hslV:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

hslV

Gene Synonym(s)

ECK3924, b3932, JW3903, htpO, clpQ, yiiC[1], yiiC

Product Desc.

peptidase component of the HslVU protease[2][3];

Component of HslV hexamer[3]; HslVU protease[3]

Heat-inducible ATP-dependent protease HslVU, protease subunit; involved in the degradation of misfolded proteins[4]

Product Synonyms(s)

peptidase component of the HslUV protease[1], B3932[2][1], ClpQ[2][1], YiiC[2][1], HtpO[2][1], HslV[2][1] , clpQ, ECK3924, htpO, JW3903, yiiC, b3932

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): hslVU[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hslV

Mnemonic

heat shock locus Heat-shock locus

Synonyms

ECK3924, b3932, JW3903, htpO, clpQ, yiiC[1], yiiC

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

88.79 minutes 

MG1655: 4120310..4119780
<gbrowseImage> name=NC_000913:4119780..4120310 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4101770..4101240
<gbrowseImage> name=NC_012967:4101240..4101770 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4009449..4009979
<gbrowseImage> name=NC_012759:4009449..4009979 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3514394..3514924
<gbrowseImage> name=NC_007779:3514394..3514924 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4220007..4219477
<gbrowseImage> name=NC_010473:4219477..4220007 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4119783

Edman degradation

PMID:8650174


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔhslV (Keio:JW3903)

deletion

deletion

PMID:16738554

Shigen
CGSC10818[5]

ΔhslV720::kan

PMID:16738554

CGSC:101424


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3903

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCACAACTATAGTAAGCGTACG

Primer 2:CCCGCTTTGTAGCTTAATTCTTC

12E3

Kohara Phage

Genobase

PMID:3038334

4H12

Kohara Phage

Genobase

PMID:3038334

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[5]

est. P1 cotransduction: 7% [6]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[5]

est. P1 cotransduction: 42% [6]
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11676

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11676

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001619

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948429

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1627

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012845

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

HslV

Synonyms

peptidase component of the HslUV protease[1], B3932[2][1], ClpQ[2][1], YiiC[2][1], HtpO[2][1], HslV[2][1] , clpQ, ECK3924, htpO, JW3903, yiiC, b3932

Product description

peptidase component of the HslVU protease[2][3];

Component of HslV hexamer[3]; HslVU protease[3]

Heat-inducible ATP-dependent protease HslVU, protease subunit; involved in the degradation of misfolded proteins[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0004298

threonine-type endopeptidase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001353

F

Seeded from EcoCyc (v14.0)

complete

GO:0004298

threonine-type endopeptidase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0888

F

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0005839

proteasome core complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001353

C

Seeded from EcoCyc (v14.0)

complete

GO:0006950

response to stress

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0346

P

Seeded from EcoCyc (v14.0)

complete

GO:0008233

peptidase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00248

F

Seeded from EcoCyc (v14.0)

complete

GO:0008233

peptidase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0645

F

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0009408

response to heat

PMID:8349564

IEP: Inferred from Expression Pattern

P

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of HslV hexamer

could be indirect

Protein

deaD

PMID:15690043

Experiment(s):EBI-880750

Protein

hslU

PMID:15690043

Experiment(s):EBI-880750, EBI-886932

Protein

rplB

PMID:15690043

Experiment(s):EBI-880750

Protein

tpiA

PMID:16606699

Experiment(s):EBI-1147088

Protein

rplC

PMID:15690043

Experiment(s):EBI-880750, EBI-886859

Protein

yggN

PMID:16606699

Experiment(s):EBI-1147088

Protein

rplD

PMID:15690043

Experiment(s):EBI-880750, EBI-886859

Protein

yghT

PMID:16606699

Experiment(s):EBI-1147088

Protein

rplI

PMID:15690043

Experiment(s):EBI-880750

Protein

prs

PMID:16606699

Experiment(s):EBI-1147088

Protein

rplJ

PMID:15690043

Experiment(s):EBI-880750

Protein

rplQ

PMID:16606699

Experiment(s):EBI-1147088

Protein

rplS

PMID:15690043

Experiment(s):EBI-880750

Protein

rplV

PMID:15690043

Experiment(s):EBI-880750, EBI-886859

Protein

rpsG

PMID:15690043

Experiment(s):EBI-880750

Protein

rpsM

PMID:15690043

Experiment(s):EBI-880750

Protein

tufA

PMID:15690043

Experiment(s):EBI-880750

Protein

rluB

PMID:15690043

Experiment(s):EBI-880750

Protein

accA

PMID:15690043

Experiment(s):EBI-886859

Protein

accB

PMID:15690043

Experiment(s):EBI-886859

Protein

accC

PMID:15690043

Experiment(s):EBI-886859

Protein

accD

PMID:15690043

Experiment(s):EBI-886859

Protein

ccmB

PMID:15690043

Experiment(s):EBI-886859

Protein

gabP

PMID:15690043

Experiment(s):EBI-886859

Protein

rplL

PMID:15690043

Experiment(s):EBI-886859

Protein

rplU

PMID:15690043

Experiment(s):EBI-886859

Protein

rplX

PMID:15690043

Experiment(s):EBI-886859

Protein

rpmC

PMID:15690043

Experiment(s):EBI-886859

Protein

rpmG

PMID:15690043

Experiment(s):EBI-886859

Protein

rpsP

PMID:15690043

Experiment(s):EBI-886859

Protein

yihI

PMID:15690043

Experiment(s):EBI-886859

Protein

yhjD

PMID:15690043

Experiment(s):EBI-886859

Protein

rplS

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

Protein

yhjD

PMID:19402753

LCMS(ID Probability):99.0

Protein

lon

PMID:19402753

MALDI(Z-score):28.228419

Protein

yihI

PMID:19402753

LCMS(ID Probability):99.0

Protein

rplD

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

Protein

accB

PMID:19402753

LCMS(ID Probability):99.0

Protein

gabP

PMID:19402753

LCMS(ID Probability):99.0

Protein

Subunits of HslVU protease

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MTTIVSVRRN GHVVIAGDGQ ATLGNTVMKG NVKKVRRLYN DKVIAGFAGG TADAFTLFEL
FERKLEMHQG HLVKAAVELA KDWRTDRMLR KLEALLAVAD ETASLIITGN GDVVQPENDL
IAIGSGGPYA QAAARALLEN TELSAREIAE KALDIAGDIC IYTNHFHTIE ELSYKA
Length

176

Mol. Wt

19.092 kDa

pI

6.4 (calculated)

Extinction coefficient

11,460 - 11,585 (calc based on 4 Y, 1 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed (Probable)

UniProt:P0A7B8

Domain

2..172

PF00227 Proteasome subunit

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hslV taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131770

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948429

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012845

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A7B8

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11676

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11676

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948429

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001619

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1627

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

6.13E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

6354

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1016

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

4213

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

hslVU

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4120290..4120330 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3932 (EcoliWiki Page)

NCBI GEO profiles for hslV

microarray

GenExpDB:b3932 (EcoliWiki Page)

Summary of data for hslV from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

GFP Fusion

Intergenic region (4119788..4120017) fused to gfpmut2.

OpenBioSystems

GFP fusion described in Zaslaver, et al.
Plate:109-AZ04; Well:E2[7]

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Notes

Accessions Related to hslV Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11676

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1627

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3932

EcoGene

EcoGene:EG11676

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001619

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012845

Escherichia coli str. K-12 substr. MG1655

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Notes

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Dictyostelium discoideum

  • DDB0190148 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

HSLV

From SHIGELLACYC

E. coli O157

HSLV

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00227 Proteasome subunit

Panther (EcoliWiki Page)

PTHR11599:SF38

Superfamily (EcoliWiki Page)

SUPERFAMILY:56235

EcoCyc

EcoCyc:EG11676

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11676

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001619

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1627

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012845

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. ↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

Categories

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