PMID:9358057
| Citation |
Izu, H, Kawai, T, Yamada, Y, Aoshima, H, Adachi, O and Yamada, M (1997) Characterization of the gntT gene encoding a high-affinity gluconate permease in Escherichia coli. Gene 199:203-10 |
|---|---|
| Abstract |
We characterized the gntT gene encoding a high-affinity gluconate permease of Escherichia coli K-12. Primer extension and lacZ-operon fusion analyses revealed that gntT has one strong and two weak promoters, all of which are regulated positively by cAMP-CRP and negatively by GntR. The weak promoters became constitutive when separated from the upstream region including the strong promoter that overlaps a putative GntR-binding sequence. Gluconate-specific uptake activity was observed with cells harboring the gntT plasmid clone, which was enhanced by the presence of gntK encoding gluconate kinase. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Biological Transport; Carbohydrates; Carrier Proteins; Cyclic AMP/pharmacology; Cyclic AMP Receptor Protein/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial/drug effects; Gene Expression Regulation, Bacterial/physiology; Genes, Bacterial/physiology; Gluconates/metabolism; Gluconates/pharmacology; Kinetics; Membrane Transport Proteins/genetics; Molecular Sequence Data; Operator Regions, Genetic/genetics; Phosphotransferases (Alcohol Group Acceptor)/physiology; Promoter Regions, Genetic/genetics; Sequence Analysis, DNA |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
