flgC:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

Quickview

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Standard Name

flgC

Gene Synonym(s)

ECK1059, b1074, JW1061, flaW, fla FIII, fla, F, FIII[1][2]

Product Desc.

flagellar basal-body rod protein FlgC[2][3];

Component of Flagellar Motor Complex[2][3]; Flagellum[3]

Flagellar basal body rod subunit[4]

Product Synonyms(s)

flagellar component of cell-proximal portion of basal-body rod[1], B1074[2][1], Fla[2][1], FlaW[2][1], FlgC[2][1] , ECK1059, flaW, JW1061, b1074

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): flgBCDEFGHIJ[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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Flagellar regulon.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

flgC

Mnemonic

Flagella

Synonyms

ECK1059, b1074, JW1061, flaW, fla FIII, fla, F, FIII[1][2]

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

24.37 minutes 

MG1655: 1130661..1131065
<gbrowseImage> name=NC_000913:1130661..1131065 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1146010..1146414
<gbrowseImage> name=NC_012967:1146010..1146414 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1034586..1034990
<gbrowseImage> name=NC_012759:1034586..1034990 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1133015..1133419
<gbrowseImage> name=NC_007779:1133015..1133419 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1186106..1186510
<gbrowseImage> name=NC_010473:1186106..1186510 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔflgC (Keio:JW1061)

deletion

deletion

PMID:16738554

Shigen
CGSC9018[5]

flgC::Tn5KAN-2 (FB20096)

Insertion at nt 96 in Minus orientation

PMID:15262929

E. coli Genome Project:FB20096

does not contain pKD46

ΔflgC743::kan

PMID:16738554

CGSC:100902


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1061

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCACTGCTGAATATTTTTGA

Primer 2:CCTTGACCGAGCGTAAGGGTTTT

E4H10S

Kohara Phage

Genobase

PMID:3038334

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[5]

est. P1 cotransduction: 2% [6]

zce-726::Tn10

Linked marker

CAG12078 = CGSC7361[5]

est. P1 cotransduction: 40% [6]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G359

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG14267

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002680

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946687

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4015

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003643

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

FlgC

Synonyms

flagellar component of cell-proximal portion of basal-body rod[1], B1074[2][1], Fla[2][1], FlaW[2][1], FlgC[2][1] , ECK1059, flaW, JW1061, b1074

Product description

flagellar basal-body rod protein FlgC[2][3];

Component of Flagellar Motor Complex[2][3]; Flagellum[3]

Flagellar basal body rod subunit[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0001539

ciliary or flagellar motility

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001444

P

Seeded from EcoCyc (v14.0)

complete

GO:0001539

ciliary or flagellar motility

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019776

P

Seeded from EcoCyc (v14.0)

complete

GO:0003774

motor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001444

F

Seeded from EcoCyc (v14.0)

complete

GO:0003774

motor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019776

F

Seeded from EcoCyc (v14.0)

complete

GO:0005198

structural molecule activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001444

F

Seeded from EcoCyc (v14.0)

complete

GO:0005198

structural molecule activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019776

F

Seeded from EcoCyc (v14.0)

complete

GO:0009288

bacterial-type flagellum

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001444

C

Seeded from EcoCyc (v14.0)

complete

GO:0009288

bacterial-type flagellum

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019776

C

Seeded from EcoCyc (v14.0)

complete

GO:0009288

bacterial-type flagellum

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0975

C

Seeded from EcoCyc (v14.0)

complete

GO:0009425

bacterial-type flagellum basal body

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0142

C

Seeded from EcoCyc (v14.0)

complete

GO:0019861

flagellum

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006299

C

Seeded from EcoCyc (v14.0)

complete

GO:0019861

flagellum

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010930

C

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of Flagellar Motor Complex

could be indirect

Protein

Subunits of Flagellum

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Periplasm

PMID:12730325

EchoLocation:flgC


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MALLNIFDIA GSALTAQSQR LNVAASNLAN ADSVTGPDGQ PYRAKQVVFQ VNAAPGAATG
GVKVADVIES QAPDKLVYEP GNPLADAKGY VKMPNVDVVG EMVNTMSASR SYQANVEVLN
TVKSMMLKTL TLGQ
Length

134

Mol. Wt

13.967 kDa

pI

5.1 (calculated)

Extinction coefficient

5,960 (calc based on 4 Y, 0 W, and 0 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

7..38

PF00460 Flagella basal body rod protein

PMID:19920124

Domain

94..132

PF06429 Domain of unknown function (DUF1078)

PMID:19920124

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=flgC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129037

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946687

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003643

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ABX2

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G359

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG14267

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946687

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002680

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4015

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MG1655

83

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

17

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

0a

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

Low confidence in the sequencing data set.

PMID: 24766808

Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

flgBCDEFGHIJ

Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1130641..1130681 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference


Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b1074 (EcoliWiki Page)

NCBI GEO profiles for flgC

microarray

GenExpDB:b1074 (EcoliWiki Page)

Summary of data for flgC from multiple microarray studies


Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes

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Notes

Accessions Related to flgC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:G359

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4015

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1074

EcoGene

EcoGene:EG14267

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002680

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003643

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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<protect> See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF00460 Flagella basal body rod protein

Pfam (EcoliWiki Page)

PF06429 Domain of unknown function (DUF1078)

EcoCyc

EcoCyc:G359

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG14267

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002680

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB4015

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003643

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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