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Expression|

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Standard Name	flgB
Gene Synonym(s)	ECK1058, b1073, JW1060, flbA, fla FII, fla, F, fii^[1]^[2]
Product Desc.	flagellar basal-body rod protein FlgB^[2]^[3]; Component of Flagellar Motor Complex^[2]^[3]; Flagellum^[3] Flagellar basal body rod subunit^[4]
Product Synonyms(s)	flagellar component of cell-proximal portion of basal-body rod^[1], B1073^[2]^[1], Fla^[2]^[1], FlbA^[2]^[1], FlgB^[2]^[1] , ECK1058, flbA, JW1060, b1073
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): flgBCDEFGHIJ^[2]
Regulation/Activity
Quick Links
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</protect> See Help:Quickview for help with entering information in the Quickview table. <protect></protect>

Notes

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Flagellar regulon.^[4]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	flgB
Mnemonic	Flagella
Synonyms	ECK1058, b1073, JW1060, flbA, fla FII, fla, F, fii^[1]^[2]
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	24.36 minutes	MG1655: 1130241..1130657 <gbrowseImage> name=NC_000913:1130241..1130657 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 1145590..1146006 <gbrowseImage> name=NC_012967:1145590..1146006 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 1034166..1034582 <gbrowseImage> name=NC_012759:1034166..1034582 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 1132595..1133011 <gbrowseImage> name=NC_007779:1132595..1133011 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 1185686..1186102 <gbrowseImage> name=NC_010473:1185686..1186102 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability	Comments
flgB(del) (Keio:JW1060)	deletion	deletion	PMID:16738554	Shigen CGSC9017^[5]
flgB::Tn5KAN-2 (FB20095)	Insertion at nt 261 in Plus orientation		PMID:15262929	E. coli Genome Project:FB20095	does not contain pKD46
flgB742(del)::kan			PMID:16738554	CGSC:100900
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW1060	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCCTCGATAAGCTCGACGCCGC Primer 2:CCATTTCCGCTCTGTAAAACGTT
E4H10S	Kohara Phage	Genobase	PMID:3038334
zcc-282::Tn10	Linked marker	CAG18466 = CGSC7359^[5]	est. P1 cotransduction: 2% ^[6]
zce-726::Tn10	Linked marker	CAG12078 = CGSC7361^[5]	est. P1 cotransduction: 39% ^[6]
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G358	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14266	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002679	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:945678	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4014	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003641	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FlgB
Synonyms	flagellar component of cell-proximal portion of basal-body rod^[1], B1073^[2]^[1], Fla^[2]^[1], FlbA^[2]^[1], FlgB^[2]^[1] , ECK1058, flbA, JW1060, b1073
Product description	flagellar basal-body rod protein FlgB^[2]^[3]; Component of Flagellar Motor Complex^[2]^[3]; Flagellum^[3] Flagellar basal body rod subunit^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0001539	ciliary or flagellar motility	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001444	P	Seeded from EcoCyc (v14.0)	complete
	GO:0001539	ciliary or flagellar motility	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR019776	P	Seeded from EcoCyc (v14.0)	complete
	GO:0003774	motor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001444	F	Seeded from EcoCyc (v14.0)	complete
	GO:0003774	motor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR019776	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005198	structural molecule activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001444	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005198	structural molecule activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR019776	F	Seeded from EcoCyc (v14.0)	complete
	GO:0009288	bacterial-type flagellum	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001444	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009288	bacterial-type flagellum	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR019776	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009288	bacterial-type flagellum	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0975	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009425	bacterial-type flagellum basal body	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0142	C	Seeded from EcoCyc (v14.0)	complete
	GO:0019861	flagellum	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR006300	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of Flagellar Motor Complex	could be indirect
Protein	dps		PMID:16606699	Experiment(s):EBI-1138768
Protein	rfaG		PMID:16606699	Experiment(s):EBI-1138768
Protein	ybhS		PMID:16606699	Experiment(s):EBI-1138768
Protein	nadE		PMID:16606699	Experiment(s):EBI-1138768
Protein	rplB		PMID:16606699	Experiment(s):EBI-1138768
Protein	Subunits of Flagellum	could be indirect
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
Periplasm		PMID:12730325	EchoLocation:flgB
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MLDKLDAALR FQQEALNLRA QRQEVLAANI ANADTPGYQA RDIDFASELK KVMQRGRDAT SVVALTMTST QHIPAQALTP PTAELQYRIP DQPSLDGNTV DMDRERTQFA DNSLQYQMSL SALSGQIKGM MNVLQSGN
Length	138
Mol. Wt	15.24 kDa
pI	4.9 (calculated)
Extinction coefficient	4,470 (calc based on 3 Y, 0 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Initiator Methionine	1	Removed (By similarity)	UniProt:P0ABW9
Domain	9..39	PF00460 Flagella basal body rod protein		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=flgB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16129036	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:945678	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003641	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0ABW9	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:G358	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14266	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:945678	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002679	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4014	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	78	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	21	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	8^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
flgBCDEFGHIJ
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:1130221..1130261 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b1073 (EcoliWiki Page)	NCBI GEO profiles for flgB
microarray	GenExpDB:b1073 (EcoliWiki Page)	Summary of data for flgB from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (1129999..1130302) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ20; Well:B8^[7]
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Notes

Accessions Related to flgB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:G358	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4014	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b1073
EcoGene	EcoGene:EG14266	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002679	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003641	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	FLGB	From SHIGELLACYC
E. coli O157	FLGB	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00460 Flagella basal body rod protein
EcoCyc	EcoCyc:G358	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG14266	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002679	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB4014	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0003641	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 ^1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.00 ^2.01 ^2.02 ^2.03 ^2.04 ^2.05 ^2.06 ^2.07 ^2.08 ^2.09 ^2.10 ^2.11 ^2.12 ^2.13 ^2.14 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 ^3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 ^4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

flgB:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to flgB Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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