PMID:9119048
| Citation |
Ohba, A, Mizushima, T, Katayama, T and Sekimizu, K (1997) Amounts of proteins altered by mutations in the dnaA gene of Escherichia coli. FEBS Lett. 404:125-8 |
|---|---|
| Abstract |
We identified proteins whose amounts were altered in a temperature-sensitive dnaA46 mutant of Escherichia coli. Proteins whose amounts were increased in the mutant were serine hydroxymethyltransferase, beta-ketoacyl [acyl carrier protein] synthase II, long-chain fatty acid transport protein, and UDP-glucose 4-epimerase, while the decreased ones were flagellin and D-ribose-binding protein. Transformation of the mutant with a plasmid containing the wild type dnaA gene complemented the phenotype. As pulse-labeling experiments revealed that the rates of synthesis of the proteins were altered in the mutant, DnaA protein may be involved in expression of these proteins. |
| Links | |
| Keywords |
Amino Acid Sequence; Bacterial Outer Membrane Proteins/biosynthesis; Bacterial Proteins/biosynthesis; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Bacterial Proteins/isolation & purification; Carrier Proteins/biosynthesis; DNA Replication; DNA-Binding Proteins/biosynthesis; DNA-Binding Proteins/genetics; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Fatty Acid Transport Proteins; Flagellin/biosynthesis; Genetic Complementation Test; Glycine Hydroxymethyltransferase/biosynthesis; Molecular Sequence Data; Mutagenesis; Periplasmic Binding Proteins; Plasmids; Recombinant Proteins/biosynthesis; Ribose/metabolism; Temperature; Transformation, Bacterial; UDPglucose 4-Epimerase/biosynthesis |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
