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Standard Name	hscB
Gene Synonym(s)	ECK2524, b2527, JW2511, yfhE, hsc20^[1]^[2]
Product Desc.	Hsc20 co-chaperone that acts with Hsc66 in IscU iron-sulfur cluster assembly^[2]^[3] Hsc20, DnaJ-like co-chaperone for HscA; specific for IscU^[4]
Product Synonyms(s)	DnaJ-like molecular chaperone specific for IscU^[1], B2527^[2]^[1], YfhE^[2]^[1], HscB^[2]^[1], Hsc20^[2]^[1] , ECK2524, JW2511, yfhE, b2527
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): hscBA-fdx^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hscB
Mnemonic	Heat shock cognate
Synonyms	ECK2524, b2527, JW2511, yfhE, hsc20^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	57.27 minutes	MG1655: 2657489..2656974 <gbrowseImage> name=NC_000913:2656974..2657489 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2580166..2579651 <gbrowseImage> name=NC_012967:2579651..2580166 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2542779..2543294 <gbrowseImage> name=NC_012759:2542779..2543294 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2658123..2657608 <gbrowseImage> name=NC_007779:2657608..2658123 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2749254..2748739 <gbrowseImage> name=NC_010473:2748739..2749254 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
hscB(del) (Keio:JW2511)	deletion	deletion			PMID:16738554	Shigen CGSC9999^[5]
hscB773(del)::kan					PMID:16738554	CGSC:101410
hscB773(del)::FRT	Deletion		Mutagenesis Rate	Decrease in stress induced mutagenesis (SIM).	PMID:23224554	Parent Strain: SMR4562 Experimental Strain: SMR12307	The mutation conferred a significant decrease in SIM with mutant frequency reduced by 68 to 89 percent. See table S3 for full experimental results.
hscB773(del)::FRT	Deletion		Sensitivity to	UV sensitivity	PMID:23224554	Parent Strain: SMR4562 Experimental Strain: SMR12307	The mutation conferred an increase in UV sensitivity. See tables S7 and S1 for a summary of experimental results.
SMR4562 yiaG-yfp FRTcatFRT hscB773(del)::FRTKanFRT	deletion		Sigma ^S activity	Decrease in Sigma^S activity	PMID:23224554	Parental Strain: SMR10582 Experimental Strain: SMR14275	See Table S8 for experimental results.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2511	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGATTACTTCACCCTCTTTGG Primer 2:CCAAAATCGAGCAGTTTTTCTTC
5E10	Kohara Phage	Genobase	PMID:3038334
purC80::Tn10	Linked marker	CAG18470 = CGSC7413^[5]	est. P1 cotransduction: 3% ^[6]
zfh-208::Tn10	Linked marker	CAG18481 = CGSC7417^[5]	est. P1 cotransduction: 81% ^[6] Synonyms:zff-208::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12131	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12131	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002033	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946995	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2052	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008317	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	HscB
Synonyms	DnaJ-like molecular chaperone specific for IscU^[1], B2527^[2]^[1], YfhE^[2]^[1], HscB^[2]^[1], Hsc20^[2]^[1] , ECK2524, JW2511, yfhE, b2527
Product description	Hsc20 co-chaperone that acts with Hsc66 in IscU iron-sulfur cluster assembly^[2]^[3] Hsc20, DnaJ-like co-chaperone for HscA; specific for IscU^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	clpB	PMID:15690043	Experiment(s):EBI-889252
Protein	rpoC	PMID:15690043	Experiment(s):EBI-889252
Protein	tufA	PMID:15690043	Experiment(s):EBI-889252, EBI-894305
Protein	htpG	PMID:15690043	Experiment(s):EBI-894305
Protein	rplA	PMID:15690043	Experiment(s):EBI-894305
Protein	rplJ	PMID:15690043	Experiment(s):EBI-894305
Protein	rplK	PMID:15690043	Experiment(s):EBI-894305
Protein	rplL	PMID:15690043	Experiment(s):EBI-894305
Protein	rpmC	PMID:15690043	Experiment(s):EBI-894305
Protein	rpmG	PMID:15690043	Experiment(s):EBI-894305
Protein	rpsB	PMID:15690043	Experiment(s):EBI-894305
Protein	rpsG	PMID:15690043	Experiment(s):EBI-894305
Protein	rpsN	PMID:15690043	Experiment(s):EBI-894305
Protein	ugpB	PMID:15690043	Experiment(s):EBI-894305
Protein	dcuD	PMID:15690043	Experiment(s):EBI-894305
Protein	pepD	PMID:16606699	Experiment(s):EBI-1143149
Protein	tufB	PMID:19402753	MALDI(Z-score):20.192411
Protein	metK	PMID:19402753	MALDI(Z-score):18.535481
Protein	htpG	PMID:19402753	LCMS(ID Probability):99.0 MALDI(Z-score):28.837684
Protein	clpB	PMID:19402753	MALDI(Z-score):28.627041
Protein	clpX	PMID:19402753	MALDI(Z-score):39.634700
Protein	dcuD	PMID:19402753	LCMS(ID Probability):99.0
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MDYFTLFGLP ARYQLDTQAL SLRFQDLQRQ YHPDKFASGS QAEQLAAVQQ SATINQAWQT LRHPLMRAEY LLSLHGFDLA SEQHTVRDTA FLMEQLELRE ELDEIEQAKD EARLESFIKR VKKMFDTRHQ LMVEQLDNET WDAAADTVRK LRFLDKLRSS AEQLEEKLLD F
Length	171
Mol. Wt	20.137 kDa
pI	4.9 (calculated)
Extinction coefficient	16,960 (calc based on 4 Y, 2 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	References
Domain	2..71	PF00226 DnaJ domain	PMID:19920124
Domain	86..163	PF07743 HSCB C-terminal oligomerisation domain	PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hscB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130452	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946995	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008317	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A6L9	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12131	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12131	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946995	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002033	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2052	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	1.04E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	1461	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	416	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	1151	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
hscBA-fdx
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2657469..2657509 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2527 (EcoliWiki Page)	NCBI GEO profiles for hscB
microarray	GenExpDB:b2527 (EcoliWiki Page)	Summary of data for hscB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2657411..2657643) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ13; Well:E12^[7]
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Notes

Accessions Related to hscB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12131	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2052	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2527
EcoGene	EcoGene:EG12131	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002033	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008317	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000017675 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT5G06410 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000020063 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00034642 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00001033 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000017552 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0215234 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000009138 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000216027 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000010435 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:2141135 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000024461 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YGL018C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC1448 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000155266 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00029026001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	YFHE	From SHIGELLACYC
E. coli O157	YFHE	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00226 DnaJ domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:46565
Superfamily (EcoliWiki Page)	SUPERFAMILY:47144
Panther (EcoliWiki Page)	PTHR14021:SF10
Pfam (EcoliWiki Page)	PF07743 HSCB C-terminal oligomerisation domain
EcoCyc	EcoCyc:EG12131	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12131	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002033	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2052	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008317	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

hscB:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

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Alleles and Phenotypes

Notes

Genetic Interactions

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Genetic Resources

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Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hscB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

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References

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