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Standard Name	fpr
Gene Synonym(s)	ECK3916, b3924, JW3895, mvrA, flxR, G7964^[1]^[2]
Product Desc.	FLAVONADPREDUCT^[2]^[3]; Component of anaerobic nucleoside-triphosphate reductase activating system^[2]^[3] Ferredoxin NADP+ reductase; anaerobic; cloned fpr gene suppresses unlinked mvrA mutation^[4]
Product Synonyms(s)	ferredoxin-NADP reductase^[1], B3924^[2]^[1], FlxR^[2]^[1], MvrA^[2]^[1], Fpr^[2]^[1] , ECK3916, flxR, JW3895, b3924
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): fpr^[2], OP00261
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	fpr
Mnemonic	Ferredoxin NADP+ reductase
Synonyms	ECK3916, b3924, JW3895, mvrA, flxR, G7964^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	88.62 minutes	MG1655: 4112495..4111749 <gbrowseImage> name=NC_000913:4111749..4112495 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4093954..4093208 <gbrowseImage> name=NC_012967:4093208..4093954 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4001418..4002164 <gbrowseImage> name=NC_012759:4001418..4002164 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3522209..3522955 <gbrowseImage> name=NC_007779:3522209..3522955 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4212192..4211446 <gbrowseImage> name=NC_010473:4211446..4212192 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	4111752	Edman degradation	PMID:7642583 PMID:7961651 PMID:8449868
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
Δfpr (Keio:JW3895)	deletion	deletion	PMID:16738554	Shigen CGSC10810^[5]
Δfpr-783::kan			PMID:16738554	CGSC:100988
Strain BW2952	G126A		PMID:19376874		Relative to MG1655.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3895	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCGCTGATTGGGTAACAGGCAA Primer 2:CCCCAGTAATGCTCCGCTGTCAT
4H12	Kohara Phage	Genobase	PMID:3038334
8B10	Kohara Phage	Genobase	PMID:3038334
zih-35::Tn10	Linked marker	CAG18495 = CGSC7467^[5]	est. P1 cotransduction: 12% ^[6]
pflD501::Tn10	Linked marker	CAG18477 = CGSC7470^[5]	est. P1 cotransduction: 29% ^[6] Synonyms:zij-501::Tn10 nnnCAG18477 also carries metF79(Am) (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10628	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10628	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000621	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:948414	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1480	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012818	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Fpr
Synonyms	ferredoxin-NADP reductase^[1], B3924^[2]^[1], FlxR^[2]^[1], MvrA^[2]^[1], Fpr^[2]^[1] , ECK3916, flxR, JW3895, b3924
Product description	FLAVONADPREDUCT^[2]^[3]; Component of anaerobic nucleoside-triphosphate reductase activating system^[2]^[3] Ferredoxin NADP+ reductase; anaerobic; cloned fpr gene suppresses unlinked mvrA mutation^[4]
EC number (for enzymes)	1.18.1.2^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0042493	response to drug	PMID:2834327	IMP: Inferred from Mutant Phenotype		P		complete
	GO:0004324	ferredoxin-NADP+ reductase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:1.18.1.2	F	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001433	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR008333	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017927	P	Seeded from EcoCyc (v14.0)	complete
	GO:0055114	oxidation reduction	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0560	P	Seeded from EcoCyc (v14.0)	complete
	GO:0004324	ferredoxin-NADP+ reductase activity	PMID:7042345	IDA: Inferred from Direct Assay		F	from EcoCyc_v23.0	complete
	GO:0004324	ferredoxin-NADP+ reductase activity	PMID:8449868	IDA: Inferred from Direct Assay		F	from EcoCyc_v23.0	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of anaerobic nucleoside-triphosphate reductase activating system	could be indirect
Protein	deoC		PMID:16606699	Experiment(s):EBI-1147065
Protein	rpsD		PMID:16606699	Experiment(s):EBI-1147065
Protein	nadE		PMID:16606699	Experiment(s):EBI-1147065
Protein	blc		PMID:16606699	Experiment(s):EBI-1147065
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MADWVTGKVT KVQNWTDALF SLTVHAPVLP FTAGQFTKLG LEIDGERVQR AYSYVNSPDN PDLEFYLVTV PDGKLSPRLA ALKPGDEVQV VSEAAGFFVL DEVPHCETLW MLATGTAIGP YLSILQLGKD LDRFKNLVLV HAARYAADLS YLPLMQELEK RYEGKLRIQT VVSRETAAGS LTGRIPALIE SGELESTIGL PMNKETSHVM LCGNPQMVRD TQQLLKETRQ MTKHLRRRPG HMTAEHYW
Length	248
Mol. Wt	27.75 kDa
pI	6.6 (calculated)
Extinction coefficient	33,920 - 34,170 (calc based on 8 Y, 4 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P28861
Domain	6..98	PF00970 Oxidoreductase FAD-binding domain		PMID:19920124
Domain	111..223	PF00175 Oxidoreductase NAD-binding domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fpr taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131762	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948414	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012818	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P28861	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10628	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10628	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948414	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000621	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1480	Escherichia coli str. K-12 substr. MG1655
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Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Reference(s)
Protein	E. coli K-12 MC4100	2.12E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI	PMID:18304323
Protein	E. coli K-12 MG1655	674	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	495	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
Protein	E. coli K-12 MG1655	688	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
fpr
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4112475..4112515 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3924 (EcoliWiki Page)	NCBI GEO profiles for fpr
microarray	GenExpDB:b3924 (EcoliWiki Page)	Summary of data for fpr from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4111975..4112174) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ04; Well:C8^[7]
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Notes

Accessions Related to fpr Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10628	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1480	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3924
EcoGene	EcoGene:EG10628	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000621	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012818	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Oryza gramene	Q5VR12 (score: 1.000; bootstrap: 100%) Q9ZPN0 (score: 0.298) Q5N760 (score: 0.298) Q8W2K4 (score: 0.280) Q6AVN1 (score: 0.280) Q6ZC37 (score: 0.197) Q6ZC33 (score: 0.197) P16081 (score: 0.197) P27967 (score: 0.187) Q6ZHH7 (score: 0.185) P27968 (score: 0.181) P27969 (score: 0.181) Q9XGW5 (score: 0.181) P17571 (score: 0.181) P49102 (score: 0.176) Q43265 (score: 0.169) P39871 (score: 0.113)	From Inparanoid:20070104
Xenopus tropicalis	ENSXETP00000021892 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	FPR	From SHIGELLACYC
E. coli O157	FPR	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00175 Oxidoreductase NAD-binding domain
Pfam (EcoliWiki Page)	PF00970 Oxidoreductase FAD-binding domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:52343
Panther (EcoliWiki Page)	PTHR19384:SF51
Superfamily (EcoliWiki Page)	SUPERFAMILY:63380
EcoCyc	EcoCyc:EG10628	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10628	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000621	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1480	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0012818	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 ^1.13 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

fpr:On One Page

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Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to fpr Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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