greA:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
greA |
|---|---|
| Mnemonic |
Growth regulator |
| Synonyms |
ECK3170, b3181, JW3148[1], JW3148 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
71.69 minutes |
MG1655: 3326737..3326261 |
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NC_012967: 3263949..3263473 |
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NC_012759: 3213409..3213885 |
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W3110 |
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W3110: 3328570..3328094 |
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DH10B: 3424482..3424006 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
3326261 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
greA(del) (Keio:JW3148) |
deletion |
deletion |
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greA::Tn5KAN-I-SceI (FB21062) |
Insertion at nt 212 in Plus orientation |
contains pKD46 | |||||
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greA788(del)::kan |
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greA D41N |
A single Amino Acid was substituted. |
D41N |
Gene Expression |
Reduced lambda red-mediated recombination |
See Table 1 | ||
|
greA D41A |
A single Amino Acid was changed. |
D41A |
Gene Expression |
Reduced lambda red-mediated recombination |
See Table 1 | ||
|
greA E44K |
A single amino acid is substituted |
E44K |
Gene Expression |
Reduced lambda red-mediated recombination |
See table 1. | ||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3148 |
Plasmid clone |
Status:Clone OK Primer 1:GCCCAAGCTATTCCGATGACCTT Primer 2:CCaAGGTATTCCACCTTAATTAC | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: % [9] | ||
|
Linked marker |
est. P1 cotransduction: 85% [9] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Borukhov, S et al. (1992) GreA protein: a transcription elongation factor from Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 89 8899-902 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ 6.0 6.1 6.2 Poteete, AR (2011) Recombination phenotypes of Escherichia coli greA mutants. BMC Mol. Biol. 12 12 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
