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On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	leuA
Gene Synonym(s)	ECK0076, b0074, JW0073^[1], JW0073
Product Desc.	2-isopropylmalate synthase^[2]^[3] alpha-Isopropylmalate synthase^[4]
Product Synonyms(s)	2-isopropylmalate synthase^[1], B0074^[2]^[1], LeuA^[2]^[1] , ECK0076, JW0073, b0074
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): leuLABCD^[2], OP00249, leu, leuABCD
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	leuA
Mnemonic	Leucine (biosynthesis)
Synonyms	ECK0076, b0074, JW0073^[1], JW0073
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	1.77 minutes	MG1655: 83529..81958 <gbrowseImage> name=NC_000913:81958..83529 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 86333..84762 <gbrowseImage> name=NC_012967:84762..86333 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 81957..83528 <gbrowseImage> name=NC_012759:81957..83528 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 83529..81958 <gbrowseImage> name=NC_007779:81958..83529 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	81961	Edman degradation	PMID:9298646
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability
leuA(del) (Keio:JW0073)	deletion	deletion			PMID:16738554	Shigen CGSC8373^[5]
leuA371			Auxotrophies	requires leucine for growth		CGSC:6576
leuA781(del)::kan					PMID:16738554	CGSC:101648
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0073	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGCCAGCAAGTCATTATTTT Primer 2:CCCACGGTTTCCTTGTTGTTTTC
8H11	Kohara Phage	Genobase	PMID:3038334
6C1	Kohara Phage	Genobase	PMID:3038334
carB96::Tn10	Linked marker	CAG12093 = CGSC7322^[5]	est. P1 cotransduction: 10% ^[6] Synonyms:car-96::Tn10
leuO3051::Tn10	Linked marker	CAG12095 = CGSC7324^[5]	est. P1 cotransduction: 95% ^[6] Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11226	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11226	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001204	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947465	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1208	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000267	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	LeuA
Synonyms	2-isopropylmalate synthase^[1], B0074^[2]^[1], LeuA^[2]^[1] , ECK0076, JW0073, b0074
Product description	2-isopropylmalate synthase^[2]^[3] alpha-Isopropylmalate synthase^[4]
EC number (for enzymes)	4.1.3.12^[1]	Has been transferred to EC-Number 2.3.3.13
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003852	2-isopropylmalate synthase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01025	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003852	2-isopropylmalate synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005671	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003852	2-isopropylmalate synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013709	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003852	2-isopropylmalate synthase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.3.3.13	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009098	leucine biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01025	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009098	leucine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005671	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009098	leucine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013709	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009098	leucine biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0432	P	Seeded from EcoCyc (v14.0)	complete
	GO:0046912	transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002034	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009098	leucine biosynthetic process	PMID:4570778	IMP: Inferred from Mutant Phenotype		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	fdnH	PMID:16606699	Experiment(s):EBI-1135508
Protein	dnaK	PMID:16606699	Experiment(s):EBI-1135508
Protein	htpG	PMID:16606699	Experiment(s):EBI-1135508
Protein	nadE	PMID:16606699	Experiment(s):EBI-1135508
Protein	phoQ	PMID:16606699	Experiment(s):EBI-1135508
Protein	sucD	PMID:19402753	LCMS(ID Probability):99.6
Protein	sucC	PMID:19402753	LCMS(ID Probability):99.6
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Notes

Localization

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Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSQQVIIFDT TLRDGEQALQ ASLSVKEKLQ IALALERMGV DVMEVGFPVS SPGDFESVQT IARQVKNSRV CALARCVEKD IDVAAESLKV AEAFRIHTFI ATSPMHIATK LRSTLDEVIE RAIYMVKRAR NYTDDVEFSC EDAGRTPIAD LARVVEAAIN AGATTINIPD TVGYTMPFEF AGIISGLYER VPNIDKAIIS VHTHDDLGLA VGNSLAAVHA GARQVEGAMN GIGERAGNCS LEEVIMAIKV RKDILNVHTA INHQEIWRTS QLVSQICNMP IPANKAIVGS GAFAHSSGIH QDGVLKNREN YEIMTPESIG LNQIQLNLTS RSGRAAVKHR MDEMGYKESE YNLDNLYDAF LKLADKKGQV FDYDLEALAF IGKQQEEPEH FRLDYFSVQS GSNDIATAAV KLACGEEVKA EAANGNGPVD AVYQAINRIT EYNVELVKYS LTAKGHGKDA LGQVDIVANY NGRRFHGVGL ATDIVESSAK AMVHVLNNIW RAAEVEKELQ RKAQHNENNK ETV
Length	523
Mol. Wt	57.297 kDa
pI	5.6 (calculated)
Extinction coefficient	31,860 - 32,610 (calc based on 14 Y, 2 W, and 6 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P09151
Domain	13..252	PF00682 HMGL-like		PMID:19920124
Domain	369..500	PF08502 LeuA allosteric (dimerisation) domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=leuA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128068	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947465	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000267	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P09151	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11226	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11226	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947465	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001204	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1208	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	6.64E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	E. coli K-12 EMG2	160	molecules/cell	Medium: MOPS glucose minimal Temperature (°C): 37 Doubling time (min): Growth phase: Exponential OD: OD600 = 0.2 to 0.25 other: aerated	Quantitative protein sequencing	Spot ID: M129	PMID: 9298646
Protein	E. coli K-12 MG1655	337	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	2685	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	579	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
leuLABCD
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:83509..83549 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0074 (EcoliWiki Page)	NCBI GEO profiles for leuA
microarray	GenExpDB:b0074 (EcoliWiki Page)	Summary of data for leuA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to leuA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11226	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1208	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0074
EcoGene	EcoGene:EG11226	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001204	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000267	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000011126 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT1G74040 (score: 1.000; bootstrap: 100%) AT1G18500 (score: 0.798) AT5G23010 (score: 0.194) AT5G23020 (score: 0.188)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000029103 (score: 1.000; bootstrap: 100%) ENSBTAP00000019954 (score: 0.085)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000019305 (score: 1.000; bootstrap: 100%) ENSCAFP00000003388 (score: 0.630)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000011608 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-040426-958 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040425-1889 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0230167 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0031877 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA10298-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000006445 (score: 1.000; bootstrap: 100%) ENSGALP00000026228 (score: 0.693)	From Inparanoid:20070104
Homo sapiens	ENSP00000235958 (score: 1.000; bootstrap: 100%) ENSP00000274901 (score: 0.668)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000025677 (score: 1.000; bootstrap: 100%) ENSMMUP00000001867 (score: 0.651)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000023425 (score: 1.000; bootstrap: 100%) ENSMODP00000016125 (score: 0.605)	From Inparanoid:20070104
Mus musculus	MGI:96158 (score: 1.000; bootstrap: 100%) MGI:2446108 (score: 0.648)	From Inparanoid:20070104
Oryza gramene	Q5NBI8 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000000589 (score: 1.000; bootstrap: 100%) ENSPTRP00000041348 (score: 0.430)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000012853 (score: 1.000; bootstrap: 100%) ENSRNOP00000014927 (score: 0.660)	From Inparanoid:20070104
Saccharomyces cerevisiae	YDL131W (score: 1.000; bootstrap: 56%) YDL182W (score: 1.000; bootstrap: 55%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC11052c (score: 1.000; bootstrap: 75%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000136985 (score: 1.000; bootstrap: 59%) NEWSINFRUP00000134746 (score: 0.675)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00031359001 (score: 1.000; bootstrap: 100%) GSTENP00030684001 (score: 0.518)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000007177 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	LEUA	From SHIGELLACYC
E. coli O157	LEUA	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00682 HMGL-like
Superfamily (EcoliWiki Page)	SUPERFAMILY:51569
Panther (EcoliWiki Page)	PTHR10277:SF9
Superfamily (EcoliWiki Page)	SUPERFAMILY:110921
Pfam (EcoliWiki Page)	PF08502 LeuA allosteric (dimerisation) domain
EcoCyc	EcoCyc:EG11226	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11226	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001204	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1208	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0000267	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

leuA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to leuA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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