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Expression|

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Standard Name	lolA
Gene Synonym(s)	ECK0882, b0891, JW0874, yzzV^[1], lplA, yzzV
Product Desc.	periplasmic chaperone, effects translocation of lipoproteins from inner membrane to outer^[2]^[3] Periplasmic protein responsible for sorting and transporting lipoproteins to outer membrane^[4]
Product Synonyms(s)	chaperone for lipoproteins^[1], B0891^[2]^[1], YzzV^[2]^[1], LolA^[2]^[1], p20^[2]^[1] , ECK0882, JW0874, lplA, yzzV, b0891
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): lolA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	lolA
Mnemonic	Localization OM lipoproteins
Synonyms	ECK0882, b0891, JW0874, yzzV^[1], lplA, yzzV
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	20.19 minutes	MG1655: 936592..937206 <gbrowseImage> name=NC_000913:936592..937206 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 954462..955076 <gbrowseImage> name=NC_012967:954462..955076 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 839563..840174 <gbrowseImage> name=NC_012759:839563..840174 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 937791..938405 <gbrowseImage> name=NC_007779:937791..938405 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 990523..991134 <gbrowseImage> name=NC_010473:990523..991134 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	936661	Edman degradation	PMID:7628437 PMID:9298646
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	AA change(s)	Phenotype: Description	Comments
lolAR64L	R64L	Loss of ability to transfer lipoproteins to lolB	seeded from UniProt:P61316
lolAF68E	F68E	Loss of ability to bind lipoproteins	seeded from UniProt:P61316
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0874	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCATGAAAAAAATTGCCATCAC Primer 2:CCCTTACGTTGATCATCTACCGT
1F10	Kohara Phage	Genobase	PMID:3038334
E6H3	Kohara Phage	Genobase	PMID:3038334
zbh-29::Tn10	Linked marker	CAG18493 = CGSC7353^[5]	est. P1 cotransduction: % ^[6] Synonyms:zbi-29::Tn10
zca-1230::Tn10	Linked marker	CAG18478 = CGSC7356^[5]	est. P1 cotransduction: 71% ^[6] Synonyms:zbj-1230::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6465	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12684	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003065	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948989	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2548	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003031	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	LolA
Synonyms	chaperone for lipoproteins^[1], B0891^[2]^[1], YzzV^[2]^[1], LolA^[2]^[1], p20^[2]^[1] , ECK0882, JW0874, lplA, yzzV, b0891
Product description	periplasmic chaperone, effects translocation of lipoproteins from inner membrane to outer^[2]^[3] Periplasmic protein responsible for sorting and transporting lipoproteins to outer membrane^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008565	protein transporter activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00240	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015031	protein transport	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00240	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015031	protein transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004564	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015031	protein transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018323	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015031	protein transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0653	P	Seeded from EcoCyc (v14.0)	complete
	GO:0030288	outer membrane-bounded periplasmic space	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004564	C	Seeded from EcoCyc (v14.0)	complete
	GO:0030288	outer membrane-bounded periplasmic space	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018323	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00240	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042953	lipoprotein transport	PMID:7628437	IDA: Inferred from Direct Assay		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	aceE	PMID:15690043	Experiment(s):EBI-881219
Protein	lon	PMID:15690043	Experiment(s):EBI-881219
Protein	rpsJ	PMID:15690043	Experiment(s):EBI-881219
Protein	ygdI	PMID:15690043	Experiment(s):EBI-888472
Protein	rplL	PMID:15690043	Experiment(s):EBI-888472
Protein	rplM	PMID:15690043	Experiment(s):EBI-888472
Protein	rplU	PMID:15690043	Experiment(s):EBI-888472
Protein	rplV	PMID:15690043	Experiment(s):EBI-888472
Protein	rplM	PMID:19402753	LCMS(ID Probability):99.6
Protein	ygdI	PMID:19402753	LCMS(ID Probability):99.6
Protein	rplL	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
periplasm
Periplasm	PMID:7628437	EchoLocation:lolA
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMKKIAITCA LLSSLVASSV WADAASDLKS RLDKVSSFHA SFTQKVTDGS GAAVQEGQGD LWVKRPNLFN WHMTQPDESI LVSDGKTLWF YNPFVEQATA TWLKDATGNT PFMLIARNQS SDWQQYNIKQ NGDDFVLTPK ASNGNLKQFT INVGRDGTIH QFSAVEQDDQ RSSYQLKSQQ NGAVDAAKFT FTPPQGVTVD DQRK
Length	204
Mol. Wt	22.627 kDa
pI	6.7 (calculated)
Extinction coefficient	37,470 - 37,595 (calc based on 3 Y, 6 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-21	UniProt Manual:Signal Peptides	UniProt:P61316
Domain	32..191	PF03548 Outer membrane lipoprotein carrier protein LolA		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=lolA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:145698236	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948989	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003031	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P61316	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6465	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12684	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948989	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003065	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2548	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	6.13E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	2.87+/-0.047	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.109934853	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	1198	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	741	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	992	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
lolA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:936572..936612 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0891 (EcoliWiki Page)	NCBI GEO profiles for lolA
microarray	GenExpDB:b0891 (EcoliWiki Page)	Summary of data for lolA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (936354..936674) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ13; Well:F11^[7]
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Notes

Accessions Related to lolA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6465	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2548	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0891
EcoGene	EcoGene:EG12684	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003065	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003031	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000010304 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000014806 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	LOLA	From SHIGELLACYC
E. coli O157	LOLA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF03548 Outer membrane lipoprotein carrier protein LolA
Superfamily (EcoliWiki Page)	SUPERFAMILY:89392
EcoCyc	EcoCyc:G6465	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12684	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003065	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2548	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003031	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

lolA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to lolA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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