ileS:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

ileS

Gene Synonym(s)

ECK0027, b0026, JW0024, ilvS[1], ilvS

Product Desc.

ILES[2][3]

Isoleucine--tRNA ligase[4]

Product Synonyms(s)

isoleucyl-tRNA synthetase[1], B0026[2][1], IlvS[2][1], IleS[2][1] , ECK0027, ilvS, JW0024, b0026

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): ribF-ileS-lspA-fkpB-ispH[2], ileS-lsp, ileS-lspA-lytB, ribF-ileS-lspA-slpA-lytB

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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ileS is an essential gene; the ileS mutants in the Keio collection have a second intact copy of ileS (R. D'Ari and K. Nakahihashi, personal communication cited in Baba, 2000).[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ileS

Mnemonic

Isoleucine

Synonyms

ECK0027, b0026, JW0024, ilvS[1], ilvS

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

0.48 minutes 

MG1655: 22391..25207
<gbrowseImage> name=NC_000913:22391..25207 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 26463..29279
<gbrowseImage> name=NC_012967:26463..29279 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 22391..25207
<gbrowseImage> name=NC_012759:22391..25207 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 22391..25207
<gbrowseImage> name=NC_007779:22391..25207 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 22391..25207
<gbrowseImage> name=NC_010473:22391..25207 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

22394

Edman degradation

PMID:6390679
PMID:7929087


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ileSG94R

G94R

6000-fold increase in Km for isoleucine and 4-fold increase in Km for ATP, with no effect on activity

seeded from UniProt:P00956

ileST243R

T243R

Abolishes pretransfer editing

seeded from UniProt:P00956

ileST246A

T246A

Nearly the same editing activity as the wild-type

seeded from UniProt:P00956

ileST241A

T241A

Nearly the same editing activity as the wild-type

seeded from UniProt:P00956

ileST242A

T242A

Abolishes editing activity against valine, with little change in aminoacylation activity; when associated with A-250

seeded from UniProt:P00956

ileST242P

T242P

Abolishes editing activity against valine, with little change in aminoacylation activity

seeded from UniProt:P00956

ileST243A

T243A

Abolishes editing activity against valine, with little change in aminoacylation activity

seeded from UniProt:P00956

ileSW421A

W421A

Abolishes translocation from the aminoacylation site to the editing site, without effect on aminoacylation activity and posttransfer editing; when associated with A-183

seeded from UniProt:P00956

ileSN250A

N250A

Abolishes editing activity against valine, with little change in aminoacylation activity

seeded from UniProt:P00956

ileSH333A

H333A

Alters the specificity for hydrolysis of the aminoacyl tRNA ester, with no effect on pretransfer editing

seeded from UniProt:P00956

ileSD342A,N

D342A,N

Strong decrease in total editing and deacylation activities. Severely deficient in translocation from the aminoacylation site to the editing site

seeded from UniProt:P00956

ileSD342E

D342E

Reduces 2- to 3-fold the total editing activity and 2-fold the deacylation activity. Moderately reduces translocation from the aminoacylation site to the editing site

seeded from UniProt:P00956

ileSK183A

K183A

Abolishes translocation from the aminoacylation site to the editing site, without effect on aminoacylation activity and posttransfer editing; when associated with A-421

seeded from UniProt:P00956

ileSC97S

C97S

No effect on activity

seeded from UniProt:P00956

ileSF594L

F594L

(in strain: PS102)

Strain variation; seeded from UniProt:P00956

ileSI102N

I102N

No significant effect on activity

seeded from UniProt:P00956

ileS1

PMID:5541530

CGSC:7344


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Notes

The Keio collection[5] lists a deletion of ileS. The insertion in this strain is a duplication of the ileS region.[6] IleS is the only Ile-aminoacyl tRNA synthetase in the genome.


Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0024

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCAGTGACTATAAATCAACCCT

Primer 2:CCGGCAAACTTACGTTTTTCACC

6H3

Kohara Phage

Genobase

PMID:3038334

22B12

Kohara Phage

Genobase

PMID:3038334

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[7]

est. P1 cotransduction: 44% [8]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[7]

est. P1 cotransduction: 71% [8]
Synonyms:car-96::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10492

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10492

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000485

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944761

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0487

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000094

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

IleS

Synonyms

isoleucyl-tRNA synthetase[1], B0026[2][1], IlvS[2][1], IleS[2][1] , ECK0027, ilvS, JW0024, b0026

Product description

ILES[2][3]

Isoleucine--tRNA ligase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001412

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002300

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002301

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009008

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009080

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013155

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010663

F

Seeded from EcoCyc (v14.0)

complete

GO:0004812

aminoacyl-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001412

F

Seeded from EcoCyc (v14.0)

complete

GO:0004812

aminoacyl-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002300

F

Seeded from EcoCyc (v14.0)

complete

GO:0004812

aminoacyl-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009008

F

Seeded from EcoCyc (v14.0)

complete

GO:0004812

aminoacyl-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009080

F

Seeded from EcoCyc (v14.0)

complete

GO:0004812

aminoacyl-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013155

F

Seeded from EcoCyc (v14.0)

complete

GO:0004812

aminoacyl-tRNA ligase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0030

F

Seeded from EcoCyc (v14.0)

complete

GO:0004822

isoleucine-tRNA ligase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_02002

F

Seeded from EcoCyc (v14.0)

complete

GO:0004822

isoleucine-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002301

F

Seeded from EcoCyc (v14.0)

complete

GO:0004822

isoleucine-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015905

F

Seeded from EcoCyc (v14.0)

complete

GO:0004822

isoleucine-tRNA ligase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018353

F

Seeded from EcoCyc (v14.0)

complete

GO:0004822

isoleucine-tRNA ligase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:6.1.1.5

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_02002

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001412

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002300

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002301

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009008

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009080

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013155

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015905

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018353

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_02002

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001412

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002300

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002301

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009008

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009080

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013155

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018353

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001412

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002300

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002301

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009008

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009080

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013155

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015905

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018353

P

Seeded from EcoCyc (v14.0)

complete

GO:0006412

translation

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0648

P

Seeded from EcoCyc (v14.0)

complete

GO:0006418

tRNA aminoacylation for protein translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001412

P

Seeded from EcoCyc (v14.0)

complete

GO:0006418

tRNA aminoacylation for protein translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002300

P

Seeded from EcoCyc (v14.0)

complete

GO:0006418

tRNA aminoacylation for protein translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009008

P

Seeded from EcoCyc (v14.0)

complete

GO:0006418

tRNA aminoacylation for protein translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR009080

P

Seeded from EcoCyc (v14.0)

complete

GO:0006418

tRNA aminoacylation for protein translation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013155

P

Seeded from EcoCyc (v14.0)

complete

GO:0006428

isoleucyl-tRNA aminoacylation

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_02002

P

Seeded from EcoCyc (v14.0)

complete

GO:0006428

isoleucyl-tRNA aminoacylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002301

P

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015905

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018353

F

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0862

F

Seeded from EcoCyc (v14.0)

complete

GO:0016874

ligase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0436

F

Seeded from EcoCyc (v14.0)

complete

GO:0046677

response to antibiotic

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0046

P

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

yccE

PMID:16606699

Experiment(s):EBI-1135335

Protein

dnaJ

PMID:16606699

Experiment(s):EBI-1135335

Protein

glpD

PMID:16606699

Experiment(s):EBI-1135335

Protein

tufA

PMID:15690043

Experiment(s):EBI-889355

Protein

hyaD

PMID:15690043

Experiment(s):EBI-886272

Protein

ppk

PMID:15690043

Experiment(s):EBI-886272

Protein

rbsB

PMID:15690043

Experiment(s):EBI-886272

Protein

allR

PMID:15690043

Experiment(s):EBI-886272

Protein

yjgL

PMID:15690043

Experiment(s):EBI-886272

Protein

allR

PMID:19402753

LCMS(ID Probability):99.6

Protein

tufB

PMID:19402753

MALDI(Z-score):19.837061

Protein

map

PMID:19402753

MALDI(Z-score):38.704436

Protein

ppk

PMID:19402753

LCMS(ID Probability):99.6

Protein

yjgL

PMID:19402753

LCMS(ID Probability):99.6

Protein

rbsB

PMID:19402753

LCMS(ID Probability):99.6

Protein

hyaD

PMID:19402753

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSDYKSTLNL PETGFPMRGD LAKREPGMLA RWTDDDLYGI IRAAKKGKKT FILHDGPPYA
NGSIHIGHSV NKILKDIIVK SKGLSGYDSP YVPGWDCHGL PIELKVEQEY GKPGEKFTAA
EFRAKCREYA ATQVDGQRKD FIRLGVLGDW SHPYLTMDFK TEANIIRALG KIIGNGHLHK
GAKPVHWCVD CRSALAEAEV EYYDKTSPSI DVAFQAVDQD ALKAKFAVSN VNGPISLVIW
TTTPWTLPAN RAISIAPDFD YALVQIDGQA VILAKDLVES VMQRIGVTDY TILGTVKGAE
LELLRFTHPF MGFDVPAILG DHVTLDAGTG AVHTAPGHGP DDYVIGQKYG LETANPVGPD
GTYLPGTYPT LDGVNVFKAN DIVVALLQEK GALLHVEKMQ HSYPCCWRHK TPIIFRATPQ
WFVSMDQKGL RAQSLKEIKG VQWIPDWGQA RIESMVANRP DWCISRQRTW GVPMSLFVHK
DTEELHPRTL ELMEEVAKRV EVDGIQAWWD LDAKEILGDE ADQYVKVPDT LDVWFDSGST
HSSVVDVRPE FAGHAADMYL EGSDQHRGWF MSSLMISTAM KGKAPYRQVL THGFTVDGQG
RKMSKSIGNT VSPQDVMNKL GADILRLWVA STDYTGEMAV SDEILKRAAD SYRRIRNTAR
FLLANLNGFD PAKDMVKPEE MVVLDRWAVG CAKAAQEDIL KAYEAYDFHE VVQRLMRFCS
VEMGSFYLDI IKDRQYTAKA DSVARRSCQT ALYHIAEALV RWMAPILSFT ADEVWGYLPG
EREKYVFTGE WYEGLFGLAD SEAMNDAFWD ELLKVRGEVN KVIEQARADK KVGGSLEAAV
TLYAEPELSA KLTALGDELR FVLLTSGATV ADYNDAPADA QQSEVLKGLK VALSKAEGEK
CPRCWHYTQD VGKVAEHAEI CGRCVSNVAG DGEKRKFA
Length

938

Mol. Wt

104.297 kDa

pI

5.8 (calculated)

Extinction coefficient

175,670 - 177,420 (calc based on 33 Y, 23 W, and 14 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P00956

Domain

27..641

PF00133 tRNA synthetases class I (I, L, M and V)

PMID:19920124

Domain

898..927

PF06827 Zinc finger found in FPG and IleRS

PMID:19920124

Domain

685..842

PF08264 Anticodon-binding domain

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ileS taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128020

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000094

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P00956

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10492

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10492

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944761

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000485

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944761

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0487

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

2.15E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

64.061+/-0.467

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.183238636

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

6143

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

2270

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

4760

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

ribF-ileS-lspA-fkpB-ispH

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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:22371..22411 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0026 (EcoliWiki Page)

NCBI GEO profiles for ileS

microarray

GenExpDB:b0026 (EcoliWiki Page)

Summary of data for ileS from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to ileS Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10492

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0487

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0026

EcoGene

EcoGene:EG10492

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000485

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000094

Escherichia coli str. K-12 substr. MG1655

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<protect></protect>

Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000016716 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000032851 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT5G49030 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000016704 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00033207 (score: 1.000; bootstrap: 94%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00002153 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000016236 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000005065 (score: 1.000; bootstrap: 71%)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-030616-608 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0231256 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0036569 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA18866-PA (score: 1.000; bootstrap: 96%)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000015565 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000303279 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000010225 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000005505 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Mus musculus

  • MGI:1919586 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Oryza gramene

  • Q6ZGC2 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000003348 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000003222 (score: 1.000; bootstrap: 51%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YPL040C (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPCC18B58c (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000138839 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00009778001 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000051670 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

ILES

From SHIGELLACYC

E. coli O157

ILES

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:47323

Superfamily (EcoliWiki Page)

SUPERFAMILY:50677

Superfamily (EcoliWiki Page)

SUPERFAMILY:52374

Panther (EcoliWiki Page)

PTHR11946:SF9

Pfam (EcoliWiki Page)

PF00133 tRNA synthetases class I (I, L, M and V)

Pfam (EcoliWiki Page)

PF06827 Zinc finger found in FPG and IleRS

Pfam (EcoliWiki Page)

PF08264 Anticodon-binding domain

EcoCyc

EcoCyc:EG10492

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10492

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000485

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0487

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000094

Escherichia coli str. K-12 substr. MG1655

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<protect></protect>

Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. ↑ Yamamoto, N et al. (2009) Update on the Keio collection of Escherichia coli single-gene deletion mutants. Mol. Syst. Biol. 5 335 PubMed
  7. ↑ 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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