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Product(s) |

Expression|

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Standard Name	gloA
Gene Synonym(s)	ECK1647, b1651, JW1643^[1], JW1643
Product Desc.	GloA^[2]^[3]; Component of glyoxalase I^[2]^[3] Glyoxalase I, lactoylglutathione lyase, Ni(2+) cofactor; inactivated by zinc; dimeric^[4]
Product Synonyms(s)	glyoxalase I, Ni-dependent^[1], B1651^[2]^[1], GloA^[2]^[1] , ECK1647, JW1643, b1651
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): gloA^[2]
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	gloA
Mnemonic	Glyoxalase
Synonyms	ECK1647, b1651, JW1643^[1], JW1643
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	37.2 minutes	MG1655: 1725861..1726268 <gbrowseImage> name=NC_000913:1725861..1726268 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1705031..1705438 <gbrowseImage> name=NC_012967:1705031..1705438 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1617920..1618327 <gbrowseImage> name=NC_012759:1617920..1618327 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1729551..1729958 <gbrowseImage> name=NC_007779:1729551..1729958 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1816432..1816839 <gbrowseImage> name=NC_010473:1816432..1816839 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	1725861	Edman degradation	PMID:9628737
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔgloA (Keio:JW1643)	deletion	deletion	PMID:16738554	Shigen CGSC9398^[5]
ΔgloA729::kan			PMID:16738554	CGSC:101172
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1643	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCGTCTTCTTCATACCATGCT Primer 2:CCGTTGCCCAGACCGCGACCGGC
6F11	Kohara Phage	Genobase	PMID:3038334
4H6	Kohara Phage	Genobase	PMID:3038334
zdd-235::Tn10	Linked marker	CAG18461 = CGSC7378^[5]	est. P1 cotransduction: % ^[6] Synonyms:zdc-235::Tn10
zdi-925::Tn10	Linked marker	CAG12151 = CGSC7385^[5]	est. P1 cotransduction: 9% ^[6] Synonyms:zdh-925::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6891	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13421	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003480	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946161	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3197	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005520	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	GloA
Synonyms	glyoxalase I, Ni-dependent^[1], B1651^[2]^[1], GloA^[2]^[1] , ECK1647, JW1643, b1651
Product description	GloA^[2]^[3]; Component of glyoxalase I^[2]^[3] Glyoxalase I, lactoylglutathione lyase, Ni(2+) cofactor; inactivated by zinc; dimeric^[4]
EC number (for enzymes)	4.4.1.5^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0004462	lactoylglutathione lyase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004361	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004462	lactoylglutathione lyase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR018146	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004462	lactoylglutathione lyase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:4.4.1.5	F	Seeded from EcoCyc (v14.0)	complete
	GO:0016151	nickel ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0533	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of glyoxalase I	could be indirect
Protein	dnaK		PMID:15690043	Experiment(s):EBI-884394, EBI-891329
Protein	creC		PMID:15690043	Experiment(s):EBI-891329
Protein	groL		PMID:15690043	Experiment(s):EBI-891329
Protein	pta		PMID:19402753	LCMS(ID Probability):98.9
Protein	groL		PMID:19402753	LCMS(ID Probability):99.6
Protein	groS		PMID:19402753	LCMS(ID Probability):99.6
Protein	slyD		PMID:19402753	LCMS(ID Probability):99.6
Protein	dnaE		PMID:19402753	MALDI(Z-score):18.801583
Protein	dnaX		PMID:19402753	MALDI(Z-score):23.842581
Protein	creC		PMID:19402753	LCMS(ID Probability):99.6
Protein	speA		PMID:19402753	MALDI(Z-score):21.143305
Protein	nikA		PMID:19402753	MALDI(Z-score):25.024707
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MRLLHTMLRV GDLQRSIDFY TKVLGMKLLR TSENPEYKYS LAFVGYGPET EEAVIELTYN WGVDKYELGT AYGHIALSVD NAAEACEKIR QNGGNVTREA GPVKGGTTVI AFVEDPDGYK IELIEEKDAG RGLGN
Length	135
Mol. Wt	14.919 kDa
pI	4.8 (calculated)
Extinction coefficient	17,420 - 17,545 (calc based on 8 Y, 1 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	2..123	PF00903 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gloA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129609	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946161	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005520	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AC81	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G6891	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13421	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946161	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003480	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3197	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	5.18E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	98.724+/-0.441	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.138820639	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	5210	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	2525	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	2597	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
gloA
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1725841..1725881 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1651 (EcoliWiki Page)	NCBI GEO profiles for gloA
microarray	GenExpDB:b1651 (EcoliWiki Page)	Summary of data for gloA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1725709..1725929) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ06; Well:G1^[7]
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Notes

Accessions Related to gloA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G6891	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3197	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1651
EcoGene	EcoGene:EG13421	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003480	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005520	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000016950 (score: 1.000; bootstrap: 53%) ENSANGP00000009226 (score: 1.000; bootstrap: 55%)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000033644 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT1G67280 (score: 1.000; bootstrap: 99%) AT1G11840 (score: 0.490)	From Inparanoid:20070104
Bos taurus	ENSBTAP00000010283 (score: 1.000; bootstrap: 81%) ENSBTAP00000016884 (score: 0.677)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00039226 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00006448 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000002151 (score: 1.000; bootstrap: 79%)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000012805 (score: 1.000; bootstrap: 67%)	From Inparanoid:20070104
Danio rerio	ZDB-GENE-030722-9 (score: 1.000; bootstrap: 72%) ZDB-CDNA-040425-3115 (score: 1.000; bootstrap: 72%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0230987 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0033162 (score: 1.000; bootstrap: 61%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000016450 (score: 1.000; bootstrap: 80%)	From Inparanoid:20070104
Homo sapiens	ENSP00000244746 (score: 1.000; bootstrap: 77%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000002187 (score: 1.000; bootstrap: 77%)	From Inparanoid:20070104
Monodelphis domestica	ENSMODP00000016559 (score: 1.000; bootstrap: 74%)	From Inparanoid:20070104
Mus musculus	MGI:95742 (score: 1.000; bootstrap: 82%)	From Inparanoid:20070104
Oryza gramene	Q6ES23 (score: 1.000; bootstrap: 100%) Q9XGF2 (score: 0.834) Q75GB0 (score: 0.722) Q6XC06 (score: 0.624) Q9ZWJ2 (score: 0.610) Q948T6 (score: 0.607) O04818 (score: 0.589)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000030963 (score: 1.000; bootstrap: 79%)	From Inparanoid:20070104
Rattus norvegicus	ENSRNOP00000000650 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YML004C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPBC12C22c (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000156322 (score: 1.000; bootstrap: 82%) NEWSINFRUP00000161532 (score: 0.601)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00022370001 (score: 1.000; bootstrap: 84%) GSTENP00003976001 (score: 0.182)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000023522 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	GLOA	From SHIGELLACYC
E. coli O157	GLOA	From ECOO157CYC
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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00903 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily
Superfamily (EcoliWiki Page)	SUPERFAMILY:54593
EcoCyc	EcoCyc:G6891	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG13421	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120003480	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB3197	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0005520	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 ^1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

gloA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to gloA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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