gloA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GloA

Synonyms

glyoxalase I, Ni-dependent[1], B1651[2][1], GloA[2][1] , ECK1647, JW1643, b1651

Product description

GloA[2][3];

Component of glyoxalase I[2][3]

Glyoxalase I, lactoylglutathione lyase, Ni(2+) cofactor; inactivated by zinc; dimeric[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0004462

lactoylglutathione lyase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004361

F

Seeded from EcoCyc (v14.0)

complete

GO:0004462

lactoylglutathione lyase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018146

F

Seeded from EcoCyc (v14.0)

complete

GO:0004462

lactoylglutathione lyase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:4.4.1.5

F

Seeded from EcoCyc (v14.0)

complete

GO:0016151

nickel ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0533

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of glyoxalase I

could be indirect

Protein

dnaK

PMID:15690043[5]

Experiment(s):EBI-884394, EBI-891329

Protein

creC

PMID:15690043[5]

Experiment(s):EBI-891329

Protein

groL

PMID:15690043[5]

Experiment(s):EBI-891329

Protein

pta

PMID:19402753[6]

LCMS(ID Probability):98.9

Protein

groL

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

groS

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

slyD

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

dnaE

PMID:19402753[6]

MALDI(Z-score):18.801583

Protein

dnaX

PMID:19402753[6]

MALDI(Z-score):23.842581

Protein

creC

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

speA

PMID:19402753[6]

MALDI(Z-score):21.143305

Protein

nikA

PMID:19402753[6]

MALDI(Z-score):25.024707

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MRLLHTMLRV GDLQRSIDFY TKVLGMKLLR TSENPEYKYS LAFVGYGPET EEAVIELTYN
WGVDKYELGT AYGHIALSVD NAAEACEKIR QNGGNVTREA GPVKGGTTVI AFVEDPDGYK
IELIEEKDAG RGLGN
Length

135

Mol. Wt

14.919 kDa

pI

4.8 (calculated)

Extinction coefficient

17,420 - 17,545 (calc based on 8 Y, 1 W, and 1 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

2..123

PF00903 Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

PMID:19920124[7]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=gloA taxon=562,83333 </beststructure>

Models

View models at:

</protect>

Structure figures

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</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129609

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946161

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005520

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AC81

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G6891

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13421

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946161

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003480

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3197

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  6. 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  7. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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