galP:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

galP

Gene Synonym(s)

ECK2938, b2943, JW2910, Pgal[1], Pgal

Product Desc.

GalP - galactose MFS transporter[2][3]

D-Galactose/H+ symporter[4]

Product Synonyms(s)

D-galactose transporter[1], B2943[2][1], GalP[2][1] , ECK2938, JW2910, Pgal, b2943

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): galP[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Major Facilitator Superfamily (MFS). N-terminal Met is cleaved, as verified by mass spec.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

galP

Mnemonic

Galactose

Synonyms

ECK2938, b2943, JW2910, Pgal[1], Pgal

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

66.52 minutes 

MG1655: 3086306..3087700
<gbrowseImage> name=NC_000913:3086306..3087700 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2974035..2975429
<gbrowseImage> name=NC_012967:2974035..2975429 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2973454..2974848
<gbrowseImage> name=NC_012759:2973454..2974848 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3086940..3088334
<gbrowseImage> name=NC_007779:3086940..3088334 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Strain DH10B

V326A

Nonsynonomous mutation

PMID:18245285

ASAP

This is a SNP in E. coli K-12 Strain DH10B

ΔgalP (Keio:JW2910)

deletion

deletion

PMID:16738554

Shigen
CGSC10251[5]

galP::Tn5KAN-I-SceI (FB20987)

Insertion at nt 1002 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20987

contains pKD46

galP::Tn5KAN-I-SceI (FB20988)

Insertion at nt 1002 in Plus orientation

PMID:15262929

E. coli Genome Project:FB20988

does not contain pKD46

galP63

CGSC:5279

galP64

CGSC:8976

galP79

CGSC:10654

galP77::Tn10

CGSC:10770

ΔgalP789::kan

PMID:16738554

CGSC:101082


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2910

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCCCTGACGCTAAAAAACAGGG

Primer 2:CCATCGTGAGCGCCTATTTCGCG

23G4S

Kohara Phage

Genobase

PMID:3038334

12C6

Kohara Phage

Genobase

PMID:3038334

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[5]

est. P1 cotransduction: 97% [6]
Synonyms:zgd-210::Tn10, zgf-210::Tn10

nupG511::Tn10

Linked marker

CAG18472 = CGSC7433[5]

est. P1 cotransduction: 53% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12148

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12148

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002048

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947434

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2068

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009654

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GalP

Synonyms

D-galactose transporter[1], B2943[2][1], GalP[2][1] , ECK2938, JW2910, Pgal, b2943

Product description

GalP - galactose MFS transporter[2][3]

D-Galactose/H+ symporter[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0005215

transporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005828

F

Seeded from EcoCyc (v14.0)

complete

GO:0005215

transporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005829

F

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005828

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005829

P

Seeded from EcoCyc (v14.0)

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0008643

carbohydrate transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0762

P

Seeded from EcoCyc (v14.0)

complete

GO:0015293

symporter activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0769

F

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003663

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005829

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005828

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

GO:0022891

substrate-specific transmembrane transporter activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003663

F

Seeded from EcoCyc (v14.0)

complete

GO:0055085

transmembrane transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003663

P

Seeded from EcoCyc (v14.0)

complete

GO:0055085

transmembrane transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005828

P

Seeded from EcoCyc (v14.0)

complete

GO:0055085

transmembrane transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005829

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 12 predicted transmembrane domains

Daley et al. (2005) [7]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MPDAKKQGRS NKAMTFFVCF LAALAGLLFG LDIGVIAGAL PFIADEFQIT SHTQEWVVSS
MMFGAAVGAV GSGWLSFKLG RKKSLMIGAI LFVAGSLFSA AAPNVEVLIL SRVLLGLAVG
VASYTAPLYL SEIAPEKIRG SMISMYQLMI TIGILGAYLS DTAFSYTGAW RWMLGVIIIP
AILLLIGVFF LPDSPRWFAA KRRFVDAERV LLRLRDTSAE AKRELDEIRE SLQVKQSGWA
LFKENSNFRR AVFLGVLLQV MQQFTGMNVI MYYAPKIFEL AGYTNTTEQM WGTVIVGLTN
VLATFIAIGL VDRWGRKPTL TLGFLVMAAG MGVLGTMMHI GIHSPSAQYF AIAMLLMFIV
GFAMSAGPLI WVLCSEIQPL KGRDFGITCS TATNWIANMI VGATFLTMLN TLGNANTFWV
YAALNVLFIL LTLWLVPETK HVSLEHIERN LMKGRKLREI GAHD
Length

464

Mol. Wt

50.984 kDa

pI

9.8 (calculated)

Extinction coefficient

80,900 - 81,275 (calc based on 10 Y, 12 W, and 3 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

18..451

PF00083 Sugar (and other) transporter

PMID:19920124

<motif_map/>

tmhmm.php?gene=galP&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=galP taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130844

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947434

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009654

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AEP1

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG12148

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12148

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947434

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002048

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2068

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

368

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

194

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

151

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

galP

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3086286..3086326 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b2943 (EcoliWiki Page)

NCBI GEO profiles for galP

microarray

GenExpDB:b2943 (EcoliWiki Page)

Summary of data for galP from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to galP Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12148

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2068

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2943

EcoGene

EcoGene:EG12148

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002048

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009654

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000022770 (score: 1.000; bootstrap: 84%)
  • ENSANGP00000019756 (score: 0.101)
  • ENSANGP00000023240 (score: 0.088)
  • ENSANGP00000018204 (score: 0.088)
  • ENSANGP00000023250 (score: 0.086)
  • ENSANGP00000012244 (score: 0.064)
  • ENSANGP00000013880 (score: 0.060)
  • ENSANGP00000009437 (score: 0.058)
  • ENSANGP00000017944 (score: 0.053)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000006624 (score: 1.000; bootstrap: 95%)
  • ENSAPMP00000028471 (score: 0.121)
  • ENSAPMP00000016859 (score: 0.096)
  • ENSAPMP00000029432 (score: 0.088)
  • ENSAPMP00000004279 (score: 0.058)
  • ENSAPMP00000028567 (score: 0.054)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT1G30220 (score: 1.000; bootstrap: 91%)
  • AT4G16480 (score: 0.458)
  • AT2G35740 (score: 0.414)
  • AT2G43330 (score: 0.293)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000025291 (score: 1.000; bootstrap: 90%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00031299 (score: 1.000; bootstrap: 92%)
  • WBGene00033784 (score: 0.453)
  • WBGene00033785 (score: 0.165)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00010811 (score: 1.000; bootstrap: 94%)
  • WBGene00013073 (score: 0.492)
  • WBGene00013074 (score: 0.459)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000014687 (score: 1.000; bootstrap: 56%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000002429 (score: 1.000; bootstrap: 81%)
  • ENSCINP00000006944 (score: 0.677)

From Inparanoid:20070104

Danio rerio

  • ZDB-GENE-030829-25 (score: 1.000; bootstrap: 71%)
  • ZDB-CDNA-040425-3631 (score: 1.000; bootstrap: 72%)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0205325 (score: 1.000; bootstrap: 64%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0033644 (score: 1.000; bootstrap: 95%)
  • FBgn0050035 (score: 0.811)
  • FBgn0036316 (score: 0.159)
  • FBgn0034247 (score: 0.120)
  • FBgn0028560 (score: 0.112)
  • FBgn0037386 (score: 0.084)
  • FBgn0037387 (score: 0.072)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA15593-PA (score: 1.000; bootstrap: 96%)
  • GA11381-PA (score: 0.065)
  • GA19628-PA (score: 0.054)
  • GA12538-PA (score: 0.052)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000022613 (score: 1.000; bootstrap: 98%)
  • ENSGALP00000001249 (score: 1.000; bootstrap: 90%)
  • ENSGALP00000007155 (score: 0.133)
  • ENSGALP00000004710 (score: 0.105)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000280871 (score: 1.000; bootstrap: 87%)
  • ENSP00000275230 (score: 1.000; bootstrap: 84%)
  • ENSP00000352216 (score: 0.085)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000013178 (score: 1.000; bootstrap: 94%)
  • ENSMMUP00000030186 (score: 1.000; bootstrap: 93%)
  • ENSMMUP00000022258 (score: 0.089)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000021849 (score: 1.000; bootstrap: 95%)
  • ENSMODP00000023139 (score: 1.000; bootstrap: 83%)
  • ENSMODP00000020292 (score: 0.113)

From Inparanoid:20070104

Mus musculus

  • MGI:2146030 (score: 1.000; bootstrap: 86%)

From Inparanoid:20070104

Oryza gramene

  • Q7XQ00 (score: 0.458)
  • Q7XIZ0 (score: 1.000; bootstrap: 85%)
  • Q7XUE4 (score: 0.285)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000031789 (score: 1.000; bootstrap: 98%)
  • ENSPTRP00000023362 (score: 0.088)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000021153 (score: 1.000; bootstrap: 87%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YDR387C (score: 1.000; bootstrap: 98%)
  • YDR497C (score: 0.057)
  • YOL103W (score: 0.051)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPAC4F85 (score: 1.000; bootstrap: 95%)
  • SPAC20G83 (score: 0.297)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000135157 (score: 1.000; bootstrap: 51%)
  • NEWSINFRUP00000174671 (score: 1.000; bootstrap: 93%)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00031029001 (score: 1.000; bootstrap: 82%)
  • GSTENP00021878001 (score: 0.151)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000033917 (score: 1.000; bootstrap: 84%)
  • ENSXETP00000016911 (score: 0.177)
  • ENSXETP00000016929 (score: 0.093)

From Inparanoid:20070104

Shigella flexneri

GALP

From SHIGELLACYC

E. coli O157

GALP

From ECOO157CYC


Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Superfamily (EcoliWiki Page)

SUPERFAMILY:103473

Pfam (EcoliWiki Page)

PF00083 Sugar (and other) transporter

EcoCyc

EcoCyc:EG12148

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12148

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002048

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2068

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009654

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
  7. Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

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