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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

Quickview

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Standard Name	glmM
Gene Synonym(s)	ECK3165, b3176, JW3143, mrsA, yhbF^[1], yhbF
Product Desc.	Phosphoglucosamine mutase; UDP-GlcNAc pathway, peptidoglycan, lipopolysaccharide synthesis; mRNA stability effects^[2]
Product Synonyms(s)	phosphoglucosamine mutase^[1], B3176^[3]^[1], YhbF^[3]^[1], GlmM^[3]^[1], MrsA^[3]^[1] , ECK3165, JW3143, mrsA, yhbF, b3176
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): glmM^[3]
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Autophosphorylated enzyme (at Ser-102) is active form. glmM is an essential gene. Binds TrxA (Kumar, 2004).^[2]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	glmM
Mnemonic	Glucosamine
Synonyms	ECK3165, b3176, JW3143, mrsA, yhbF^[1], yhbF
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	71.57 minutes	MG1655: 3322092..3320755 <gbrowseImage> name=NC_000913:3320755..3322092 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3259304..3257967 <gbrowseImage> name=NC_012967:3257967..3259304 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3207903..3209240 <gbrowseImage> name=NC_012759:3207903..3209240 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3323925..3322588 <gbrowseImage> name=NC_007779:3322588..3323925 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3419837..3418500 <gbrowseImage> name=NC_010473:3418500..3419837 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3320758	Edman degradation	PMID:8550580
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔglmM (Keio:JW3143)	deletion	deletion		PMID:16738554	Shigen CGSC10388^[4]
glmMS100A		S100A	2% of wild-type activity			seeded from UniProt:P31120
glmMS100T		S100T	20-fold increase in the non- specific phosphoglucomutase activity towards glucose-phosphate substrates (non aminated)			seeded from UniProt:P31120
glmMS102A		S102A	Loss of activity in the absence or presence of glucosamine-1,6-diP			seeded from UniProt:P31120
ΔglmM784::kan				PMID:16738554	CGSC:101152
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Notes

The Keio collection^[5] lists a deletion of glmM. The insertion in this strain is a duplication of the glmM region.^[6]

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3143	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAGTAATCGTAAATATTTCGG Primer 2:CCAACGGCTTTTACTGCATCGGC
E4G11S	Kohara Phage	Genobase	PMID:3038334
18H7	Kohara Phage	Genobase	PMID:3038334
14F11	Kohara Phage	Genobase	PMID:3038334
aer-3075::Tn10	Linked marker	CAG12152 = CGSC7438^[4]	est. P1 cotransduction: % ^[7] Synonyms:zgh-3075::Tn10, zgj-3075::Tn10
sfsB203::Tn10	Linked marker	CAG12072 = CGSC7440^[4]	est. P1 cotransduction: 70% ^[7] Synonyms:zgj-203::Tn10, zha-203::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11553	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11553	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001506	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947692	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1514	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010439	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	GlmM
Synonyms	phosphoglucosamine mutase^[1], B3176^[3]^[1], YhbF^[3]^[1], GlmM^[3]^[1], MrsA^[3]^[1] , ECK3165, JW3143, mrsA, yhbF, b3176
Product description	Phosphoglucosamine mutase; UDP-GlcNAc pathway, peptidoglycan, lipopolysaccharide synthesis; mRNA stability effects^[2]
EC number (for enzymes)	5.4.2.10^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0000287	magnesium ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006352	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000287	magnesium ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR016066	F	Seeded from EcoCyc (v14.0)	complete
	GO:0000287	magnesium ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0460	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008966	phosphoglucosamine mutase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_01554	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008966	phosphoglucosamine mutase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006352	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008966	phosphoglucosamine mutase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:5.4.2.10	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	murC	PMID:15690043	Experiment(s):EBI-888671
Protein	recA	PMID:15690043	Experiment(s):EBI-888671
Protein	tufA	PMID:15690043	Experiment(s):EBI-888671
Protein	nusG	PMID:15690043	Experiment(s):EBI-893878
Protein	rplK	PMID:15690043	Experiment(s):EBI-893878
Protein	rplL	PMID:15690043	Experiment(s):EBI-893878
Protein	rplM	PMID:15690043	Experiment(s):EBI-893878
Protein	rplV	PMID:15690043	Experiment(s):EBI-893878
Protein	rpsB	PMID:15690043	Experiment(s):EBI-893878
Protein	rpsE	PMID:15690043	Experiment(s):EBI-893878
Protein	rpsJ	PMID:15690043	Experiment(s):EBI-893878
Protein	mscS	PMID:15690043	Experiment(s):EBI-893878
Protein	mscS	PMID:19402753	LCMS(ID Probability):99.0
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSNRKYFGTD GIRGRVGDAP ITPDFVLKLG WAAGKVLARH GSRKIIIGKD TRISGYMLES ALEAGLAAAG LSALFTGPMP TPAVAYLTRT FRAEAGIVIS ASHNPFYDNG IKFFSIDGTK LPDAVEEAIE AEMEKEISCV DSAELGKASR IVDAAGRYIE FCKATFPNEL SLSELKIVVD CANGATYHIA PNVLRELGAN VIAIGCEPNG VNINAEVGAT DVRALQARVL AEKADLGIAF DGDGDRVIMV DHEGNKVDGD QIMYIIAREG LRQGQLRGGA VGTLMSNMGL ELALKQLGIP FARAKVGDRY VLEKMQEKGW RIGAENSGHV ILLDKTTTGD GIVAGLQVLA AMARNHMSLH DLCSGMKMFP QILVNVRYTA GSGDPLEHES VKAVTAEVEA ALGNRGRVLL RKSGTEPLIR VMVEGEDEAQ VTEFAHRIAD AVKAV
Length	445
Mol. Wt	47.544 kDa
pI	5.9 (calculated)
Extinction coefficient	24,410 - 25,035 (calc based on 9 Y, 2 W, and 5 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P31120
Modification Site	102	phosphorylation site at S102	probability greater than 75%	PMID:17938405
Domain	373..443	PF00408 Phosphoglucomutase/phosphomannomutase, C-terminal domain		PMID:19920124
Domain	4..138	PF02878 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I		PMID:19920124
Domain	156..254	PF02879 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II		PMID:19920124
Domain	258..369	PF02880 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=glmM taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131066	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947692	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010439	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P31120	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11553	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11553	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947692	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001506	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1514	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.04E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	146.558+/-2.554	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
Protein	E. coli K-12 MG1655	5713	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1268	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	3253	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
glmM
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3322072..3322112 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3176 (EcoliWiki Page)	NCBI GEO profiles for glmM
microarray	GenExpDB:b3176 (EcoliWiki Page)	Summary of data for glmM from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to glmM Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11553	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1514	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3176
EcoGene	EcoGene:EG11553	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001506	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010439	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Bos taurus	ENSBTAP00000000902 (score: 1.000; bootstrap: 51%)	From Inparanoid:20070104
Canis familiaris	ENSCAFP00000004345 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Dictyostelium discoideum	DDB0204675 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0036298 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila pseudoobscura	GA10449-PA (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Gallus gallus	ENSGALP00000025512 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Macaca mulatta	ENSMMUP00000016524 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YEL058W (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC12961c (score: 1.000; bootstrap: 100%) SPAC13C55c (score: 0.299)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000005021 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	MRSA	From SHIGELLACYC
E. coli O157	MRSA	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Panther (EcoliWiki Page)	PTHR22573:SF14
Superfamily (EcoliWiki Page)	SUPERFAMILY:53738
Superfamily (EcoliWiki Page)	SUPERFAMILY:53738
Superfamily (EcoliWiki Page)	SUPERFAMILY:53738
Superfamily (EcoliWiki Page)	SUPERFAMILY:55957
Pfam (EcoliWiki Page)	PF00408 Phosphoglucomutase/phosphomannomutase, C-terminal domain
Pfam (EcoliWiki Page)	PF02878 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain I
Pfam (EcoliWiki Page)	PF02879 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II
Pfam (EcoliWiki Page)	PF02880 Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain III
EcoCyc	EcoCyc:EG11553	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11553	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001506	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1514	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010439	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 ^3.6 ^3.7 ^3.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
↑ Yamamoto, N et al. (2009) Update on the Keio collection of Escherichia coli single-gene deletion mutants. Mol. Syst. Biol. 5 335 PubMed
↑ ^7.0 ^7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

glmM:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to glmM Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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