hslU:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

hslU

Gene Synonym(s)

ECK3923, b3931, JW3902, clpY, htpI[1], htpI

Product Desc.

ATPase component of the HslVU protease[2][3];

Component of HslU hexamer[3]; HslVU protease[3]

Heat-inducible ATP-dependent protease HslVU, ATPase subunit; involved in the degradation of misfolded proteins; heat shock protein D48.5[4]

Product Synonyms(s)

molecular chaperone and ATPase component of HslUV protease[1], B3931[2][1], HtpI[2][1], ClpY[2][1], HslU[2][1], D48.5[2][1] , clpY, ECK3923, htpI, JW3902, b3931

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): hslVU[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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An hslU deletion suppresses dnaA46(Ts). Binds TrxA (Kumar, 2004).[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hslU

Mnemonic

heat shock locus Heat shock locus

Synonyms

ECK3923, b3931, JW3902, clpY, htpI[1], htpI

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

88.77 minutes 

MG1655: 4119770..4118439
<gbrowseImage> name=NC_000913:4118439..4119770 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4101230..4099899
<gbrowseImage> name=NC_012967:4099899..4101230 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4008108..4009439
<gbrowseImage> name=NC_012759:4008108..4009439 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3514934..3516265
<gbrowseImage> name=NC_007779:3514934..3516265 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4219467..4218136
<gbrowseImage> name=NC_010473:4218136..4219467 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔhslU (Keio:JW3902)

deletion

deletion

PMID:16738554

Shigen
CGSC10817[5]

ΔhslU::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938

ΔhslU790::kan

PMID:16738554

CGSC:101422


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3902

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCTCTGAAATGACCCCACGCGA

Primer 2:CCTAGGATAAAACGGCTCAGATC

12E3

Kohara Phage

Genobase

PMID:3038334

4H12

Kohara Phage

Genobase

PMID:3038334

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[5]

est. P1 cotransduction: 7% [6]

pflD501::Tn10

Linked marker

CAG18477 = CGSC7470[5]

est. P1 cotransduction: 39% [6]
Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11881

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11881

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001811

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948430

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1827

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012843

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

HslU

Synonyms

molecular chaperone and ATPase component of HslUV protease[1], B3931[2][1], HtpI[2][1], ClpY[2][1], HslU[2][1], D48.5[2][1] , clpY, ECK3923, htpI, JW3902, b3931

Product description

ATPase component of the HslVU protease[2][3];

Component of HslU hexamer[3]; HslVU protease[3]

Heat-inducible ATP-dependent protease HslVU, ATPase subunit; involved in the degradation of misfolded proteins; heat shock protein D48.5[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004491

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00249

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003959

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004491

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013093

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00249

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004491

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006950

response to stress

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0346

P

Seeded from EcoCyc (v14.0)

complete

GO:0008233

peptidase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00249

F

Seeded from EcoCyc (v14.0)

complete

GO:0009376

HslUV protease complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004491

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

PMID:16858726

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0016887

ATPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004491

F

Seeded from EcoCyc (v14.0)

complete

GO:0009408

response to heat

PMID:8349564

IEP: Inferred from Expression Pattern

P

complete

GO:0017111

nucleoside-triphosphatase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0070011

peptidase activity, acting on L-amino acid peptides

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004491

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of HslU hexamer

could be indirect

Protein

cca

PMID:15690043

Experiment(s):EBI-880782

Protein

cysW

PMID:15690043

Experiment(s):EBI-880782

Protein

dnaJ

PMID:15690043

Experiment(s):EBI-880782, EBI-886932

Protein

dnaK

PMID:15690043

Experiment(s):EBI-880782, EBI-886932

Protein

ftsZ

PMID:15690043

Experiment(s):EBI-880782

Protein

fusA

PMID:15690043

Experiment(s):EBI-880782

Protein

gatZ

PMID:15690043

Experiment(s):EBI-880782

Protein

gpmB

PMID:15690043

Experiment(s):EBI-880782

Protein

lon

PMID:15690043

Experiment(s):EBI-880782

Protein

metE

PMID:15690043

Experiment(s):EBI-880782

Protein

metK

PMID:15690043

Experiment(s):EBI-880782

Protein

mreB

PMID:15690043

Experiment(s):EBI-880782

Protein

narG

PMID:15690043

Experiment(s):EBI-880782

Protein

nuoC

PMID:15690043

Experiment(s):EBI-880782

Protein

pstB

PMID:15690043

Experiment(s):EBI-880782

Protein

recA

PMID:15690043

Experiment(s):EBI-880782

Protein

rplJ

PMID:15690043

Experiment(s):EBI-880782

Protein

secA

PMID:15690043

Experiment(s):EBI-880782

Protein

tufA

PMID:15690043

Experiment(s):EBI-880782, EBI-886932

Protein

ygeG

PMID:15690043

Experiment(s):EBI-880782

Protein

accB

PMID:15690043

Experiment(s):EBI-886932

Protein

accC

PMID:15690043

Experiment(s):EBI-886932

Protein

accD

PMID:15690043

Experiment(s):EBI-886932

Protein

ydeN

PMID:15690043

Experiment(s):EBI-886932

Protein

ccmB

PMID:15690043

Experiment(s):EBI-886932

Protein

iciA

PMID:15690043

Experiment(s):EBI-886932

Protein

mglB

PMID:15690043

Experiment(s):EBI-886932

Protein

ompX

PMID:15690043

Experiment(s):EBI-886932

Protein

ribD

PMID:15690043

Experiment(s):EBI-886932

Protein

rplC

PMID:15690043

Experiment(s):EBI-886932

Protein

rplD

PMID:15690043

Experiment(s):EBI-886932

Protein

rplL

PMID:15690043

Experiment(s):EBI-886932

Protein

rplM

PMID:15690043

Experiment(s):EBI-886932

Protein

rplP

PMID:15690043

Experiment(s):EBI-886932

Protein

rpsB

PMID:15690043

Experiment(s):EBI-886932

Protein

rpsE

PMID:15690043

Experiment(s):EBI-886932

Protein

rpsG

PMID:15690043

Experiment(s):EBI-886932

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-886932

Protein

thrA

PMID:15690043

Experiment(s):EBI-886932

Protein

ydgA

PMID:15690043

Experiment(s):EBI-886932

Protein

rpsJ

PMID:19402753

LCMS(ID Probability):99.6

Protein

fusA

PMID:19402753

MALDI(Z-score):24.085201

Protein

ribD

PMID:19402753

LCMS(ID Probability):99.0

Protein

recA

PMID:19402753

MALDI(Z-score):32.936622

Protein

metE

PMID:19402753

MALDI(Z-score):25.787725

Protein

hslV

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):17.053160

Protein

metK

PMID:19402753

MALDI(Z-score):33.658153

Protein

ompX

PMID:19402753

LCMS(ID Probability):99.0

Protein

tufB

PMID:19402753

MALDI(Z-score):20.769725

Protein

dnaJ

PMID:19402753

LCMS(ID Probability):99.6 MALDI(Z-score):17.319184

Protein

mreB

PMID:19402753

MALDI(Z-score):36.427134

Protein

gatZ

PMID:19402753

MALDI(Z-score):25.337942

Protein

ydgA

PMID:19402753

LCMS(ID Probability):99.6

Protein

argP

PMID:19402753

LCMS(ID Probability):99.6

Protein

thrA

PMID:19402753

LCMS(ID Probability):99.6

Protein

mglB

PMID:19402753

LCMS(ID Probability):99.6

Protein

accB

PMID:19402753

LCMS(ID Probability):99.0

Protein

ydeN

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplL

PMID:19402753

LCMS(ID Probability):99.6

Protein

Subunits of HslVU protease

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSEMTPREIV SELDKHIIGQ DNAKRSVAIA LRNRWRRMQL NEELRHEVTP KNILMIGPTG
VGKTEIARRL AKLANAPFIK VEATKFTEVG YVGKEVDSII RDLTDAAVKM VRVQAIEKNR
YRAEELAEER ILDVLIPPAK NNWGQTEQQQ EPSAARQAFR KKLREGQLDD KEIEIDLAAA
PMGVEIMAPP GMEEMTSQLQ SMFQNLGGQK QKARKLKIKD AMKLLIEEEA AKLVNPEELK
QDAIDAVEQH GIVFIDEIDK ICKRGESSGP DVSREGVQRD LLPLVEGCTV STKHGMVKTD
HILFIASGAF QIAKPSDLIP ELQGRLPIRV ELQALTTSDF ERILTEPNAS ITVQYKALMA
TEGVNIEFTD SGIKRIAEAA WQVNESTENI GARRLHTVLE RLMEEISYDA SDLSGQNITI
DADYVSKHLD ALVADEDLSR FIL
Length

443

Mol. Wt

49.594 kDa

pI

5.2 (calculated)

Extinction coefficient

23,950 - 24,200 (calc based on 5 Y, 3 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

53..142

PF00004 ATPase family associated with various cellular activities (AAA)

PMID:19920124

Domain

170..329

PF07724 AAA domain (Cdc48 subfamily)

PMID:19920124

Domain

335..429

PF10431 C-terminal, D2-small domain, of ClpB protein

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hslU taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131769

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948430

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012843

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6H5

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11881

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11881

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948430

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001811

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1827

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.04E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

150.698+/-0.827

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.17059+/-0.01085

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by FISH

PMID:20671182

mRNA

Ecoli K-12

0.077385424

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

9329

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

1426

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

6438

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

hslVU

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4119750..4119790 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3931 (EcoliWiki Page)

NCBI GEO profiles for hslU

microarray

GenExpDB:b3931 (EcoliWiki Page)

Summary of data for hslU from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to hslU Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11881

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1827

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3931

EcoGene

EcoGene:EG11881

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001811

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012843

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Dictyostelium discoideum

  • DDB0188012 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000042527 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

HSLU

From SHIGELLACYC

E. coli O157

HSLU

From ECOO157CYC

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00004 ATPase family associated with various cellular activities (AAA)

Superfamily (EcoliWiki Page)

SUPERFAMILY:52540

Panther (EcoliWiki Page)

PTHR11262:SF3

Pfam (EcoliWiki Page)

PF07724 AAA domain (Cdc48 subfamily)

Pfam (EcoliWiki Page)

PF10431 C-terminal, D2-small domain, of ClpB protein

EcoCyc

EcoCyc:EG11881

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11881

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001811

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1827

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012843

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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