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Standard Name	hipA
Gene Synonym(s)	ECK1500, b1507, JW1500^[1], JW1500
Product Desc.	HipA Polypeptide^[2]; serine kinase HipA^[3]; Component of HipB transcriptional repressor^[3] Toxin, growth inhibition blocked by continuous HipB synthesis;; probable role in cell division^[4]
Product Synonyms(s)	regulator with hipB^[1], B1507^[2]^[1], HipA^[2]^[1] , ECK1500, JW1500, b1507
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): hipBA^[2]
Regulation/Activity
Quick Links
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Notes

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HipA serine protein kinase autophosphorylates Ser150 and this activity is essential for persister formation (Correia, 2006). HipA binds and phosphorylates TufA/TufB (Schumacher, 2009).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hipA
Mnemonic	High persistence
Synonyms	ECK1500, b1507, JW1500^[1], JW1500
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	34.25 minutes	MG1655: 1590200..1588878 <gbrowseImage> name=NC_000913:1588878..1590200 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1568743..1567421 <gbrowseImage> name=NC_012967:1567421..1568743 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1480937..1482259 <gbrowseImage> name=NC_012759:1480937..1482259 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1593890..1592568 <gbrowseImage> name=NC_007779:1592568..1593890 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1679825..1678503 <gbrowseImage> name=NC_010473:1678503..1679825 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
ΔhipA (Keio:JW1500)	deletion	deletion			PMID:16738554	Shigen CGSC9299^[5]
hipA::Tn5KAN-2 (FB20340)	Insertion at nt 180 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20340	contains pKD46
hipA::Tn5KAN-2 (FB20341)	Insertion at nt 180 in Plus orientation				PMID:15262929	E. coli Genome Project:FB20341	does not contain pKD46
hipA7		G22S and D291A	Growth Phenotype	The hipA7 allele confers a high persistence phenotype and cold sensitivity at 20°C. The HipA7 protein is non-toxic.	PMID:14622409		Persisters are cells in a population that survive antibiotic treatment even though genetically they remain sensitive to the antibiotic. Cultures of wild-type cells typically contain 10-5 to 10-6 persister cells. Cultures of hipA7 cells contain 10-2 to 10-3 persister cells.
ΔhipA728::kan					PMID:16738554	CGSC:101358
hipA7		G22S and D291A	presistence frequency	The hipA7 allele confers a high persistence phenotype.	PMID:6348026	experimental strain:HM22	Persisters are cells in a population that survive antibiotic treatment even though genetically they remain sensitive to the antibiotic. Cultures of wild-type cells typically contain 10-5 to 10-6 persister cells. Cultures of hipA7 cells contain 10-2 to 10-3 persister cells.
hipA9		G22S and D291A	presistence frequency	The hipA9 allele confers a high persistence phenotype.	PMID:6348026	experimental strain:HM23	Persisters are cells in a population that survive antibiotic treatment even though genetically they remain sensitive to the antibiotic. Cultures of wild-type cells typically contain 10-5 to 10-6 persister cells. Cultures of hipA9 cells contain 10-2 to 10-3 persister cells.

hipA9		G22S and D291A	presistence frequency	The hipA9 allele confers a high persistence phenotype.	PMID:196328	experimental strain:HM23	Persisters are cells in a population that survive antibiotic treatment even though genetically they remain sensitive to the antibiotic. Cultures of wild-type cells typically contain 10-5 to 10-6 persister cells. Cultures of hipA7 cells contain 10-2 to 10-3 persister cells. Refer to figure 3.
hipA7		G22S and D291A	presistence frequency	The hipA7 allele confers a high persistence phenotype.	PMID:1963289	experimental strain:HM22	Persisters are cells in a population that survive antibiotic treatment even though genetically they remain sensitive to the antibiotic. Cultures of wild-type cells typically contain 10-5 to 10-6 persister cells. Cultures of hipA7 cells contain 10-2 to 10-3 persister cells.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1500	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCCTAAACTTGTCACTTGGAT Primer 2:CCtTTACTACCGTATTCTCGGCT
6E8	Kohara Phage	Genobase	PMID:3038334
2A9	Kohara Phage	Genobase	PMID:3038334
5F9	Kohara Phage	Genobase	PMID:3038334
zdd-235::Tn10	Linked marker	CAG18461 = CGSC7378^[5]	est. P1 cotransduction: 8% ^[6] Synonyms:zdc-235::Tn10
zdi-925::Tn10	Linked marker	CAG12151 = CGSC7385^[5]	est. P1 cotransduction: % ^[6] Synonyms:zdh-925::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10443	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10443	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000436	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946064	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0438	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005022	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	HipA
Synonyms	regulator with hipB^[1], B1507^[2]^[1], HipA^[2]^[1] , ECK1500, JW1500, b1507
Product description	HipA Polypeptide^[2]; serine kinase HipA^[3]; Component of HipB transcriptional repressor^[3] Toxin, growth inhibition blocked by continuous HipB synthesis;; probable role in cell division^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of HipB transcriptional repressor	could be indirect
Protein	tehB		PMID:19402753	MALDI(Z-score):29.262247
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MPKLVTWMNN QRVGELTKLA NGAHTFKYAP EWLASRYARP LSLSLPLQRG NITSDAVFNF FDNLLPDSPI VRDRIVKRYH AKSRQPFDLL SEIGRDSVGA VTLIPEDETV THPIMAWEKL TEARLEEVLT AYKADIPLGM IREENDFRIS VAGAQEKTAL LRIGNDWCIP KGITPTTHII KLPIGEIRQP NATLDLSQSV DNEYYCLLLA KELGLNVPDA EIIKAGNVRA LAVERFDRRW NAERTVLLRL PQEDMCQTFG LPSSVKYESD GGPGIARIMA FLMGSSEALK DRYDFMKFQV FQWLIGATDG HAKNFSVFIQ AGGSYRLTPF YDIISAFPVL GGTGIHISDL KLAMGLNASK GKKTAIDKIY PRHFLATAKV LRFPEVQMHE ILSDFARMIP AALDNVKTSL PTDFPENVVT AVESNVLRLH GRLSREYGSK
Length	440
Mol. Wt	49.277 kDa
pI	8.3 (calculated)
Extinction coefficient	50,880 - 51,255 (calc based on 12 Y, 6 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Modification Site	150	phosphorylation site at S150	probability greater than 75%	PMID:17938405
Domain	249..332	PF07804 HipA-like C-terminal domain		PMID:19920124
Domain	150..238	PF07805 HipA-like N-terminal domain		PMID:19920124
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<motif_map/>

Structure
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Structures

<beststructure> gene=hipA taxon=562,83333 </beststructure>

Models

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129466	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946064	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005022	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P23874	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10443	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10443	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946064	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000436	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0438	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.17E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	4.308+/-0.162	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.018910741	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	134	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	62	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	101	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
hipBA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1590180..1590220 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1507 (EcoliWiki Page)	NCBI GEO profiles for hipA
microarray	GenExpDB:b1507 (EcoliWiki Page)	Summary of data for hipA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to hipA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10443	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0438	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1507
EcoGene	EcoGene:EG10443	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000436	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005022	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000036642 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
E. coli O157	HIPA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF07804 HipA-like C-terminal domain
Pfam (EcoliWiki Page)	PF07805 HipA-like N-terminal domain
EcoCyc	EcoCyc:EG10443	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10443	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000436	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0438	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005022	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

hipA:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hipA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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