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Gene |

Product(s) |

Expression|

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Standard Name	intD
Gene Synonym(s)	ECK0530, b0537, JW0525, int^[1], int
Product Desc.	DLP12 prophage; predicted integrase^[2]^[3] Integrase gene within defective prophage DLP12^[4]
Product Synonyms(s)	DLP12 prophage; predicted integrase^[1], B0537^[2]^[1], Int^[2]^[1], IntD^[2]^[1] , ECK0530, int, JW0525, b0537
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): intD^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	intD
Mnemonic	Integrase
Synonyms	ECK0530, b0537, JW0525, int^[1], int
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	12.16 minutes	MG1655: 565201..564038 <gbrowseImage> name=NC_000913:564038..565201 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 538092..536929 <gbrowseImage> name=NC_012967:536929..538092 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 466798..467961 <gbrowseImage> name=NC_012759:466798..467961 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 565201..564038 <gbrowseImage> name=NC_007779:564038..565201 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 504533..503370 <gbrowseImage> name=NC_010473:503370..504533 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔintD (Keio:JW0525)	deletion	deletion	PMID:16738554	Shigen
intD::Tn5KAN-2 (FB20199)	Insertion at nt 524 in Minus orientation		PMID:15262929	E. coli Genome Project:FB20199	does not contain pKD46
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0525	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCACTCTTCCGCAGAAATGA Primer 2:CCCGCCTTCTTTATATCCTCCAT
2C4	Kohara Phage	Genobase	PMID:3038334
purK79::Tn10	Linked marker	CAG12171 = CGSC7342^[5]	est. P1 cotransduction: 66% ^[6] Synonyms:purE79::Tn10
dsbG601::Tn10	Linked marker	CAG12149 = CGSC7346^[5]	est. P1 cotransduction: 1% ^[6] Synonyms:zbd-601::Tn10, zbe-601::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10507	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10507	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000500	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947162	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0502	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001842	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	IntD
Synonyms	DLP12 prophage; predicted integrase^[1], B0537^[2]^[1], Int^[2]^[1], IntD^[2]^[1] , ECK0530, int, JW0525, b0537
Product description	DLP12 prophage; predicted integrase^[2]^[3] Integrase gene within defective prophage DLP12^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002104	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011010	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003677	DNA binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013762	F	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002104	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013762	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006310	DNA recombination	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0233	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015074	DNA integration	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002104	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015074	DNA integration	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013762	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015074	DNA integration	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0229	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	ynaE	PMID:16606699	Experiment(s):EBI-1137105
Protein	paaG	PMID:16606699	Experiment(s):EBI-1137105
Protein	ydfK	PMID:16606699	Experiment(s):EBI-1137105
Protein	rpsI	PMID:16606699	Experiment(s):EBI-1137105
Protein	rpsD	PMID:16606699	Experiment(s):EBI-1137105
Protein	yiiQ	PMID:16606699	Experiment(s):EBI-1137105
Protein	gadX	PMID:16606699	Experiment(s):EBI-1137105
Protein	rplD	PMID:16606699	Experiment(s):EBI-1137105
Protein	rof	PMID:16606699	Experiment(s):EBI-1137105
Protein	ruvB	PMID:16606699	Experiment(s):EBI-1137105
Protein	dinG	PMID:16606699	Experiment(s):EBI-1137105
Protein	crl	PMID:16606699	Experiment(s):EBI-1137105
Protein	ribH	PMID:16606699	Experiment(s):EBI-1137105
Protein	yeaO	PMID:16606699	Experiment(s):EBI-1137105
Protein	rplQ	PMID:16606699	Experiment(s):EBI-1137105
Protein	rplB	PMID:16606699	Experiment(s):EBI-1137105
Protein	phnA	PMID:16606699	Experiment(s):EBI-1137105
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSLFRRNEIW YASYSLPGGK RIKESLGTKD KRQAQELHDK RKAELWRVEK LGDLPDVTFE EACLRWLEEK ADKKSLDSDK SRIEFWLEHF EGIRLKDISE AKIYSAVSRM HNRKTKEIWK QKVQAAIRKG KELPVYEPKP VSTQTKAKHL AMIKAILRAA ERDWKWLEKA PVIKIPAVRN KRVRWLEKEE AKRLIDECPE PLKSVVKFAL ATGLRKSNII NLEWQQIDMQ RRVAWVNPEE SKSNRAIGVA LNDTACKVLR DQIGKHHKWV FVHTKAAKRA DGTSTPAVRK MRIDSKTSWL SACRRAGIED FRFHDLRHTW ASWLIQSGVP LSVLQEMGGW ESIEMVRRYA HLAPNHLTEH ARKIDDIFGD NVPNMSHSEI MEDIKKA
Length	387
Mol. Wt	45.09 kDa
pI	10.1 (calculated)
Extinction coefficient	89,950 - 90,450 (calc based on 5 Y, 15 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	184..356	PF00589 Phage integrase family		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=intD taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128520	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947162	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001842	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P24218	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10507	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10507	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947162	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000500	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0502	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	2.12E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	1.77+/-0.124	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.028374893	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	17	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	11	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	11^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
intD
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:565181..565221 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0537 (EcoliWiki Page)	NCBI GEO profiles for intD
microarray	GenExpDB:b0537 (EcoliWiki Page)	Summary of data for intD from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to intD Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10507	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0502	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0537
EcoGene	EcoGene:EG10507	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000500	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001842	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000047807 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	S0325	From SHIGELLACYC
E. coli O157	INTH	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:56349
Pfam (EcoliWiki Page)	PF00589 Phage integrase family
EcoCyc	EcoCyc:EG10507	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10507	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000500	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0502	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0001842	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

intD:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to intD Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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