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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	hflX
Gene Synonym(s)	ECK4169, b4173, JW4131, hflA, hslX^[1], hslX
Product Desc.	putative GTPase; possible regulator of HflKC^[2]^[3] GTPase, stimulated by 50S subunit binding, function unknown^[4]
Product Synonyms(s)	predicted GTPase^[1], B4173^[2]^[1], HflA^[2]^[1], HslX^[2]^[1], HflX^[2]^[1] , ECK4169, hflA, hslX, JW4131, b4173
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yjeFE-amiB-mutL-miaA-hfq-hflXKC^[2], ORF55-ORF17-amiB-mutL-miaA-hfq-hflXKC
Regulation/Activity
Quick Links
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Notes

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HflX has both GTPase and ATPase activities (Jain, 2009; Dutta, 2009). HflX itself does not affect transposition efficiency and an earlier report of a defect may be due to a polar effect on hflKC (Twiss, 2005; Dutta, 2009).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hflX
Mnemonic	High frequency lysogenization
Synonyms	ECK4169, b4173, JW4131, hflA, hslX^[1], hslX
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	94.81 minutes	MG1655: 4398695..4399975 <gbrowseImage> name=NC_000913:4398695..4399975 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4378827..4380107 <gbrowseImage> name=NC_012967:4378827..4380107 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4337430..4338710 <gbrowseImage> name=NC_012759:4337430..4338710 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4405352..4406632 <gbrowseImage> name=NC_007779:4405352..4406632 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4499057..4500337 <gbrowseImage> name=NC_010473:4499057..4500337 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔhflX (Keio:JW4131)	deletion	deletion	PMID:16738554	Shigen CGSC10974^[5]
ΔhflX723::kan			PMID:16738554	CGSC:101352
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4131	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTTTGACCGTTATGATGCTGG Primer 2:CCGATCAGGTAATCGATCAACGC
3A1	Kohara Phage	Genobase	PMID:3038334
cadB2231::Tn10	Linked marker	CAG18488 = CGSC7479^[5]	est. P1 cotransduction: 16% ^[6] Synonyms:zjd-2231::Tn10
cycA30::Tn10	Linked marker	CAG12073 = CGSC7482^[5]	est. P1 cotransduction: 35% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10437	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10437	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000430	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948688	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0432	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013661	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	HflX
Synonyms	predicted GTPase^[1], B4173^[2]^[1], HflA^[2]^[1], HslX^[2]^[1], HflX^[2]^[1] , ECK4169, hflA, hslX, JW4131, b4173
Product description	putative GTPase; possible regulator of HflKC^[2]^[3] GTPase, stimulated by 50S subunit binding, function unknown^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005525	GTP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002917	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005525	GTP binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006073	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009408	response to heat	PMID:8349564	IEP: Inferred from Expression Pattern		P		complete
	GO:0005525	GTP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0342	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005737	cytoplasm	PMID:19181811	IDA: Inferred from Direct Assay		C		complete
	GO:0005622	intracellular	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002917	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005525	GTP binding	PMID:19181811	IDA: Inferred from Direct Assay		F		complete
	GO:0005524	ATP binding	PMID:19181811	IDA: Inferred from Direct Assay		F		complete
NOT	GO:0030069	lysogeny	PMID:19181811	IMP: Inferred from Mutant Phenotype		P		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MFDRYDAGEQ AVLVHIYFTQ DKDMEDLQEF ESLVSSAGVE ALQVITGSRK APHPKYFVGE GKAVEIAEAV KATGASVVLF DHALSPAQER NLERLCECRV IDRTGLILDI FAQRARTHEG KLQVELAQLR HLATRLVRGW THLERQKGGI GLRGPGETQL ETDRRLLRNR IVQIQSRLER VEKQREQGRQ SRIKADVPTV SLVGYTNAGK STLFNRITEA RVYAADQLFA TLDPTLRRID VADVGETVLA DTVGFIRHLP HDLVAAFKAT LQETRQATLL LHVIDAADVR VQENIEAVNT VLEEIDAHEI PTLLVMNKID MLEDFEPRID RDEENKPNRV WLSAQTGAGI PQLFQALTER LSGEVAQHTL RLPPQEGRLR SRFYQLQAIE KEWMEEDGSV SLQVRMPIVD WRRLCKQEPA LIDYLI
Length	426
Mol. Wt	48.326 kDa
pI	5.8 (calculated)
Extinction coefficient	32,430 - 32,805 (calc based on 7 Y, 4 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	209..318	PF01926 GTPase of unknown function		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hflX taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131995	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948688	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013661	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P25519	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10437	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10437	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948688	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000430	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0432	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	1.67E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	684	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	374	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	591	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yjeFE-amiB-mutL-miaA-hfq-hflXKC
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4398675..4398715 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4173 (EcoliWiki Page)	NCBI GEO profiles for hflX
microarray	GenExpDB:b4173 (EcoliWiki Page)	Summary of data for hflX from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to hflX Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10437	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0432	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4173
EcoGene	EcoGene:EG10437	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000430	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013661	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000014224 (score: 1.000; bootstrap: 97%)	From Inparanoid:20070104
Arabidopsis thaliana	AT5G57960 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000018122 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00038607 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00009769 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000016099 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0039339 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA18671-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000026928 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Homo sapiens	ENSP00000316598 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000018260 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000000388 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Mus musculus	MGI:1306825 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q6ATJ0 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000033160 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000165617 (score: 1.000; bootstrap: 99%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00020148001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000008922 (score: 1.000; bootstrap: 98%)	From Inparanoid:20070104
Shigella flexneri	HFLX	From SHIGELLACYC
E. coli O157	HFLX	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF01926 GTPase of unknown function
Superfamily (EcoliWiki Page)	SUPERFAMILY:52540
Panther (EcoliWiki Page)	PTHR10229:SF0
Superfamily (EcoliWiki Page)	SUPERFAMILY:54980
EcoCyc	EcoCyc:EG10437	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10437	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000430	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0432	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0013661	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

hflX:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hflX Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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