PMID:16606699

Experiment(s):EBI-1135798

Protein

rpsG

PMID:16606699

Experiment(s):EBI-1135798

Protein

rplR

PMID:16606699

Experiment(s):EBI-1135798

Protein

rplB

PMID:16606699

Experiment(s):EBI-1135798

Protein

rpsR

PMID:16606699

Experiment(s):EBI-1135798

Protein

rpsI

PMID:16606699

Experiment(s):EBI-1135798

Protein

sdhA

PMID:16606699

Experiment(s):EBI-1135798

Protein

ycbY

PMID:16606699

Experiment(s):EBI-1135798

Protein

rpsM

PMID:16606699

Experiment(s):EBI-1135798

Protein

rplA

PMID:16606699

Experiment(s):EBI-1135798

Protein

rpsC

PMID:16606699

Experiment(s):EBI-1135798

Protein

phnA

PMID:16606699

Experiment(s):EBI-1135798

Protein

rplD

PMID:16606699

Experiment(s):EBI-1135798

Protein

rpoC

PMID:16606699

Experiment(s):EBI-1135798

Protein

aspA

PMID:15690043

Experiment(s):EBI-892599

Protein

csdA

PMID:15690043

Experiment(s):EBI-892599

Protein

ccmB

PMID:15690043

Experiment(s):EBI-892599

Protein

dnaJ

PMID:15690043

Experiment(s):EBI-892599

Protein

rnk

PMID:15690043

Experiment(s):EBI-892599

Protein

rplC

PMID:15690043

Experiment(s):EBI-892599

Protein

rplK

PMID:15690043

Experiment(s):EBI-892599

Protein

rplL

PMID:15690043

Experiment(s):EBI-892599

Protein

rplM

PMID:15690043

Experiment(s):EBI-892599

Protein

rplP

PMID:15690043

Experiment(s):EBI-892599

Protein

rplS

PMID:15690043

Experiment(s):EBI-892599

Protein

rplT

PMID:15690043

Experiment(s):EBI-892599

Protein

rplV

PMID:15690043

Experiment(s):EBI-892599

Protein

rplX

PMID:15690043

Experiment(s):EBI-892599

Protein

rpmG

PMID:15690043

Experiment(s):EBI-892599

Protein

rpsH

PMID:15690043

Experiment(s):EBI-892599

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-892599

Protein

rpsP

PMID:15690043

Experiment(s):EBI-892599

Protein

rpsR

PMID:15690043

Experiment(s):EBI-892599

Protein

rpsT

PMID:15690043

Experiment(s):EBI-892599

Protein

yfiF

PMID:15690043

Experiment(s):EBI-892599

Protein

yhbY

PMID:15690043

Experiment(s):EBI-892599

Protein

yjfI

PMID:15690043

Experiment(s):EBI-892599

Protein

ydgI

PMID:15690043

Experiment(s):EBI-892599

Protein

rplP

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):5.589311

Protein

rfaD

PMID:19402753

MALDI(Z-score):39.513239

Protein

ccmB

PMID:19402753

LCMS(ID Probability):99.0

Protein

rplO

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

Protein

rplU

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

Protein

rplI

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

Protein

rho

PMID:19402753

MALDI(Z-score):21.565908

Protein

rplA

PMID:19402753

MALDI(Z-score):24.027450

Protein

rplB

PMID:19402753

MALDI(Z-score):34.741324

Protein

aspA

PMID:19402753

LCMS(ID Probability):99.0

Protein

rpsC

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):25.212997

Protein

rpsD

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):36.130305

Protein

pssA

PMID:19402753

MALDI(Z-score):27.424997

Protein

cbpA

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):19.850514

Protein

rplD

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):20.126458

Protein

csdA

PMID:19402753

LCMS(ID Probability):99.0

Protein

yfiF

PMID:19402753

LCMS(ID Probability):99.0

Protein

rluB

PMID:19402753

MALDI(Z-score):31.976205

Protein

yjfI

PMID:19402753

LCMS(ID Probability):99.0

Protein

rnk

PMID:19402753

LCMS(ID Probability):99.0

Protein

ydgI

PMID:19402753

LCMS(ID Probability):99.0

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MPSIRLADLA QQLDAELHGD GDIVITGVAS MQSAQTGHIT FMVNPKYREH LGLCQASAVV
MTQDDLPFAK SAALVVKNPY LTYARMAQIL DTTPQPAQNI APSAVIDATA KLGNNVSIGA
NAVIESGVEL GDNVIIGAGC FVGKNSKIGA GSRLWANVTI YHEIQIGQNC LIQSGTVVGA
DGFGYANDRG NWVKIPQIGR VIIGDRVEIG ACTTIDRGAL DDTIIGNGVI IDNQCQIAHN
VVIGDNTAVA GGVIMAGSLK IGRYCMIGGA SVINGHMEIC DKVTVTGMGM VMRPITEPGV
YSSGIPLQPN KVWRKTAALV MNIDDMSKRL KSLERKVNQQ D
Length

341

Mol. Wt

36.037 kDa

pI

6.5 (calculated)

Extinction coefficient

26,930 - 27,805 (calc based on 7 Y, 3 W, and 7 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P21645

Domain

127..144

PF00132 Bacterial transferase hexapeptide (three repeats)

PMID:19920124

Domain

21..90

PF04613 UDP-3-O-[3-hydroxymyristoyl glucosamine N-acyltransferase, LpxD ]

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lpxD taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128172

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944882

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000610

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P21645

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10316

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10316

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944882

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000310

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0312

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

4.53E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

96.296+/-0.39

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.14095+/-0.01151

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by FISH

PMID:20671182

mRNA

Ecoli K-12

0.408780488

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

4225

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

982

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

2637

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

yaeT-hlpA-lpxD-fabZ-lpxAB-rnhB-dnaE

hlpA-lpxD-fabZ-lpxA

lpxD

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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:200951..200991 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0179 (EcoliWiki Page)

NCBI GEO profiles for lpxD

microarray

GenExpDB:b0179 (EcoliWiki Page)

Summary of data for lpxD from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to lpxD Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10316

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0312

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0179

EcoGene

EcoGene:EG10316

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000310

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000610

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Arabidopsis thaliana

  • AT4G21220 (score: 1.000; bootstrap: 100%)
  • AT4G05210 (score: 0.483)

From Inparanoid:20070104

Shigella flexneri

LPXD

From SHIGELLACYC

E. coli O157

LPXD

From ECOO157CYC

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00132 Bacterial transferase hexapeptide (three repeats)

Pfam (EcoliWiki Page)

PF04613 UDP-3-O-[3-hydroxymyristoyl glucosamine N-acyltransferase, LpxD ]

Superfamily (EcoliWiki Page)

SUPERFAMILY:51161

Panther (EcoliWiki Page)

PTHR22572:SF46

EcoCyc

EcoCyc:EG10316

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10316

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000310

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0312

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000610

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 Dicker, IB & Seetharam, S (1991) Cloning and nucleotide sequence of the firA gene and the firA200(Ts) allele from Escherichia coli. J. Bacteriol. 173 334-44 PubMed
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  3. ↑ 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 3.14 3.15 3.16 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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