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Expression|

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Standard Name	kptA
Gene Synonym(s)	ECK4322, b4331, JW5784, yjiI^[1], yjiI
Product Desc.	enzyme with tRNA 2'-phosphotransferase activity^[2]^[3] 2'-phosphotransferase, ligated tRNA substrate in vitro, physiological role and in vivo substrate unknown^[4]
Product Synonyms(s)	2'-phosphotransferase^[1], B4331^[2]^[1], YjiI^[2]^[1], KptA^[2]^[1] , ECK4322, JW5784, yjiI, b4331
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): kptA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	kptA
Mnemonic	K-12 phosphotransferase
Synonyms	ECK4322, b4331, JW5784, yjiI^[1], yjiI
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	98.26 minutes, 98.26 minutes	MG1655: 4558953..4559507 <gbrowseImage> name=NC_000913:4558953..4559507 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4541844..4542398 <gbrowseImage> name=NC_012967:4541844..4542398 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4497438..4497992 <gbrowseImage> name=NC_012759:4497438..4497992 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4565610..4566164 <gbrowseImage> name=NC_007779:4565610..4566164 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele

Nt change(s)

AA change(s)

Phenotype: Type

Phenotype: Description

Reference

Availability

Comments

kptA(del) (Keio:JW5784)

deletion

PMID:16738554

Shigen

CGSC11576^[5]

kptA729(del)::kan

PMID:16738554

CGSC:101632

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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5784	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCAAAGTACAACGAAAAAGA Primer 2:CCCCACTCAAGAAACTCAACCGC
5G8	Kohara Phage	Genobase	PMID:3038334
8H8	Kohara Phage	Genobase	PMID:3038334
zjf-920::Tn10	Linked marker	CAG12019 = CGSC7484^[5]	est. P1 cotransduction: % ^[6] Synonyms:zjh-901::Tn10, zjg-920::Tn10
mdoB202::Tn10	Linked marker	CAG18430 = CGSC7488^[5]	est. P1 cotransduction: 25% ^[6] Synonyms:zjj-202::Tn10, zji-202::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7928	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12570	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004423	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948858	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2458	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014204	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	KptA
Synonyms	2'-phosphotransferase^[1], B4331^[2]^[1], YjiI^[2]^[1], KptA^[2]^[1] , ECK4322, JW5784, yjiI, b4331
Product description	enzyme with tRNA 2'-phosphotransferase activity^[2]^[3] 2'-phosphotransferase, ligated tRNA substrate in vitro, physiological role and in vivo substrate unknown^[4]
EC number (for enzymes)	2.7.-.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006388	tRNA splicing, via endonucleolytic cleavage and ligation	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00299	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006388	tRNA splicing, via endonucleolytic cleavage and ligation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002745	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016740	transferase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0808	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016772	transferase activity, transferring phosphorus-containing groups	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00299	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016772	transferase activity, transferring phosphorus-containing groups	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002745	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MWKRLCAGLS CRKFINQVTM TSVNKNRKSR DENEMAKYNE KELADTSKFL SFVLRHKPEA IGIVLDREGW ADIDKLILCA QKAGKRLTRA LLDTVVATSD KKRFSYSSDG RCIRAVQGHS TSQVAISFAE KTPPQFLYHG TASRFLDEIK KQGLIAGERH YVHLSADEAT ARKVGARHGS PVILTVKAQE MAKRGLPFWQ AENGVWLTST VAVEFLEW
Length	218
Mol. Wt	24.556 kDa
pI	10.0 (calculated)
Extinction coefficient	33,460 - 33,960 (calc based on 4 Y, 5 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	5..180	PF01885 RNA 2'-phosphotransferase, Tpt1 / KptA family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=kptA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:90111732	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948858	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014204	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P39380	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7928	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12570	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948858	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004423	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2458	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	8^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	6^a	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	1^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
kptA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4558933..4558973 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4331 (EcoliWiki Page)	NCBI GEO profiles for kptA
microarray	GenExpDB:b4331 (EcoliWiki Page)	Summary of data for kptA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to kptA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7928	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2458	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4331
EcoGene	EcoGene:EG12570	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004423	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014204	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000035359 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT5G23600 (score: 1.000; bootstrap: 100%) AT2G45330 (score: 0.921)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000022022 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000004882 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0187196 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0053057 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000314073 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000016989 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000012807 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000006568 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000031211 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YOL102C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC2C42c (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000133489 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00005105001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000015639 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YJII	From SHIGELLACYC
E. coli O157	Z5930	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Panther (EcoliWiki Page)	PTHR12684:SF2
Superfamily (EcoliWiki Page)	SUPERFAMILY:56399
Pfam (EcoliWiki Page)	PF01885 RNA 2'-phosphotransferase, Tpt1 / KptA family
EcoCyc	EcoCyc:G7928	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12570	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120004423	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2458	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014204	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

kptA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to kptA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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