galE:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
galE |
|---|---|
| Mnemonic |
Galactose |
| Synonyms |
ECK0748, b0759, JW0742, galD[1], galD |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
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MG1655 |
17.03 minutes |
MG1655: 791278..790262 |
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NC_012967: 772684..771668 |
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NC_012759: 693230..694246 |
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W3110 |
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W3110: 792477..791461 |
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DH10B: 845206..844190 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
790262 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
Strain DH10B |
S123F |
Nonsynonomous mutation |
This is a SNP in E. coli K-12 Strain DH10B | ||||
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ΔgalE (Keio:JW0742) |
deletion |
deletion |
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galE::Tn5KAN-2 (FB20233) |
Insertion at nt 824 in Minus orientation |
does not contain pKD46 | |||||
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galE45(GalS) |
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galE9(GalS) |
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galE28(GalS) |
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galE15(GalS) |
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galE54(GalS) |
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galE66(GalS) |
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galE9999(GalS) |
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galE537(GalS)::Tn10 |
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ΔgalE740::kan |
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0742 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAGAGTTCTGGTTACCGGTGG Primer 2:CCATCGGGATATCCCTGTGGATG | |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: 69% [11] | ||
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zbh-29::Tn10 |
Linked marker |
est. P1 cotransduction: 30% [11] | |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Schlesinger, DH et al. (1974) Localization of translation initiation in the message for E. coli UDP-galactose 4-epimerase: the amino terminal sequence. Biochem. Biophys. Res. Commun. 61 282-9 PubMed
- ↑ Ohba, A et al. (1997) Amounts of proteins altered by mutations in the dnaA gene of Escherichia coli. FEBS Lett. 404 125-8 PubMed
- ↑ Durfee, T et al. (2008) The complete genome sequence of Escherichia coli DH10B: insights into the biology of a laboratory workhorse. J. Bacteriol. 190 2597-606 PubMed
- ↑ 5.0 5.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 6.0 6.1 6.2 CGSC: The Coli Genetics Stock Center
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Kalckar, HM et al. (1959) HEREDITARY DEFECTS IN GALACTOSE METABOLISM IN ESCHERICHIA COLI MUTANTS, I. DETERMINATION OF ENZYME ACTIVITIES. Proc. Natl. Acad. Sci. U.S.A. 45 1776-86 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
