glgC:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

glgC

Gene Synonym(s)

ECK3416, b3430, JW3393[1], JW3393

Product Desc.

GlgC[2][3];

Component of glucose-1-phosphate adenylyltransferase[2][3]

Glucose-1-phosphate adenylyltransferase; ADP-glucose pyrophosphorylase[4]

Product Synonyms(s)

glucose-1-phosphate adenylyltransferase[1], B3430[2][1], GlgC[2][1] , ECK3416, JW3393, b3430

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): glgCAP[2], glgC, glgCAY, OP00164

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

glgC

Mnemonic

Glycogen

Synonyms

ECK3416, b3430, JW3393[1], JW3393

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

76.86 minutes 

MG1655: 3567351..3566056
<gbrowseImage> name=NC_000913:3566056..3567351 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3500803..3499487
<gbrowseImage> name=NC_012967:3499487..3500803 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3454550..3455845
<gbrowseImage> name=NC_012759:3454550..3455845 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4071087..4072382
<gbrowseImage> name=NC_007779:4071087..4072382 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3665096..3663801
<gbrowseImage> name=NC_010473:3663801..3665096 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3566059

Edman degradation

PMID:359552


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔglgC (Keio:JW3393)

deletion

deletion

PMID:16738554

Shigen
CGSC10526[5]

glgC::Tn5KAN-I-SceI (FB21199)

Insertion at nt 940 in Minus orientation

PMID:15262929

E. coli Genome Project:FB21199

does not contain pKD46

glgC::Tn5KAN-I-SceI (FB21200)

Insertion at nt 940 in Minus orientation

PMID:15262929

E. coli Genome Project:FB21200

contains pKD46

glgCA44T

A44T

(in SG14 mutant; lower apparent affinities for substrates, the activator, F16BP and the inhibitor)

Strain variation; seeded from UniProt:P0A6V1

glgCR67C

R67C

(in CL1136 mutant; less dependent on the allosteric activator, F16BP, for activity and less sensitive to inhibition by AMP)

Strain variation; seeded from UniProt:P0A6V1

glgCP295S

P295S

(in SG5 mutant)

Strain variation; seeded from UniProt:P0A6V1

glgCG336D

G336D

(in 618 mutant; causes lowered affinity for AMP)

Strain variation; seeded from UniProt:P0A6V1

glgCK195E,I,H,Q,R

K195E,I,H,Q,R

No loss in enzyme activity

seeded from UniProt:P0A6V1

glgC3

CGSC:11086

glgC16

CGSC:85259

ΔglgC763::kan

PMID:16738554

CGSC:101142


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3393

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGTTAGTTTAGAGAAGAACGA

Primer 2:CCTCGCTCCTGTTTATGCCCTAA

16A5

Kohara Phage

Genobase

PMID:3038334

E5B4

Kohara Phage

Genobase

PMID:3038334

glgP721::Tn10

Linked marker

CAG18638 = CGSC7451[5]

est. P1 cotransduction: 91% [6]
Synonyms:zhg-3086::Tn10, zhh-21::Tn10

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[5]

est. P1 cotransduction: 39% [6]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10379

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10379

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000372

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947942

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0374

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011204

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GlgC

Synonyms

glucose-1-phosphate adenylyltransferase[1], B3430[2][1], GlgC[2][1] , ECK3416, JW3393, b3430

Product description

GlgC[2][3];

Component of glucose-1-phosphate adenylyltransferase[2][3]

Glucose-1-phosphate adenylyltransferase; ADP-glucose pyrophosphorylase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0000271

polysaccharide biosynthetic process

P

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005978

glycogen biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00624

P

Seeded from EcoCyc (v14.0)

complete

GO:0005978

glycogen biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005836

P

Seeded from EcoCyc (v14.0)

complete

GO:0005978

glycogen biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0320

P

Seeded from EcoCyc (v14.0)

complete

GO:0008878

glucose-1-phosphate adenylyltransferase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00624

F

Seeded from EcoCyc (v14.0)

complete

GO:0008878

glucose-1-phosphate adenylyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011831

F

Seeded from EcoCyc (v14.0)

complete

GO:0008878

glucose-1-phosphate adenylyltransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.7.27

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of glucose-1-phosphate adenylyltransferase

could be indirect

Protein

ytfN

PMID:16606699

Experiment(s):EBI-1145875
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MVSLEKNDHL MLARQLPLKS VALILAGGRG TRLKDLTNKR AKPAVHFGGK FRIIDFALSN
CINSGIRRMG VITQYQSHTL VQHIQRGWSF FNEEMNEFVD LLPAQQRMKG ENWYRGTADA
VTQNLDIIRR YKAEYVVILA GDHIYKQDYS RMLIDHVEKG ARCTVACMPV PIEEASAFGV
MAVDENDKII EFVEKPANPP SMPNDPSKSL ASMGIYVFDA DYLYELLEED DRDENSSHDF
GKDLIPKITE AGLAYAHPFP LSCVQSDPDA EPYWRDVGTL EAYWKANLDL ASVVPELDMY
DRNWPIRTYN ESLPPAKFVQ DRSGSHGMTL NSLVSGGCVI SGSVVVQSVL FSRVRVNSFC
NIDSAVLLPE VWVGRSCRLR RCVIDRACVI PEGMVIGENA EEDARRFYRS EEGIVLVTRE
MLRKLGHKQE R
Length

431

Mol. Wt

48.697 kDa

pI

6.1 (calculated)

Extinction coefficient

55,350 - 56,475 (calc based on 15 Y, 6 W, and 9 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A6V1

Domain

21..293

PF00483 Nucleotidyl transferase

PMID:19920124

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=glgC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131304

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947942

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011204

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6V1

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10379

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10379

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947942

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000372

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0374

Escherichia coli str. K-12 substr. MG1655

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Notes

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

Ecoli K-12

1.458+/-0.162

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.155598456

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

257

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

910

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

514

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

glgCAP

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3567331..3567371 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3430 (EcoliWiki Page)

NCBI GEO profiles for glgC

microarray

GenExpDB:b3430 (EcoliWiki Page)

Summary of data for glgC from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to glgC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10379

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0374

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3430

EcoGene

EcoGene:EG10379

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000372

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011204

Escherichia coli str. K-12 substr. MG1655

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000003742 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000003549 (score: 1.000; bootstrap: 100%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT5G48300 (score: 1.000; bootstrap: 100%)
  • AT5G19220 (score: 0.352)
  • AT1G27680 (score: 0.343)
  • AT2G21590 (score: 0.299)
  • AT4G39210 (score: 0.290)
  • AT1G05610 (score: 0.212)

From Inparanoid:20070104

Oryza gramene

  • Q7EZW3 (score: 1.000; bootstrap: 99%)
  • P55238 (score: 0.861)
  • Q4L1B1 (score: 0.858)
  • Q947B9 (score: 0.852)
  • Q84QT8 (score: 0.810)
  • P15280 (score: 0.793)
  • Q69T99 (score: 0.793)
  • Q9ARH9 (score: 0.789)
  • Q8HS72 (score: 0.783)
  • Q9M4Z1 (score: 0.780)
  • Q941P2 (score: 0.780)
  • P30523 (score: 0.774)
  • Q4L1B2 (score: 0.774)
  • Q5XXD1 (score: 0.769)
  • Q947C0 (score: 0.762)
  • Q84JD3 (score: 0.685)
  • Q84J79 (score: 0.685)
  • Q84PF3 (score: 0.684)
  • Q84J86 (score: 0.684)
  • Q84PF5 (score: 0.675)
  • Q84PF4 (score: 0.674)
  • Q6AVT2 (score: 0.367)
  • O04896 (score: 0.354)
  • Q8GRM4 (score: 0.307)
  • Q7G065 (score: 0.265)
  • Q5VNT5 (score: 0.265)
  • P93430 (score: 0.265)
  • P12299 (score: 0.263)
  • P30524 (score: 0.263)
  • Q9ARI0 (score: 0.260)
  • Q688T8 (score: 0.259)
  • O23809 (score: 0.259)
  • Q7XJA9 (score: 0.254)
  • Q9XHV4 (score: 0.253)
  • P55241 (score: 0.247)
  • O48877 (score: 0.244)
  • P12300 (score: 0.238)
  • P55234 (score: 0.216)
  • O24224 (score: 0.196)
  • Q7X9S8 (score: 0.167)
  • Q4U131 (score: 0.121)
  • Q4U128 (score: 0.121)
  • Q4U168 (score: 0.120)
  • Q4U129 (score: 0.119)
  • Q4U162 (score: 0.118)
  • Q4U133 (score: 0.118)
  • Q4U179 (score: 0.116)
  • Q4U156 (score: 0.116)
  • Q4U140 (score: 0.116)
  • Q4U138 (score: 0.116)
  • Q4U137 (score: 0.116)
  • Q4U135 (score: 0.116)
  • P12298 (score: 0.115)
  • Q84JA5 (score: 0.109)
  • Q84P33 (score: 0.106)
  • Q84P35 (score: 0.105)
  • Q84P32 (score: 0.105)
  • Q84P34 (score: 0.097)
  • Q4U130 (score: 0.082)
  • O22593 (score: 0.064)

From Inparanoid:20070104

Shigella flexneri

GLGC

From SHIGELLACYC

E. coli O157

GLGC

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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Database Accession Notes

Pfam (EcoliWiki Page)

PF00483 Nucleotidyl transferase

Superfamily (EcoliWiki Page)

SUPERFAMILY:51161

Panther (EcoliWiki Page)

PTHR22572:SF10

Superfamily (EcoliWiki Page)

SUPERFAMILY:53448

EcoCyc

EcoCyc:EG10379

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10379

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000372

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0374

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011204

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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