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Gene |

Product(s) |

Expression|

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Standard Name	fmt
Gene Synonym(s)	ECK3274, b3288, JW3249, yhdD, ftm^[1]
Product Desc.	10-formyltetrahydrofolate:L-methionyl-tRNA(fMet) N-formyltransferase^[2]^[3] Methionyl-tRNA formyltransferase^[4]
Product Synonyms(s)	10-formyltetrahydrofolate:L-methionyl-tRNA(fMet) N-formyltransferase^[1], B3288^[2]^[1], Ftm^[2]^[1], YhdD^[2]^[1], Fmt^[2]^[1] , ECK3274, JW3249, yhdD, b3288
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): def-fmt^[2]
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	fmt
Mnemonic	fMet-tRNA formyltransferase
Synonyms	ECK3274, b3288, JW3249, yhdD, ftm^[1]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	73.98 minutes	MG1655: 3432236..3433183 <gbrowseImage> name=NC_000913:3432236..3433183 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 3362119..3363066 <gbrowseImage> name=NC_012967:3362119..3363066 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 3319393..3320340 <gbrowseImage> name=NC_012759:3319393..3320340 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4206202..4205255 <gbrowseImage> name=NC_007779:4205255..4206202 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3529981..3530928 <gbrowseImage> name=NC_010473:3529981..3530928 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3432239	Edman degradation	PMID:1624424
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW3249	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCTCAGAATCACTACGTATTAT Primer 2:CCGACCAGACGGTTGCCCGGAAC
smg-3082::Tn10	Linked marker	CAG12071 = CGSC7444^[5]	est. P1 cotransduction: 89% ^[6] Synonyms:zhb-3082::Tn10, zhd-3082::Tn10
zhf-3084::Tn10	Linked marker	CAG18456 = CGSC7576^[5]	est. P1 cotransduction: 1% ^[6] Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11268	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11268	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001242	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947779	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1247	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010781	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	Fmt
Synonyms	10-formyltetrahydrofolate:L-methionyl-tRNA(fMet) N-formyltransferase^[1], B3288^[2]^[1], Ftm^[2]^[1], YhdD^[2]^[1], Fmt^[2]^[1] , ECK3274, JW3249, yhdD, b3288
Product description	10-formyltetrahydrofolate:L-methionyl-tRNA(fMet) N-formyltransferase^[2]^[3] Methionyl-tRNA formyltransferase^[4]
EC number (for enzymes)	2.1.2.9^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004479	methionyl-tRNA formyltransferase activity	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00182	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004479	methionyl-tRNA formyltransferase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005794	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004479	methionyl-tRNA formyltransferase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:2.1.2.9	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004479	methionyl-tRNA formyltransferase activity	PMID:6989606	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0006412	translation	GOA:hamap GO_REF:0000020	IEA: Inferred from Electronic Annotation	HAMAP:MF_00182	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006412	translation	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005794	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006412	translation	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0648	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009058	biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002376	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009058	biosynthetic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR005793	P	Seeded from EcoCyc (v14.0)	complete
	GO:0019988	charged-tRNA amino acid modification	PMID:6989606	IDA: Inferred from Direct Assay		P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	rfaP	PMID:16606699	Experiment(s):EBI-1145369
Protein	rpsG	PMID:16606699	Experiment(s):EBI-1145369
Protein	rplQ	PMID:16606699	Experiment(s):EBI-1145369
Protein	rplA	PMID:16606699	Experiment(s):EBI-1145369
Protein	rpsM	PMID:16606699	Experiment(s):EBI-1145369
Protein	ydfK	PMID:16606699	Experiment(s):EBI-1145369
Protein	rplD	PMID:16606699	Experiment(s):EBI-1145369
Protein	rpsI	PMID:16606699	Experiment(s):EBI-1145369
Protein	rpsD	PMID:16606699	Experiment(s):EBI-1145369, EBI-1145434
Protein	ynaE	PMID:16606699	Experiment(s):EBI-1145369
Protein	rplI	PMID:16606699	Experiment(s):EBI-1145369
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
cytoplasm			From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MSESLRIIFA GTPDFAARHL DALLSSGHNV VGVFTQPDRP AGRGKKLMPS PVKVLAEEKG LPVFQPVSLR PQENQQLVAE LQADVMVVVA YGLILPKAVL EMPRLGCINV HGSLLPRWRG AAPIQRSLWA GDAETGVTIM QMDVGLDTGD MLYKLSCPIT AEDTSGTLYD KLAELGPQGL ITTLKQLADG TAKPEVQDET LVTYAEKLSK EEARIDWSLS AAQLERCIRA FNPWPMSWLE IEGQPVKVWK ASVIDTATNA APGTILEANK QGIQVATGDG ILNLLSLQPA GKKAMSAQDL LNSRREWFVP GNRLV
Length	315
Mol. Wt	34.168 kDa
pI	5.5 (calculated)
Extinction coefficient	44,460 - 44,835 (calc based on 4 Y, 7 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed	UniProt:P23882
Domain	207..305	PF02911 Formyl transferase, C-terminal domain		PMID:19920124
Domain	5..184	PF00551 Formyl transferase		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fmt taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131167	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947779	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010781	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P23882	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11268	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11268	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947779	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001242	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1247	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.04E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	25.687+/-0.218	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.102956705	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	2766	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	435	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1010	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
def-fmt
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Figure courtesy of RegulonDB

</protect>

Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3432216..3432256 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

</protect>

Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

</protect>

Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3288 (EcoliWiki Page)	NCBI GEO profiles for fmt
microarray	GenExpDB:b3288 (EcoliWiki Page)	Summary of data for fmt from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to fmt Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11268	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1247	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3288
EcoGene	EcoGene:EG11268	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001242	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010781	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Anopheles gambiae	ENSANGP00000022001 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Arabidopsis thaliana	AT1G66520 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Drosophila melanogaster	FBgn0039836 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Oryza gramene	Q5N7L5 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Pan troglodytes	ENSPTRP00000009141 (score: 1.000; bootstrap: 75%) ENSPTRP00000026424 (score: 0.640)	From Inparanoid:20070104
Saccharomyces cerevisiae	YBL013W (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Schizosaccharomyces pombe	SPAC18059c (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Shigella flexneri	FMT	From SHIGELLACYC
E. coli O157	FMT	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF02911 Formyl transferase, C-terminal domain
Pfam (EcoliWiki Page)	PF00551 Formyl transferase
Superfamily (EcoliWiki Page)	SUPERFAMILY:50486
Superfamily (EcoliWiki Page)	SUPERFAMILY:53328
Panther (EcoliWiki Page)	PTHR11138:SF0
EcoCyc	EcoCyc:EG11268	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11268	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001242	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1247	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0010781	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 ^1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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Gene

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Genetic Resources

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Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to fmt Expression

Notes

Links

Evolution

Homologs in Other Organisms

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Notes

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