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Expression|

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Standard Name	hcaT
Gene Synonym(s)	ECK2533, b2536, JW2520, yfhS^[1], yfhS
Product Desc.	HcaT MFS transporter^[2]^[3] Probable 3-phenylpropionic acid/cinnamic acid transporter; hca genes catabolize 3-phenylpropionate and cinnamic acid, feeding the products into the the mhp pathway^[4]
Product Synonyms(s)	predicted 3-phenylpropionic transporter^[1], B2536^[2]^[1], HcaT^[2]^[1], YfhS^[2]^[1] , ECK2533, JW2520, yfhS, b2536
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): hcaT^[2]
Regulation/Activity
Quick Links
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Notes

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Major Facilitator Superfamily (MFS). Hydrocinnamic acid is a synonym for 3-phenylpropionic acid.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	hcaT
Mnemonic	Hydrocinnamic acid
Synonyms	ECK2533, b2536, JW2520, yfhS^[1], yfhS
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	57.43 minutes	MG1655: 2665868..2664729 <gbrowseImage> name=NC_000913:2664729..2665868 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2588545..2587406 <gbrowseImage> name=NC_012967:2587406..2588545 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2550534..2551673 <gbrowseImage> name=NC_012759:2550534..2551673 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 2666502..2665363 <gbrowseImage> name=NC_007779:2665363..2666502 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 2757633..2756494 <gbrowseImage> name=NC_010473:2756494..2757633 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔhcaT (Keio:JW2520)	deletion	deletion	PMID:16738554	Shigen CGSC10006^[5]
zfh-208::Tn10	Insertion at 2,665,161 bp in MG1655 (NC_000913)		adapted from Nichols et al.^[6]	CAG18481 = CGSC7417^[5]	Synonyms: zff-208::Tn10
ΔhcaT781::kan			PMID:16738554	CGSC:101318
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2520	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGTTTTGCAATCCACGCGCTG Primer 2:CCGCATGAGGGAACAACTTTCGG
5E10	Kohara Phage	Genobase	PMID:3038334
6F10	Kohara Phage	Genobase	PMID:3038334
purC80::Tn10	Linked marker	CAG18470 = CGSC7413^[5]	est. P1 cotransduction: 1% ^[6]
nadB51::Tn10	Linked marker	CAG18480 = CGSC7419^[5]	est. P1 cotransduction: 14% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7330	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13454	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003902	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947007	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3227	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008348	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	HcaT
Synonyms	predicted 3-phenylpropionic transporter^[1], B2536^[2]^[1], HcaT^[2]^[1], YfhS^[2]^[1] , ECK2533, JW2520, yfhS, b2536
Product description	HcaT MFS transporter^[2]^[3] Probable 3-phenylpropionic acid/cinnamic acid transporter; hca genes catabolize 3-phenylpropionate and cinnamic acid, feeding the products into the the mhp pathway^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000576	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015293	symporter activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0769	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000576	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
plasma membrane
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MVLQSTRWLA LGYFTYFFSY GIFLPFWSVW LKGIGLTPET IGLLLGAGLV ARFLGSLLIA PRVSDPSRLI SALRVLALLT LLFAVAFWAG AHVAWLMLVM IGFNLFFSPL VPLTDALANT WQKQFPLDYG KVRLWGSVAF VIGSALTGKL VTMFDYRVIL ALLTLGVASM LLGFLIRPTI QPQGASRQQE STGWSAWLAL VRQNWRFLAC VCLLQGAHAA YYGFSAIYWQ AAGYSASAVG YLWSLGVVAE VIIFALSNKL FRRCSARDML LISAICGVVR WGIMGATTAL PWLIVVQILH CGTFTVCHLA AMRYIAARQG SEVIRLQAVY SAVAMGGSIA IMTVFAGFLY QYLGHGVFWV MALVALPAMF LRPKVVPSC
Length	379
Mol. Wt	41.593 kDa
pI	10.3 (calculated)
Extinction coefficient	103,360 - 104,235 (calc based on 14 Y, 15 W, and 7 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hcaT taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130461	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947007	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008348	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:Q47142	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G7330	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13454	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947007	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003902	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3227	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	116	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	20	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	46^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
hcaT
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:2665848..2665888 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2536 (EcoliWiki Page)	NCBI GEO profiles for hcaT
microarray	GenExpDB:b2536 (EcoliWiki Page)	Summary of data for hcaT from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (2665776..2666083) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ16; Well:F5^[7]
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Notes

Accessions Related to hcaT Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G7330	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3227	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2536
EcoGene	EcoGene:EG13454	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003902	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008348	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000016938 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00030798 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000006447 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000141117 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	HCAT	From SHIGELLACYC
E. coli O157	HCAT	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Panther (EcoliWiki Page)	PTHR24003:SF387
Superfamily (EcoliWiki Page)	SUPERFAMILY:103473
EcoCyc	EcoCyc:G7330	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13454	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003902	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3227	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0008348	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 ^6.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

hcaT:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to hcaT Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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