glnA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glnA

Mnemonic

Glutamine

Synonyms

ECK3863, b3870, JW3841, G7966[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

87.39 minutes 

MG1655: 4056057..4054648
<gbrowseImage> name=NC_000913:4054648..4056057 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4036292..4034883
<gbrowseImage> name=NC_012967:4034883..4036292 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3944317..3945726
<gbrowseImage> name=NC_012759:3944317..3945726 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3578647..3580056
<gbrowseImage> name=NC_007779:3578647..3580056 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4154977..4153568
<gbrowseImage> name=NC_010473:4153568..4154977 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4054651

Edman degradation

PMID:9298646[3]
PMID:9740056[4]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔglnA (Keio:JW3841)

deletion

deletion

PMID:16738554[5]

Shigen
CGSC10775[6]

glnA2

CGSC:6543

glnA3

CGSC:6547

glnA202

CGSC:9548

ΔglnA732::kan

PMID:16738554[5]

CGSC:101154


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3841

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCTCCGCTGAACACGTACTGAC

Primer 2:CCGACGCTGTAGTACAGCTCAAA

10F1

Kohara Phage

Genobase

PMID:3038334[8]

8D12

Kohara Phage

Genobase

PMID:3038334[8]

fad-751::Tn10

Linked marker

CAG18496 = CGSC7465[6]

est. P1 cotransduction: 35% [9]
Synonyms:fadAB101::Tn10

zih-35::Tn10

Linked marker

CAG18495 = CGSC7467[6]

est. P1 cotransduction: 72% [9]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10383

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10383

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000376

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948370

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0378

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012640

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. Tonella, L et al. (1998) '98 Escherichia coli SWISS-2DPAGE database update. Electrophoresis 19 1960-71 PubMed
  5. 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  6. 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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