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On One Page

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Gene |

Product(s) |

Expression|

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Standard Name	flgL
Gene Synonym(s)	ECK1068, b1083, JW1070, flaT, flaU^[1]^[2]
Product Desc.	flagellar biosynthesis; hook-filament junction protein^[2]^[3]; Component of Flagellum^[3] Flagellar synthesis, hook-associated protein^[4]
Product Synonyms(s)	flagellar hook-filament junction protein^[1], B1083^[2]^[1], FlaU^[2]^[1], FlaT^[2]^[1], FlgL^[2]^[1] , ECK1068, flaT, JW1070, b1083
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): flgK^[2]
Regulation/Activity
Quick Links
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Notes

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Overexpression causes mucoidy, decreases the amount of biofilm formed, and prevents flagella formation. Flagellar regulon.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	flgL
Mnemonic	Flagella
Synonyms	ECK1068, b1083, JW1070, flaT, flaU^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	24.55 minutes	MG1655: 1139256..1140209 <gbrowseImage> name=NC_000913:1139256..1140209 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1154602..1155555 <gbrowseImage> name=NC_012967:1154602..1155555 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1043181..1044134 <gbrowseImage> name=NC_012759:1043181..1044134 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1141610..1142563 <gbrowseImage> name=NC_007779:1141610..1142563 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1194701..1195654 <gbrowseImage> name=NC_010473:1194701..1195654 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Description	Reference	Availability	Comments
ΔflgL (Keio:JW1070)	deletion	deletion		PMID:16738554	Shigen
flgL::Tn5KAN-2 (FB20099)	Insertion at nt 555 in Plus orientation			PMID:15262929	E. coli Genome Project:FB20099	does not contain pKD46
flgLR168C		R168C	(in HAP3; exhibits impaired swimming only when in contact with a solid surface or a semisolid matrix)			Strain variation; seeded from UniProt:P29744
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1070	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCGTTTCAGTACACAGATGAT Primer 2:CCTTTGCTGAGCTGGAAGAGCGA
E3G11	Kohara Phage	Genobase	PMID:3038334
14C1	Kohara Phage	Genobase	PMID:3038334
zcc-282::Tn10	Linked marker	CAG18466 = CGSC7359^[5]	est. P1 cotransduction: 1% ^[6]
zce-726::Tn10	Linked marker	CAG12078 = CGSC7361^[5]	est. P1 cotransduction: 57% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11545	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11545	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001499	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945646	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1507	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003666	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FlgL
Synonyms	flagellar hook-filament junction protein^[1], B1083^[2]^[1], FlaU^[2]^[1], FlaT^[2]^[1], FlgL^[2]^[1] , ECK1068, flaT, JW1070, b1083
Product description	flagellar biosynthesis; hook-filament junction protein^[2]^[3]; Component of Flagellum^[3] Flagellar synthesis, hook-associated protein^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0001539	ciliary or flagellar motility	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001492	P	Seeded from EcoCyc (v14.0)	complete
	GO:0001539	ciliary or flagellar motility	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013384	P	Seeded from EcoCyc (v14.0)	complete
	GO:0005198	structural molecule activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001492	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005576	extracellular region	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0964	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005576	extracellular region	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0243	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009288	bacterial-type flagellum	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0975	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009288	bacterial-type flagellum	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0307	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009420	bacterial-type flagellum filament	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001492	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009424	bacterial-type flagellum hook	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR013384	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of Flagellum	could be indirect
Protein	uvrY		PMID:16606699	Experiment(s):EBI-1138794
Protein	phoB		PMID:16606699	Experiment(s):EBI-1138794
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MRFSTQMMYQ QNMRGITNSQ AEWMKYGEQM STGKRVVNPS DDPIAASQAV VLSQAQAQNS QYTLARTFAT QKVSLEESVL SQVTTAIQNA QEKIVYASNG TLSDDDRASL ATDIQGLRDQ LLNLANTTDG NGRYIFAGYK TETAPFSEEK GKYVGGAESI KQQVDASRSM VIGHTGDKIF DSITSNAVAE PDGSASETNL FAMLDSAIAA LKTPVADSEA DKETAAAALD KTNRGLKNSL NNVLTVRAEL GTQLNELESL DSLGSDRALG QTQQMSDLVD VDWNATISSY IMQQTALQAS YKAFTDMQGL SLFQLSK
Length	317
Mol. Wt	34.281 kDa
pI	4.5 (calculated)
Extinction coefficient	24,410 (calc based on 9 Y, 2 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	3..141	PF00669 Bacterial flagellin N-terminal helical region		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=flgL taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129046	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945646	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003666	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P29744	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11545	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11545	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945646	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001499	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1507	Escherichia coli str. K-12 substr. MG1655
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Notes

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	39	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	39	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	11^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
flgK
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1139236..1139276 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1083 (EcoliWiki Page)	NCBI GEO profiles for flgL
microarray	GenExpDB:b1083 (EcoliWiki Page)	Summary of data for flgL from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to flgL Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11545	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1507	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1083
EcoGene	EcoGene:EG11545	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001499	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003666	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00669 Bacterial flagellin N-terminal helical region
Superfamily (EcoliWiki Page)	SUPERFAMILY:64518
EcoCyc	EcoCyc:EG11545	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11545	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001499	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1507	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003666	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

flgL:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to flgL Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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