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Standard Name	hisF
Gene Synonym(s)	ECK2020, b2025, JW2007^[1], JW2007
Product Desc.	imidazole glycerol phosphate synthase, HisF subunit^[2]^[3]; Component of imidazole glycerol phosphate synthase^[2]^[3] Cyclase component of imidazole glycerol phosphate (IGP) synthase^[4]
Product Synonyms(s)	imidazole glycerol phosphate synthase, catalytic subunit with HisH^[1], B2025^[2]^[1], HisF^[2]^[1], hisF enzyme^[2]^[1] , ECK2020, JW2007, b2025
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): hisBHAFI^[2], hisLGDCBHAFI^[2]
Regulation/Activity
Quick Links
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Notes

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N-terminus of purified HisF was sequenced by Klem and Davisson (1993), and the fMet is not clipped. Omitted from Verified set because natural RBS was not used. Natural RBS is very good and this is almost certainly the in vivo start.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hisF
Mnemonic	Histidine
Synonyms	ECK2020, b2025, JW2007^[1], JW2007
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	45.13 minutes, 45.13 minutes	MG1655: 2093868..2094644 <gbrowseImage> name=NC_000913:2093868..2094644 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2025846..2026622 <gbrowseImage> name=NC_012967:2025846..2026622 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1986351..1987127 <gbrowseImage> name=NC_012759:1986351..1987127 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2097981..2098757 <gbrowseImage> name=NC_007779:2097981..2098757 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2184876..2185652 <gbrowseImage> name=NC_010473:2184876..2185652 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔhisF (Keio:JW2007)	deletion	deletion	Auxotrophies	Requires histidine for growth	PMID:16738554	Shigen CGSC9653^[5]
hisFR5A		R5A	Auxotrophies	Loss of activity			seeded from UniProt:P60664
hisF::Tn5KAN-2 (FB20583)	Insertion at nt 391 in Plus orientation		Auxotrophies	Requires Histidine for growth	PMID:15262929	E. coli Genome Project:FB20583	contains pKD46
hisFR5H		R5H	Auxotrophies	Loss of IGP synthase activity. Weak IGP synthase activity and reduced hisH activity in vitro			seeded from UniProt:P60664
hisFE46G		E46G	Auxotrophies	Loss of IGP synthase activity. Weak IGP synthase and hisH activities in vitro			seeded from UniProt:P60664
hisFQ123A		Q123A	Auxotrophies	Decrease in activity			seeded from UniProt:P60664
hisFQ123R		Q123R	Auxotrophies	Loss of IGP synthase activity. Weak hisH activity in vitro			seeded from UniProt:P60664
hisFC124A		C124A	Auxotrophies	No change in activity			seeded from UniProt:P60664
hisFC124R		C124R	Auxotrophies	Loss of IGP synthase activity. Weak hisH activity in vitro			seeded from UniProt:P60664
hisFE46A		E46A	Auxotrophies	Loss of activity			seeded from UniProt:P60664
hisF860(Stable)			Auxotrophies	Requires Histidine for growth		CGSC:6983
hisF892			Auxotrophies	Requires Histidine for growth		CGSC:7338
ΔhisF723::kan	deletion	deletion	Auxotrophies	Requires Histidine for growth	PMID:16738554	CGSC:101368
hisF852			Energy metabolism	90% depletion of the ATP pool within 2 h of transfer to medium lacking histidine	PMID:2644255		Strain TA5090
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2007	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCTGGCAAAACGCATAATCCC Primer 2:CCACATATCCTGATCTCCACGCC
6D9	Kohara Phage	Genobase	PMID:3038334
21H10	Kohara Phage	Genobase	PMID:3038334
zef-3129::Tn10	Linked marker	CAG12099 = CGSC7397^[5]	est. P1 cotransduction: 69% ^[6] Synonyms:zee-3129::Tn10
zbd-3104::Tn10	Linked marker	CAG12021 = CGSC7344^[5]	est. P1 cotransduction: % ^[6] Synonyms:zbc-3105::Tn10, zbd-3105::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10448	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10448	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000441	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946516	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0443	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006727	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	HisF
Synonyms	imidazole glycerol phosphate synthase, catalytic subunit with HisH^[1], B2025^[2]^[1], HisF^[2]^[1], hisF enzyme^[2]^[1] , ECK2020, JW2007, b2025
Product description	imidazole glycerol phosphate synthase, HisF subunit^[2]^[3]; Component of imidazole glycerol phosphate synthase^[2]^[3] Cyclase component of imidazole glycerol phosphate (IGP) synthase^[4]
EC number (for enzymes)	2.4.2.-^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000105	histidine biosynthetic process	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01013	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000105	histidine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004651	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000105	histidine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006062	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000105	histidine biosynthetic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0368	P	Seeded from EcoCyc (v14.0)	complete
	GO:0000107	imidazoleglycerol-phosphate synthase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01013	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000107	imidazoleglycerol-phosphate synthase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004651	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016829	lyase activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0456	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of imidazole glycerol phosphate synthase	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MLAKRIIPCL DVRDGQVVKG VQFRNHEIIG DIVPLAKRYA EEGADELVFY DITASSDGRV VDKSWVSRVA EVIDIPFCVA GGIKSLEDAA KILSFGADKI SINSPALADP TLITRLADRF GVQCIVVGID TWYDAETGKY HVNQYTGDES RTRVTQWETL DWVQEVQKRG AGEIVLNMMN QDGVRNGYDL EQLKKVREVC HVPLIASGGA GTMEHFLEAF RDADVDGALA ASVFHKQIIN IGELKAYLAT QGVEIRIC
Length	258
Mol. Wt	28.453 kDa
pI	5.0 (calculated)
Extinction coefficient	32,430 - 33,055 (calc based on 7 Y, 4 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	5..240	PF00977 Histidine biosynthesis protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hisF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129966	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946516	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006727	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P60664	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10448	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10448	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946516	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000441	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0443	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MG1655	1002	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	989	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	494	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
hisBHAFI
hisLGDCBHAFI
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2093848..2093888 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2025 (EcoliWiki Page)	NCBI GEO profiles for hisF
microarray	GenExpDB:b2025 (EcoliWiki Page)	Summary of data for hisF from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to hisF Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10448	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0443	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2025
EcoGene	EcoGene:EG10448	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000441	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006727	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000001432 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YBR248C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC4181c (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	HISF	From SHIGELLACYC
E. coli O157	HISF	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00977 Histidine biosynthesis protein
Superfamily (EcoliWiki Page)	SUPERFAMILY:51366
Panther (EcoliWiki Page)	PTHR21235:SF2
EcoCyc	EcoCyc:EG10448	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10448	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000441	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0443	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0006727	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

hisF:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hisF Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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