hscA:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

hscA

Gene Synonym(s)

ECK2523, b2526, JW2510, hsc, hsc66[1][2]

Product Desc.

chaperone, member of Hsp70 protein family[2][3]

Hsc66, DnaK-like chaperone, specific for IscU; involved in FtsZ-ring formation; HscB is the J-like co-chaperone for HscA[4]

Product Synonyms(s)

DnaK-like molecular chaperone specific for IscU[1], B2526[2][1], Hsc[2][1], HscA[2][1], Hsc66[2][1] , ECK2523, hsc, JW2510, b2526

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): hscBA-fdx[2], hscA-fdx[2], hsc-fdx

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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dnaK, hscA, hscC and yegD are paralogs.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

hscA

Mnemonic

Heat shock cognate

Synonyms

ECK2523, b2526, JW2510, hsc, hsc66[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

57.23 minutes 

MG1655: 2656957..2655107
<gbrowseImage> name=NC_000913:2655107..2656957 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2579634..2577784
<gbrowseImage> name=NC_012967:2577784..2579634 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2540912..2542762
<gbrowseImage> name=NC_012759:2540912..2542762 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2657591..2655741
<gbrowseImage> name=NC_007779:2655741..2657591 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2748722..2746872
<gbrowseImage> name=NC_010473:2746872..2748722 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔhscA (Keio:JW2510)

deletion

deletion

PMID:16738554

Shigen
CGSC9998[5]

hscAS216F

S216F

(in hsca1)

Strain variation; seeded from UniProt:P0A6Z1

ΔhscA772::kan

PMID:16738554

CGSC:101408

hscA

deletion

Sensitivity to

increases sensitivity to bicyclomycin

PMID:21357484

fig 2

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2510

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCCTTATTACAAATTAGTGA

Primer 2:CCAACCTCGTCCACGGAATGGC

7F8

Kohara Phage

Genobase

PMID:3038334

5E10

Kohara Phage

Genobase

PMID:3038334

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[5]

est. P1 cotransduction: 4% [6]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[5]

est. P1 cotransduction: 76% [6]
Synonyms:zff-208::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12130

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12130

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002032

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944885

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2051

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008315

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

HscA

Synonyms

DnaK-like molecular chaperone specific for IscU[1], B2526[2][1], Hsc[2][1], HscA[2][1], Hsc66[2][1] , ECK2523, hsc, JW2510, b2526

Product description

chaperone, member of Hsp70 protein family[2][3]

Hsc66, DnaK-like chaperone, specific for IscU; involved in FtsZ-ring formation; HscB is the J-like co-chaperone for HscA[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005515

protein binding

PMID:11983079

IPI: Inferred from Physical Interaction

UniProtKB:P0ACD4
UniProtKB:P0A6L9
UniProtKB:P0A9R9

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:12871959

IPI: Inferred from Physical Interaction

UniProtKB:P0ACD4

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:15485839

IPI: Inferred from Physical Interaction

UniProtKB:P0A6L9

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:19821612

IPI: Inferred from Physical Interaction

EcoCyc:BIOTIN-SYN-CPLX

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001023

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010236

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

PMID:10713091

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

PMID:11323718

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0006457

protein folding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00679

P

Seeded from EcoCyc (v14.0)

complete

GO:0006457

protein folding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010236

P

Seeded from EcoCyc (v14.0)

complete

GO:0016226

iron-sulfur cluster assembly

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010236

P

Seeded from EcoCyc (v14.0)

complete

GO:0016226

iron-sulfur cluster assembly

PMID:10393315

IGI: Inferred from Genetic Interaction

P

Seeded from EcoCyc (v14.0)

Missing: with/from

GO:0016226

iron-sulfur cluster assembly

PMID:10544286

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0016887

ATPase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00679

F

Seeded from EcoCyc (v14.0)

complete

GO:0016887

ATPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010236

F

Seeded from EcoCyc (v14.0)

complete

GO:0016887

ATPase activity

PMID:10713091

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0043531

ADP binding

PMID:11323718

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0051082

unfolded protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010236

F

Seeded from EcoCyc (v14.0)

complete

GO:0070417

cellular response to cold

PMID:7665466

IEP: Inferred from Expression Pattern

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rplJ

PMID:15690043

Experiment(s):EBI-878924, EBI-882104

Protein

secA

PMID:15690043

Experiment(s):EBI-878924

Protein

ygeQ

PMID:15690043

Experiment(s):EBI-882104

Protein

pepB

PMID:15690043

Experiment(s):EBI-882104

Protein

rplL

PMID:15690043

Experiment(s):EBI-882104

Protein

rplT

PMID:15690043

Experiment(s):EBI-882104

Protein

ugpB

PMID:15690043

Experiment(s):EBI-882104

Protein

rplQ

PMID:16606699

Experiment(s):EBI-1143130

Protein

rpsE

PMID:16606699

Experiment(s):EBI-1143130

Protein

ahpC

PMID:16606699

Experiment(s):EBI-1143130

Protein

rplE

PMID:16606699

Experiment(s):EBI-1143130

Protein

cheZ

PMID:16606699

Experiment(s):EBI-1143130

Protein

rpsC

PMID:16606699

Experiment(s):EBI-1143130

Protein

cedA

PMID:16606699

Experiment(s):EBI-1143130

Protein

chbA

PMID:16606699

Experiment(s):EBI-1143130

Protein

rpsI

PMID:16606699

Experiment(s):EBI-1143130

Protein

rpsD

PMID:16606699

Experiment(s):EBI-1143130

Protein

cybC

PMID:16606699

Experiment(s):EBI-1143130

Protein

ygjQ

PMID:16606699

Experiment(s):EBI-1143130

Protein

rplB

PMID:16606699

Experiment(s):EBI-1143130

Protein

rplF

PMID:16606699

Experiment(s):EBI-1143130

Protein

gnsA

PMID:16606699

Experiment(s):EBI-1143130

Protein

rplN

PMID:16606699

Experiment(s):EBI-1143130

Protein

fliT

PMID:16606699

Experiment(s):EBI-1143130

Protein

rplJ

PMID:19402753

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MALLQISEPG LSAAPHQRRL AAGIDLGTTN SLVATVRSGQ AETLADHEGR HLLPSVVHYQ
QQGHSVGYDA RTNAALDTAN TISSVKRLMG RSLADIQQRY PHLPYQFQAS ENGLPMIETA
AGLLNPVRVS ADILKALAAR ATEALAGELD GVVITVPAYF DDAQRQGTKD AARLAGLHVL
RLLNEPTAAA IAYGLDSGQE GVIAVYDLGG GTFDISILRL SRGVFEVLAT GGDSALGGDD
FDHLLADYIR EQAGIPDRSD NRVQRELLDA AIAAKIALSD ADSVTVNVAG WQGEISREQF
NELIAPLVKR TLLACRRALK DAGVEADEVL EVVMVGGSTR VPLVRERVGE FFGRPPLTSI
DPDKVVAIGA AIQADILVGN KPDSEMLLLD VIPLSLGLET MGGLVEKVIP RNTTIPVARA
QDFTTFKDGQ TAMSIHVMQG ERELVQDCRS LARFALRGIP ALPAGGAHIR VTFQVDADGL
LSVTAMEKST GVEASIQVKP SYGLTDSEIA SMIKDSMSYA EQDVKARMLA EQKVEAARVL
ESLHGALAAD AALLSAAERQ VIDDAAAHLS EVAQGDDVDA IEQAIKNVDK QTQDFAARRM
DQSVRRALKG HSVDEV
Length

616

Mol. Wt

65.652 kDa

pI

4.9 (calculated)

Extinction coefficient

20,400 - 20,650 (calc based on 10 Y, 1 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

21..601

PF00012 Hsp70 protein

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hscA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130451

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944885

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008315

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6Z1

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG12130

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12130

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944885

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002032

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2051

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

4.91E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

E. coli K-12 MG1655

2256

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

542

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

2252

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

hscBA-fdx

hscA-fdx

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2656937..2656977 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2526 (EcoliWiki Page)

NCBI GEO profiles for hscA

microarray

GenExpDB:b2526 (EcoliWiki Page)

Summary of data for hscA from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to hscA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12130

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2051

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2526

EcoGene

EcoGene:EG12130

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002032

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008315

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Xenopus tropicalis

  • ENSXETP00000019974 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Shigella flexneri

HSCA

From SHIGELLACYC

E. coli O157

HSCA

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00012 Hsp70 protein

Superfamily (EcoliWiki Page)

SUPERFAMILY:53067

Superfamily (EcoliWiki Page)

SUPERFAMILY:53067

Superfamily (EcoliWiki Page)

SUPERFAMILY:100920

Superfamily (EcoliWiki Page)

SUPERFAMILY:100934

EcoCyc

EcoCyc:EG12130

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12130

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002032

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2051

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008315

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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