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Gene |

Product(s) |

Expression|

Evolution |

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Standard Name	gntT
Gene Synonym(s)	ECK3402, b3415, JW5690, gntM, usgA, gntS, gntTalpha, yhgC, G419^[1]^[2]
Product Desc.	GntT Gluconate Gnt transporter^[2]^[3] High-affinity gluconate transport^[4]
Product Synonyms(s)	gluconate transporter, high-affinity GNT I system^[1], B3415^[2]^[1], YhgC^[2]^[1], UsgA^[2]^[1], GntS^[2]^[1], GntM^[2]^[1], GntT^[2]^[1] , ECK3402, gntM, gntTalpha, JW5690, usgA, yhgC, b3415
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): gntT^[2], OP00324
Regulation/Activity
Quick Links
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Notes

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IT (Ion Transporter) superfamily (Prakash, 2003). ST(3) structural class based on hydropathy profile (Lolkema, 2003).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	gntT
Mnemonic	Gluconate
Synonyms	ECK3402, b3415, JW5690, gntM, usgA, gntS, gntTalpha, yhgC, G419^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	76.4 minutes	MG1655: 3544581..3545897 <gbrowseImage> name=NC_000913:3544581..3545897 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3475225..3476541 <gbrowseImage> name=NC_012967:3475225..3476541 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3433063..3434391 <gbrowseImage> name=NC_012759:3433063..3434391 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4093857..4092541 <gbrowseImage> name=NC_007779:4092541..4093857 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3642326..3643642 <gbrowseImage> name=NC_010473:3642326..3643642 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
ΔgntT (Keio:JW5690)	deletion	deletion	PMID:16738554	Shigen
gntT::Tn5KAN-I-SceI (FB21208)	Tn5 insertion in minus orientation at position 951.		PMID:12519969	ASAP ASAP strain name: FB21183	Note: FB21183 contains plasmid pKD46.
gntT::Tn5KAN-I-SceI (FB21208)	Tn5 insertion in minus orientation at position 951.		PMID:12519969	ASAP ASAP strain name: FB21184	Note: Strain FB21184 doesn't contain plasmid pKD46.
gntT1				CGSC:7907
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW5690	Plasmid clone	Shigen	PMID:16769691 Status:cloned as old JW Primer 1:GCCCCATTAGTCATTGTTGCTAT Primer 2:CCAATCACCATATTCAGCAGCAG
E4E4	Kohara Phage	Genobase	PMID:3038334
2F6	Kohara Phage	Genobase	PMID:3038334
zhf-3084::Tn10	Linked marker	CAG18456 = CGSC7576^[5]	est. P1 cotransduction: 17% ^[6] Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).
glgP721::Tn10	Linked marker	CAG18638 = CGSC7451^[5]	est. P1 cotransduction: 51% ^[6] Synonyms:zhg-3086::Tn10, zhh-21::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12380	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12380	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002247	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947924	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2282	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011148	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	GntT
Synonyms	gluconate transporter, high-affinity GNT I system^[1], B3415^[2]^[1], YhgC^[2]^[1], UsgA^[2]^[1], GntS^[2]^[1], GntM^[2]^[1], GntT^[2]^[1] , ECK3402, gntM, gntTalpha, JW5690, usgA, yhgC, b3415
Product description	GntT Gluconate Gnt transporter^[2]^[3] High-affinity gluconate transport^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0008643	carbohydrate transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0762	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015128	gluconate transmembrane transporter activity	PMID:9358057	IMP: Inferred from Mutant Phenotype		F	Assayed gluconate transport in a mutant strain transformed with either pGNTT20, which carries the gntT gene, or pBR322 (Fig. 2).	complete
	GO:0035429	gluconate transmembrane transport	PMID:9358057	IMP: Inferred from Mutant Phenotype		P	Fig. 2	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003474	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0019521	D-gluconate metabolic process	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0311	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005887	integral to plasma membrane	PMID:9045817	ISM: Inferred from Sequence Model		C	TOPPRED^[7]	Missing: with/from
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source
plasma membrane	C-terminus localized to the periplasm with 10 predicted transmembrane domains	Daley et al. (2005) ^[8]
plasma membrane			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MPLVIVAIGV ILLLLLMIRF KMNGFIALVL VALAVGLMQG MPLDKVIGSI KAGVGGTLGS LALIMGFGAM LGKMLADCGG AQRIATTLIA KFGKKHIQWA VVLTGFTVGF ALFYEVGFVL MLPLVFTIAA SANIPLLYVG VPMAAALSVT HGFLPPHPGP TAIATIFNAD MGKTLLYGTI LAIPTVILAG PVYARVLKGI DKPIPEGLYS AKTFSEEEMP SFGVSVWTSL VPVVLMAMRA IAEMILPKGH AFLPVAEFLG DPVMATLIAV LIAMFTFGLN RGRSMDQIND TLVSSIKIIA MMLLIIGGGG AFKQVLVDSG VDKYIASMMH ETNISPLLMA WSIAAVLRIA LGSATVAAIT AGGIAAPLIA TTGVSPELMV IAVGSGSVIF SHVNDPGFWL FKEYFNLTIG ETIKSWSMLE TIISVCGLVG CLLLNMVI
Length	438
Mol. Wt	45.968 kDa
pI	8.4 (calculated)
Extinction coefficient	37,930 - 38,305 (calc based on 7 Y, 5 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	1..437	PF02447 GntP family permease		PMID:19920124
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gntT taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:49176350	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947924	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011148	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P39835	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12380	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12380	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947924	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002247	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2282	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.67E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	E. coli K-12 MG1655	66	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	58	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	11^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
gntT
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3544561..3544601 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3415 (EcoliWiki Page)	NCBI GEO profiles for gntT
microarray	GenExpDB:b3415 (EcoliWiki Page)	Summary of data for gntT from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (3543758..3544269) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ16; Well:H9^[9]
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Notes

Accessions Related to gntT Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12380	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2282	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3415
EcoGene	EcoGene:EG12380	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002247	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011148	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	GNTT	From SHIGELLACYC
E. coli O157	GNTT	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02447 GntP family permease
EcoCyc	EcoCyc:EG12380	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12380	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002247	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2282	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011148	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 ^1.14 ^1.15 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 ^2.16 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ von Heijne, G (1992) Membrane protein structure prediction. Hydrophobicity analysis and the positive-inside rule. J. Mol. Biol. 225 487-94 PubMed
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

gntT:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to gntT Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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