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On One Page

Quickview |

Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	glnA
Gene Synonym(s)	ECK3863, b3870, JW3841, G7966^[1]^[2]
Product Desc.	adenylyl-[glutamine synthetase]^[2]^[3], GlnA^[2]^[3]; Component of glutamine synthetase^[2]^[3] Glutamine synthase^[4]
Product Synonyms(s)	glutamine synthetase^[1], B3870^[2]^[1], adenylyl-[L-glutamate:ammonia Ligase (ADP-forming)]^[2]^[1], adenylyl-GS^[2]^[1] , ECK3863, JW3841, b3870
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): glnA^[2], glnALG^[2], OP00034
Regulation/Activity
Quick Links
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Notes

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Induced during biofilm formation. Mutants have reduced OM vesiculation (McBroom, 2006).^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	glnA
Mnemonic	Glutamine
Synonyms	ECK3863, b3870, JW3841, G7966^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	87.39 minutes	MG1655: 4056057..4054648 <gbrowseImage> name=NC_000913:4054648..4056057 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4036292..4034883 <gbrowseImage> name=NC_012967:4034883..4036292 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3944317..3945726 <gbrowseImage> name=NC_012759:3944317..3945726 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3578647..3580056 <gbrowseImage> name=NC_007779:3578647..3580056 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4154977..4153568 <gbrowseImage> name=NC_010473:4153568..4154977 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	4054651	Edman degradation	PMID:9298646 PMID:9740056
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔglnA (Keio:JW3841)	deletion	deletion	PMID:16738554	Shigen CGSC10775^[5]
glnA2				CGSC:6543
glnA3				CGSC:6547
glnA202				CGSC:9548
ΔglnA732::kan			PMID:16738554	CGSC:101154
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3841	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCCGCTGAACACGTACTGAC Primer 2:CCGACGCTGTAGTACAGCTCAAA
10F1	Kohara Phage	Genobase	PMID:3038334
8D12	Kohara Phage	Genobase	PMID:3038334
fad-751::Tn10	Linked marker	CAG18496 = CGSC7465^[5]	est. P1 cotransduction: 35% ^[6] Synonyms:fadAB101::Tn10
zih-35::Tn10	Linked marker	CAG18495 = CGSC7467^[5]	est. P1 cotransduction: 72% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10383	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10383	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000376	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948370	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0378	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012640	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	GlnA
Synonyms	glutamine synthetase^[1], B3870^[2]^[1], adenylyl-[L-glutamate:ammonia Ligase (ADP-forming)]^[2]^[1], adenylyl-GS^[2]^[1] , ECK3863, JW3841, b3870
Product description	adenylyl-[glutamine synthetase]^[2]^[3], GlnA^[2]^[3]; Component of glutamine synthetase^[2]^[3] Glutamine synthase^[4]
EC number (for enzymes)	6.3.1.2^[1]
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Notes

Function

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<protect> Gene Ontology

See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000166	nucleotide binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0547	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004356	glutamate-ammonia ligase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001637	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004356	glutamate-ammonia ligase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004809	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004356	glutamate-ammonia ligase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008146	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004356	glutamate-ammonia ligase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008147	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004356	glutamate-ammonia ligase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:6.3.1.2	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005524	ATP binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0067	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005829	cytosol	PMID:16858726	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
	GO:0006542	glutamine biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008147	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009399	nitrogen fixation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004809	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	PMID:16858726	IDA: Inferred from Direct Assay		C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of glutamine synthetase	could be indirect
Protein	rplB		PMID:16606699	Experiment(s):EBI-1146848
Protein	rplP		PMID:16606699	Experiment(s):EBI-1146848
Protein	rplQ		PMID:16606699	Experiment(s):EBI-1146848
Protein	rpsC		PMID:16606699	Experiment(s):EBI-1146848
Protein	ycgE		PMID:16606699	Experiment(s):EBI-1146848
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Notes

Glutamine Synthetase GlnA regulates cytoplasmic nitrogen levels. It catalyzes the reaction between glutamine and oxoglutarate to form 2 molecules of glutamate. The reaction is energetically expensive and consumes one ATP per reaction. The enzyme catalyzes the final step of ammonia fixation. Glutamine synthetase is thought to interact with P_II proteins and regulates ammonia fixation rate in other bacteria.

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSAEHVLTML NEHEVKFVDL RFTDTKGKEQ HVTIPAHQVN AEFFEEGKMF DGSSIGGWKG INESDMVLMP DASTAVIDPF FADSTLIIRC DILEPGTLQG YDRDPRSIAK RAEDYLRSTG IADTVLFGPE PEFFLFDDIR FGSSISGSHV AIDDIEGAWN SSTQYEGGNK GHRPAVKGGY FPVPPVDSAQ DIRSEMCLVM EQMGLVVEAH HHEVATAGQN EVATRFNTMT KKADEIQIYK YVVHNVAHRF GKTATFMPKP MFGDNGSGMH CHMSLSKNGV NLFAGDKYAG LSEQALYYIG GVIKHAKAIN ALANPTTNSY KRLVPGYEAP VMLAYSARNR SASIRIPVVS SPKARRIEVR FPDPAANPYL CFAALLMAGL DGIKNKIHPG EAMDKNLYDL PPEEAKEIPQ VAGSLEEALN ELDLDREFLK AGGVFTDEAI DAYIALRREE DDRVRMTPHP VEFELYYSV
Length	469
Mol. Wt	51.904 kDa
pI	5.3 (calculated)
Extinction coefficient	36,330 - 36,830 (calc based on 17 Y, 2 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P0A9C5
Domain	102..379	PF00120 Glutamine synthetase, catalytic domain		PMID:19920124
Domain	14..95	PF03951 Glutamine synthetase, beta-Grasp domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=glnA taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131710	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948370	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012640	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0A9C5	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10383	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10383	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948370	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000376	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0378	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	7.65E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	E. coli K-12 EMG2	420	molecules/cell	Medium: MOPS glucose minimal Temperature (°C): 37 Doubling time (min): Growth phase: Exponential OD: OD600 = 0.2 to 0.25 other: aerated	Quantitative protein sequencing	Spot ID: M27	PMID: 9298646
Protein	E. coli K-12 EMG2	1040	molecules/cell	Medium: MOPS glucose minimal Temperature (°C): 37 Doubling time (min): Growth phase: Exponential OD: OD600 = 0.2 to 0.25 other: aerated	Quantitative protein sequencing	Spot ID: M28	PMID: 9298646
Protein	E. coli K-12 MG1655	2480	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	14311	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1186	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
glnA
glnALG
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4056037..4056077 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3870 (EcoliWiki Page)	NCBI GEO profiles for glnA
microarray	GenExpDB:b3870 (EcoliWiki Page)	Summary of data for glnA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4055543..4056017) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ20; Well:E2^[7]
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Notes

Accessions Related to glnA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10383	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0378	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3870
EcoGene	EcoGene:EG10383	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000376	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012640	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000019490 (score: 1.000; bootstrap: 100%) ENSANGP00000014914 (score: 0.575)	From Inparanoid:20070104
Apis mellifera	ENSAPMP00000015737 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT3G53180 (score: 1.000; bootstrap: 96%)	From Inparanoid:20070104
Caenorhabditis briggsae	WBGene00039586 (score: 1.000; bootstrap: 100%) WBGene00039664 (score: 1.000; bootstrap: 100%) WBGene00039019 (score: 0.806) WBGene00034517 (score: 0.579) WBGene00035212 (score: 0.463)	From Inparanoid:20070104
Caenorhabditis elegans	WBGene00001607 (score: 1.000; bootstrap: 100%) WBGene00001606 (score: 1.000; bootstrap: 100%) WBGene00001603 (score: 0.806) WBGene00001604 (score: 0.582) WBGene00001602 (score: 0.426)	From Inparanoid:20070104
Ciona intestinalis	ENSCINP00000011993 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Danio rerio	ZDB-CDNA-040425-339 (score: 1.000; bootstrap: 100%) ZDB-GENE-030131-8417 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040425-340 (score: 1.000; bootstrap: 100%) ZDB-CDNA-040425-338 (score: 0.897) ZDB-GENE-030131-688 (score: 0.893) ZDB-CDNA-041212-74 (score: 0.632) ZDB-GENE-041212-65 (score: 0.632)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000013857 (score: 1.000; bootstrap: 100%) ENSGALP00000005819 (score: 0.577)	From Inparanoid:20070104
Oryza gramene	Q5MD07 (score: 1.000; bootstrap: 97%) Q7XDQ4 (score: 0.808)	From Inparanoid:20070104
Saccharomyces cerevisiae	YPR035W (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Takifugu rubripes	NEWSINFRUP00000137325 (score: 1.000; bootstrap: 100%) NEWSINFRUP00000151139 (score: 0.727)	From Inparanoid:20070104
Shigella flexneri	GLNA	From SHIGELLACYC
E. coli O157	GLNA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00120 Glutamine synthetase, catalytic domain
Pfam (EcoliWiki Page)	PF03951 Glutamine synthetase, beta-Grasp domain
Panther (EcoliWiki Page)	PTHR20852:SF7
Superfamily (EcoliWiki Page)	SUPERFAMILY:54368
Superfamily (EcoliWiki Page)	SUPERFAMILY:55931
EcoCyc	EcoCyc:EG10383	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10383	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000376	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0378	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012640	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 ^2.15 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

glnA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to glnA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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