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Gene |

Product(s) |

Expression|

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Standard Name	hyaB
Gene Synonym(s)	ECK0964, b0973, JW0955^[1], JW0955
Product Desc.	hydrogenase 1, large subunit^[2]^[3]; Component of hydrogenase 1^[2]^[3] Hydrogenase 1 large subunit [NiFe], periplasmic^[4]
Product Synonyms(s)	hydrogenase 1, large subunit^[1], B0973^[2]^[1], HyaB^[2]^[1] , ECK0964, JW0955, b0973
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): hyaABCDEF^[2]
Regulation/Activity
Quick Links
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Notes

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HyaB is co-exported bound to TAT substrate HyaA. HT_Cmplx26_Mem: HyaB+HybO.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hyaB
Mnemonic	Hydrogenase 1
Synonyms	ECK0964, b0973, JW0955^[1], JW0955
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	22.25 minutes	MG1655: 1032477..1034270 <gbrowseImage> name=NC_000913:1032477..1034270 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1050345..1052138 <gbrowseImage> name=NC_012967:1050345..1052138 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 935445..937238 <gbrowseImage> name=NC_012759:935445..937238 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1033676..1035469 <gbrowseImage> name=NC_007779:1033676..1035469 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1086405..1088198 <gbrowseImage> name=NC_010473:1086405..1088198 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1032477	Edman degradation	PMID:2180913
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔhyaB (Keio:JW0955)	deletion	deletion	PMID:16738554	Shigen CGSC8951^[5]
hyaB::Tn5KAN-2 (FB20075)	Insertion at nt 1627 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20075	does not contain pKD46
ΔhyaB787::kan			PMID:16738554	CGSC:101446
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW0955	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGCACTCAGTACGAAACTCA Primer 2:CCACGCACCTGCACGGAGATCAG
4H9	Kohara Phage	Genobase	PMID:3038334
4H11	Kohara Phage	Genobase	PMID:3038334
zcb-3059::Tn10	Linked marker	CAG12094 = CGSC7358^[5]	est. P1 cotransduction: 31% ^[6]
zcc-282::Tn10	Linked marker	CAG18466 = CGSC7359^[5]	est. P1 cotransduction: 31% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10469	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10469	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000462	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945580	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0464	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003288	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	HyaB
Synonyms	hydrogenase 1, large subunit^[1], B0973^[2]^[1], HyaB^[2]^[1] , ECK0964, JW0955, b0973
Product description	hydrogenase 1, large subunit^[2]^[3]; Component of hydrogenase 1^[2]^[3] Hydrogenase 1 large subunit [NiFe], periplasmic^[4]
EC number (for enzymes)	1.12.99.6^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0030288	outer membrane-bounded periplasmic space				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008901	ferredoxin hydrogenase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001501	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008901	ferredoxin hydrogenase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018194	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	PMID:16858726	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0016151	nickel ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001501	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016151	nickel ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018194	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016151	nickel ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0533	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016491	oxidoreductase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	F	Seeded from EcoCyc (v14.0)	complete
	GO:0019898	extrinsic to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9903	C	Seeded from EcoCyc (v14.0)	complete
	GO:0033748	hydrogenase (acceptor) activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:1.12.99.6	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001501	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018194	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of hydrogenase 1	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
membrane
Periplasm	PMID:2180913	EchoLocation:hyaB
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSTQYETQGY TINNAGRRLV VDPITRIEGH MRCEVNINDQ NVITNAVSCG TMFRGLEIIL QGRDPRDAWA FVERICGVCT GVHALASVYA IEDAIGIKVP DNANIIRNIM LATLWCHDHL VHFYQLAGMD WIDVLDALKA DPRKTSELAQ SLSSWPKSSP GYFFDVQNRL KKFVEGGQLG IFRNGYWGHP QYKLPPEANL MGFAHYLEAL DFQREIVKIH AVFGGKNPHP NWIVGGMPCA INIDESGAVG AVNMERLNLV QSIITRTADF INNVMIPDAL AIGQFNKPWS EIGTGLSDKC VLSYGAFPDI ANDFGEKSLL MPGGAVINGD FNNVLPVDLV DPQQVQEFVD HAWYRYPNDQ VGRHPFDGIT DPWYNPGDVK GSDTNIQQLN EQERYSWIKA PRWRGNAMEV GPLARTLIAY HKGDAATVES VDRMMSALNL PLSGIQSTLG RILCRAHEAQ WAAGKLQYFF DKLMTNLKNG NLATASTEKW EPATWPTECR GVGFTEAPRG ALGHWAAIRD GKIDLYQCVV PTTWNASPRD PKGQIGAYEA ALMNTKMAIP EQPLEILRTL HSFDPCLACS THVLGDDGSE LISVQVR
Length	597
Mol. Wt	66.253 kDa
pI	5.9 (calculated)
Extinction coefficient	113,330 - 114,830 (calc based on 17 Y, 16 W, and 12 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	54..582	PF00374 Nickel-dependent hydrogenase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hyaB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16128939	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945580	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003288	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ACD8	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10469	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10469	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945580	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000462	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0464	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	1^a	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
Protein	E. coli K-12 MG1655	7	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	0^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
hyaABCDEF
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1032457..1032497 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b0973 (EcoliWiki Page)	NCBI GEO profiles for hyaB
microarray	GenExpDB:b0973 (EcoliWiki Page)	Summary of data for hyaB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to hyaB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10469	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0464	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b0973
EcoGene	EcoGene:EG10469	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000462	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003288	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	HYAB	From SHIGELLACYC
E. coli O157	HYAB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00374 Nickel-dependent hydrogenase
Superfamily (EcoliWiki Page)	SUPERFAMILY:56762
EcoCyc	EcoCyc:EG10469	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10469	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000462	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0464	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003288	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

hyaB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hyaB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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