glnE:On One Page
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Quickview | | Gene | | Product(s) | | Expression| | Evolution | | References | |
Quickview
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| Standard Name |
glnE |
|---|---|
| Gene Synonym(s) |
ECK3043, b3053, JW3025[1], JW3025 |
| Product Desc. |
Protein adenylyltransferase, modifies glutamine synthase; catalyzes adenylation and deadenylation[4] |
| Product Synonyms(s) |
fused deadenylyltransferase[1], adenylyltransferase for glutamine synthetase[1], B3053[2][1], GlnE[2][1] , ECK3043, JW3025, b3053 |
| Function from GO |
<GO_nr /> |
| Knock-Out Phenotype | |
| Regulation/Expression | |
| Regulation/Activity | |
| Quick Links |
|
| edit table |
</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>
Notes
GlnE has two homologous domains: the N-terminal domain is the deadenylase, and the C-terminal domain is the adenylase (Jaggi, 1997).[4]
Gene
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
glnE |
|---|---|
| Mnemonic |
Glutamine |
| Synonyms |
ECK3043, b3053, JW3025[1], JW3025 |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
68.86 minutes, 68.86 minutes |
MG1655: 3197663..3194823 |
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NC_012967: 3132475..3129635 |
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NC_012759: 3081971..3084811 |
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|
W3110 |
|
W3110: 3198297..3195457 |
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DH10B: 3295408..3292568 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔglnE (Keio:JW3025) |
deletion |
deletion |
PMID:16738554 |
||||
|
glnE::Tn5KAN-I-SceI (FB21013) |
Insertion at nt 1520 in Plus orientation |
PMID:15262929 |
contains pKD46 | ||||
|
ΔglnE746::kan |
PMID:16738554 |
| |||||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
| edit table |
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3025 |
Plasmid clone |
PMID:16769691 Status:Clone OK Primer 1:GCCAAGCCGCTCTCTTCACCGTT Primer 2:CCTTCTTCCACCAGCCACTTCTG | |
|
Kohara Phage |
PMID:3038334 | ||
|
Linked marker |
est. P1 cotransduction: 55% [6] | ||
|
Linked marker |
est. P1 cotransduction: 47% [6] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc:EG11602 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG11602 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120001550 |
Escherichia coli str. K-12 substr. MG1655 | |
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
EchoBASE:EB1559 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0010018 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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Product(s)
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Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
GlnE |
|---|---|
| Synonyms |
fused deadenylyltransferase[1], adenylyltransferase for glutamine synthetase[1], B3053[2][1], GlnE[2][1] , ECK3043, JW3025, b3053 |
| Product description |
Protein adenylyltransferase, modifies glutamine synthase; catalyzes adenylation and deadenylation[4] |
| EC number (for enzymes) | |
| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
GO:0005737 |
cytoplasm |
C |
Seeded from Riley et al 2006 [1]. |
Missing: evidence, reference | ||||
|
GO:0000166 |
nucleotide binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0547 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0005524 |
ATP binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0067 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0008882 |
(glutamate-ammonia-ligase) adenylyltransferase activity |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
HAMAP:MF_00802 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0008882 |
(glutamate-ammonia-ligase) adenylyltransferase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR005190 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0008882 |
(glutamate-ammonia-ligase) adenylyltransferase activity |
GOA:spec |
IEA: Inferred from Electronic Annotation |
EC:2.7.7.42 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0016740 |
transferase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0808 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0016779 |
nucleotidyltransferase activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
InterPro:IPR013546 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
|
GO:0016779 |
nucleotidyltransferase activity |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
SP_KW:KW-0548 |
F |
Seeded from EcoCyc (v14.0) |
complete | |
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
eno |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
|
Protein |
rpsD |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
|
Protein |
ahpC |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
|
Protein |
dps |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
|
Protein |
glgP |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
|
Protein |
acnA |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
|
Protein |
tig |
PMID:19402753 |
LCMS(ID Probability):99.6 | |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MKPLSSPLQQ YWQTVVERLP EPLAEESLSA QAKSVLTFSD FVQDSVIAHP EWLTELESQP PQADEWQHYA AWLQEALCNV SDEAGLMREL RLFRRRIMVR IAWAQTLALV TEESILQQLS YLAETLIVAA RDWLYDACCR EWGTPCNAQG EAQPLLILGM GKLGGGELNF SSDIDLIFAW PEHGCTQGGR RELDNAQFFT RMGQRLIKVL DQPTQDGFVY RVDMRLRPFG ESGPLVLSFA ALEDYYQEQG RDWERYAMVK ARIMGDSEGV YANELRAMLR PFVFRRYIDF SVIQSLRNMK GMIAREVRRR GLTDNIKLGA GGIREIEFIV QVFQLIRGGR EPSLQSRSLL PTLSAIAELH LLSENDAEQL RVAYLFLRRL ENLLQSINDE QTQTLPSDEL NRARLAWAMD FADWPQLTGA LTAHMTNVRR VFNELIGDDE SETQEESLSE QWRELWQDAL QEDDTTPVLA HLSEDDRKQV LTLIADFRKE LDKRTIGPRG RQVLDHLMPH LLSDVCARED AAVTLSRITA LLVGIVTRTT YLELLSEFPA ALKHLISLCA ASPMIASQLA RYPLLLDELL DPNTLYQPTA TDAYRDELRQ YLLRVPEDDE EQQLEALRQF KQAQLLRIAA ADIAGTLPVM KVSDHLTWLA EAMIDAVVQQ AWVQMVARYG KPNHLNEREG RGFAVVGYGK LGGWELGYSS DLDLIFLHDC PMDAMTDGER EIDGRQFYLR LAQRIMHLFS TRTSSGILYE VDARLRPSGA AGMLVTSAEA FADYQKNEAW TWEHQALVRA RVVYGDPQLT AHFDAVRREI MTLPREGKTL QTEVREMREK MRAHLGNKHR DRFDIKADEG GITDIEFITQ YLVLRYAHEK PKLTRWSDNV RILELLAQND IMEEQEAMAL TRAYTTLRDE LHHLALQELP GHVSEDCFTA ERELVRASWQ KWLVEE |
| Length |
946 |
| Mol. Wt |
108.42 kDa |
| pI |
5.0 (calculated) |
| Extinction coefficient |
154,240 - 155,365 (calc based on 26 Y, 21 W, and 9 C residues) |
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Domains/Motifs/Modification Sites
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See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
ASAP:ABE-0010018 |
Escherichia coli str. K-12 substr. MG1655 | |
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
EcoCyc:EG11602 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG11602 |
Escherichia coli str. K-12 substr. MG1655 | |
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
RegulonDB:ECK120001550 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB1559 |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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Expression
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Overview
This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Cellular Levels
| Molecule | Organism or Strain | Value | Units | Experimental Conditions | Assay used | Notes | Reference(s) |
|---|---|---|---|---|---|---|---|
|
Protein |
E. coli K-12 MC4100 |
8.80E+01 |
molecules/cell |
|
emPAI |
PMID:18304323 | |
|
Protein |
Ecoli K-12 |
12.918+/-0.088 |
Molecules/cell |
|
Single Molecule Fluorescence |
PMID:20671182 | |
|
mRNA |
Ecoli K-12 |
0.036091549 |
Molecules/cell |
|
by RNA_Seq |
PMID:20671182 | |
|
Protein |
E. coli K-12 MG1655 |
212 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
|
Protein |
E. coli K-12 MG1655 |
153 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
|
Protein |
E. coli K-12 MG1655 |
180 |
molecules/cell/generation |
|
Ribosome Profiling |
PMID: 24766808 | |
| edit table |
Notes
Transcription and Transcriptional Regulation
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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.
|
 Figure courtesy of RegulonDB |
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Notes
This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Translation and Regulation of Translation
<protect><gbrowseImage>
name=NC_000913:3197643..3197683
source=MG1655
flip=1
type=Gene+DNA_+Protein
preset=Nterminus
</gbrowseImage>
This picture shows the sequence around the N-terminus.
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Notes
This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.
Turnover and Regulation of Turnover
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Notes
This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.
Experimental
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Mutations Affecting Expression
See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.
| Allele Name | Mutation | Phenotype | Reference |
|---|---|---|---|
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Expression Studies
See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.
| Type | Reference | Notes |
|---|---|---|
|
microarray |
NCBI GEO profiles for glnE | |
|
microarray |
Summary of data for glnE from multiple microarray studies | |
| edit table |
Expression Resources
See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.
| Resource Name | Resource Type | Description | Source | Notes |
|---|---|---|---|---|
| edit table |
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Notes
Accessions Related to glnE Expression
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc:EG11602 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB1559 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG11602 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120001550 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0010018 |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
Evolution
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Homologs in Other Organisms
See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.
| Organism | Homologs (Statistics) | Comments |
|---|---|---|
|
Xenopus tropicalis |
|
From Inparanoid:20070104 |
|
Shigella flexneri |
GLNE |
From SHIGELLACYC |
|
E. coli O157 |
GLNE |
From ECOO157CYC |
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Do-It-Yourself Web Tools
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Notes
Families
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc:EG11602 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene:EG11602 |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB:ECK120001550 |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE:EB1559 |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP:ABE-0010018 |
Escherichia coli str. K-12 substr. MG1655 | |
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
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