hisF:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
hisF |
|---|---|
| Mnemonic |
Histidine |
| Synonyms |
ECK2020, b2025, JW2007[1], JW2007 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
45.13 minutes, 45.13 minutes |
MG1655: 2093868..2094644 |
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NC_012967: 2025846..2026622 |
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NC_012759: 1986351..1987127 |
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W3110 |
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W3110: 2097981..2098757 |
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DH10B: 2184876..2185652 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔhisF (Keio:JW2007) |
deletion |
deletion |
Auxotrophies |
Requires histidine for growth |
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hisFR5A |
R5A |
Auxotrophies |
Loss of activity |
seeded from UniProt:P60664 | |||
|
hisF::Tn5KAN-2 (FB20583) |
Insertion at nt 391 in Plus orientation |
Auxotrophies |
Requires Histidine for growth |
contains pKD46 | |||
|
hisFR5H |
R5H |
Auxotrophies |
Loss of IGP synthase activity. Weak IGP synthase activity and reduced hisH activity in vitro |
seeded from UniProt:P60664 | |||
|
hisFE46G |
E46G |
Auxotrophies |
Loss of IGP synthase activity. Weak IGP synthase and hisH activities in vitro |
seeded from UniProt:P60664 | |||
|
hisFQ123A |
Q123A |
Auxotrophies |
Decrease in activity |
seeded from UniProt:P60664 | |||
|
hisFQ123R |
Q123R |
Auxotrophies |
Loss of IGP synthase activity. Weak hisH activity in vitro |
seeded from UniProt:P60664 | |||
|
hisFC124A |
C124A |
Auxotrophies |
No change in activity |
seeded from UniProt:P60664 | |||
|
hisFC124R |
C124R |
Auxotrophies |
Loss of IGP synthase activity. Weak hisH activity in vitro |
seeded from UniProt:P60664 | |||
|
hisFE46A |
E46A |
Auxotrophies |
Loss of activity |
seeded from UniProt:P60664 | |||
|
hisF860(Stable) |
Auxotrophies |
Requires Histidine for growth |
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hisF892 |
Auxotrophies |
Requires Histidine for growth |
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ΔhisF723::kan |
deletion |
deletion |
Auxotrophies |
Requires Histidine for growth |
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|
hisF852 |
Energy metabolism |
90% depletion of the ATP pool within 2 h of transfer to medium lacking histidine |
Strain TA5090 | ||||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2007 |
Plasmid clone |
Status:Clone OK Primer 1:GCCCTGGCAAAACGCATAATCCC Primer 2:CCACATATCCTGATCTCCACGCC | |
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Kohara Phage |
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Kohara Phage |
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zef-3129::Tn10 |
Linked marker |
est. P1 cotransduction: 69% [8] | |
|
Linked marker |
est. P1 cotransduction: % [8] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Joshi, AK et al. (1989) Energy coupling in bacterial periplasmic transport systems. Studies in intact Escherichia coli cells. J. Biol. Chem. 264 2126-33 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
