hscA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

HscA

Synonyms

DnaK-like molecular chaperone specific for IscU[1], B2526[2][1], Hsc[2][1], HscA[2][1], Hsc66[2][1] , ECK2523, hsc, JW2510, b2526

Product description

chaperone, member of Hsp70 protein family[2][3]

Hsc66, DnaK-like chaperone, specific for IscU; involved in FtsZ-ring formation; HscB is the J-like co-chaperone for HscA[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005515

protein binding

PMID:11983079[5]

IPI: Inferred from Physical Interaction

UniProtKB:P0ACD4
UniProtKB:P0A6L9
UniProtKB:P0A9R9

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:12871959[6]

IPI: Inferred from Physical Interaction

UniProtKB:P0ACD4

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:15485839[7]

IPI: Inferred from Physical Interaction

UniProtKB:P0A6L9

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:19821612[8]

IPI: Inferred from Physical Interaction

EcoCyc:BIOTIN-SYN-CPLX

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001023

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010236

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

PMID:10713091[9]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

PMID:11323718[10]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0006457

protein folding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00679

P

Seeded from EcoCyc (v14.0)

complete

GO:0006457

protein folding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010236

P

Seeded from EcoCyc (v14.0)

complete

GO:0016226

iron-sulfur cluster assembly

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010236

P

Seeded from EcoCyc (v14.0)

complete

GO:0016226

iron-sulfur cluster assembly

PMID:10393315[11]

IGI: Inferred from Genetic Interaction

P

Seeded from EcoCyc (v14.0)

Missing: with/from

GO:0016226

iron-sulfur cluster assembly

PMID:10544286[12]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0016887

ATPase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00679

F

Seeded from EcoCyc (v14.0)

complete

GO:0016887

ATPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010236

F

Seeded from EcoCyc (v14.0)

complete

GO:0016887

ATPase activity

PMID:10713091[9]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0043531

ADP binding

PMID:11323718[10]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0051082

unfolded protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010236

F

Seeded from EcoCyc (v14.0)

complete

GO:0070417

cellular response to cold

PMID:7665466[13]

IEP: Inferred from Expression Pattern

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rplJ

PMID:15690043[14]

Experiment(s):EBI-878924, EBI-882104

Protein

secA

PMID:15690043[14]

Experiment(s):EBI-878924

Protein

ygeQ

PMID:15690043[14]

Experiment(s):EBI-882104

Protein

pepB

PMID:15690043[14]

Experiment(s):EBI-882104

Protein

rplL

PMID:15690043[14]

Experiment(s):EBI-882104

Protein

rplT

PMID:15690043[14]

Experiment(s):EBI-882104

Protein

ugpB

PMID:15690043[14]

Experiment(s):EBI-882104

Protein

rplQ

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

rpsE

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

ahpC

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

rplE

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

cheZ

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

rpsC

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

cedA

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

chbA

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

rpsI

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

rpsD

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

cybC

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

ygjQ

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

rplB

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

rplF

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

gnsA

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

rplN

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

fliT

PMID:16606699[15]

Experiment(s):EBI-1143130

Protein

rplJ

PMID:19402753[16]

LCMS(ID Probability):99.0 MALDI(Z-score):17.053160

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MALLQISEPG LSAAPHQRRL AAGIDLGTTN SLVATVRSGQ AETLADHEGR HLLPSVVHYQ
QQGHSVGYDA RTNAALDTAN TISSVKRLMG RSLADIQQRY PHLPYQFQAS ENGLPMIETA
AGLLNPVRVS ADILKALAAR ATEALAGELD GVVITVPAYF DDAQRQGTKD AARLAGLHVL
RLLNEPTAAA IAYGLDSGQE GVIAVYDLGG GTFDISILRL SRGVFEVLAT GGDSALGGDD
FDHLLADYIR EQAGIPDRSD NRVQRELLDA AIAAKIALSD ADSVTVNVAG WQGEISREQF
NELIAPLVKR TLLACRRALK DAGVEADEVL EVVMVGGSTR VPLVRERVGE FFGRPPLTSI
DPDKVVAIGA AIQADILVGN KPDSEMLLLD VIPLSLGLET MGGLVEKVIP RNTTIPVARA
QDFTTFKDGQ TAMSIHVMQG ERELVQDCRS LARFALRGIP ALPAGGAHIR VTFQVDADGL
LSVTAMEKST GVEASIQVKP SYGLTDSEIA SMIKDSMSYA EQDVKARMLA EQKVEAARVL
ESLHGALAAD AALLSAAERQ VIDDAAAHLS EVAQGDDVDA IEQAIKNVDK QTQDFAARRM
DQSVRRALKG HSVDEV
Length

616

Mol. Wt

65.652 kDa

pI

4.9 (calculated)

Extinction coefficient

20,400 - 20,650 (calc based on 10 Y, 1 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

21..601

PF00012 Hsp70 protein

PMID:19920124[17]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hscA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130451

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944885

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008315

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6Z1

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG12130

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12130

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944885

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002032

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2051

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Tokumoto, U et al. (2002) Network of protein-protein interactions among iron-sulfur cluster assembly proteins in Escherichia coli. J. Biochem. 131 713-9 PubMed
  6. ↑ Hoff, KG et al. (2003) Contributions of the LPPVK motif of the iron-sulfur template protein IscU to interactions with the Hsc66-Hsc20 chaperone system. J. Biol. Chem. 278 37582-9 PubMed
  7. ↑ Silberg, JJ et al. (2004) Regulation of the HscA ATPase reaction cycle by the co-chaperone HscB and the iron-sulfur cluster assembly protein IscU. J. Biol. Chem. 279 53924-31 PubMed
  8. ↑ Reyda, MR et al. (2009) A complex between biotin synthase and the iron-sulfur cluster assembly chaperone HscA that enhances in vivo cluster assembly. Biochemistry 48 10782-92 PubMed
  9. ↑ 9.0 9.1 Silberg, JJ & Vickery, LE (2000) Kinetic characterization of the ATPase cycle of the molecular chaperone Hsc66 from Escherichia coli. J. Biol. Chem. 275 7779-86 PubMed
  10. ↑ 10.0 10.1 Brehmer, D et al. (2001) Tuning of chaperone activity of Hsp70 proteins by modulation of nucleotide exchange. Nat. Struct. Biol. 8 427-32 PubMed
  11. ↑ Nakamura, M et al. (1999) Hyperproduction of recombinant ferredoxins in escherichia coli by coexpression of the ORF1-ORF2-iscS-iscU-iscA-hscB-hs cA-fdx-ORF3 gene cluster. J. Biochem. 126 10-8 PubMed
  12. ↑ Takahashi, Y & Nakamura, M (1999) Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli. J. Biochem. 126 917-26 PubMed
  13. ↑ Lelivelt, MJ & Kawula, TH (1995) Hsc66, an Hsp70 homolog in Escherichia coli, is induced by cold shock but not by heat shock. J. Bacteriol. 177 4900-7 PubMed
  14. ↑ 14.0 14.1 14.2 14.3 14.4 14.5 14.6 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  15. ↑ 15.00 15.01 15.02 15.03 15.04 15.05 15.06 15.07 15.08 15.09 15.10 15.11 15.12 15.13 15.14 15.15 15.16 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  16. ↑ Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  17. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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