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On One Page

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Gene |

Product(s) |

Expression|

Evolution |

References |

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Standard Name	fecA
Gene Synonym(s)	ECK4281, b4291, JW4251^[1], JW4251
Product Desc.	outer membrane receptor; citrate-dependent iron transport, outer membrane receptor^[2]^[3]; Component of FecAR Signal Transduction System^[2]^[3] Outer membrane ferric citrate receptor; ferric citrate uptake, TonB-dependent; signals FecR to activate FecI^[4]
Product Synonyms(s)	KpLE2 phage-like element; ferric citrate outer membrane transporter^[1], B4291^[2]^[1], FecA^[2]^[1] , ECK4281, JW4251, b4291
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): fecABCDE^[2], OP00206, fecIRABCDE
Regulation/Activity
Quick Links
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Notes

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Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	fecA
Mnemonic	Ferric citrate
Synonyms	ECK4281, b4291, JW4251^[1], JW4251
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	97.26 minutes, 97.26 minutes	MG1655: 4514700..4512376 <gbrowseImage> name=NC_000913:4512376..4514700 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 4501709..4499385 <gbrowseImage> name=NC_012967:4499385..4501709 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 4451111..4453435 <gbrowseImage> name=NC_012759:4451111..4453435 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 4521357..4519033 <gbrowseImage> name=NC_007779:4519033..4521357 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 4616508..4614184 <gbrowseImage> name=NC_010473:4614184..4616508 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	4512379	Edman degradation	PMID:2836368
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Reference	Availability
ΔfecA (Keio:JW4251)	deletion	deletion	PMID:16738554	Shigen CGSC11049^[5]
ΔfecA758::kan			PMID:16738554	CGSC:100818
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW4251	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCACGCCGTTACGCGTTTTTCG Primer 2:CCGAACTTCAACGACCCCTGCAT
16H7S	Kohara Phage	Genobase	PMID:3038334
7G7	Kohara Phage	Genobase	PMID:3038334
9H9	Kohara Phage	Genobase	PMID:3038334
zjf-920::Tn10	Linked marker	CAG12019 = CGSC7484^[5]	est. P1 cotransduction: 2% ^[6] Synonyms:zjh-901::Tn10, zjg-920::Tn10
mdoB202::Tn10	Linked marker	CAG18430 = CGSC7488^[5]	est. P1 cotransduction: % ^[6] Synonyms:zjj-202::Tn10, zji-202::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10286	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10286	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000280	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:946427	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0282	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014067	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	FecA
Synonyms	KpLE2 phage-like element; ferric citrate outer membrane transporter^[1], B4291^[2]^[1], FecA^[2]^[1] , ECK4281, JW4251, b4291
Product description	outer membrane receptor; citrate-dependent iron transport, outer membrane receptor^[2]^[3]; Component of FecAR Signal Transduction System^[2]^[3] Outer membrane ferric citrate receptor; ferric citrate uptake, TonB-dependent; signals FecR to activate FecI^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004872	receptor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000531	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004872	receptor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010105	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004872	receptor activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012910	F	Seeded from EcoCyc (v14.0)	complete
	GO:0004872	receptor activity	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0675	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005215	transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000531	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005215	transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012910	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005506	iron ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010105	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005506	iron ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0408	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000531	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012910	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006810	transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006811	ion transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0406	P	Seeded from EcoCyc (v14.0)	complete
	GO:0006826	iron ion transport	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0410	P	Seeded from EcoCyc (v14.0)	complete
	GO:0009279	cell outer membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0998	C	Seeded from EcoCyc (v14.0)	complete
	GO:0009279	cell outer membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0040	C	Seeded from EcoCyc (v14.0)	complete
	GO:0015343	siderophore-iron transmembrane transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010105	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015891	siderophore transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR010105	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR000531	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR012910	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0019867	outer membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR011662	C	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of FecAR Signal Transduction System	could be indirect
Protein	rpoC		PMID:16606699	Experiment(s):EBI-1147810
Protein	putA		PMID:16606699	Experiment(s):EBI-1147810
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
Outer Membrane	PMID:2836368	EchoLocation:fecA
outer membrane		From EcoCyc^[3]
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MTPLRVFRKT TPLVNTIRLS LLPLAGLSFS AFAAQVNIAP GSLDKALNQY AAHSGFTLSV DASLTRGKQS NGLHGDYDVE SGLQQLLDGS GLQVKPLGNN SWTLEPAPAP KEDALTVVGD WLGDARENDV FEHAGARDVI RREDFAKTGA TTMREVLNRI PGVSAPENNG TGSHDLAMNF GIRGLNPRLA SRSTVLMDGI PVPFAPYGQP QLSLAPVSLG NMDAIDVVRG GGAVRYGPQS VGGVVNFVTR AIPQDFGIEA GVEGQLSPTS SQNNPKETHN LMVGGTADNG FGTALLYSGT RGSDWREHSA TRIDDLMLKS KYAPDEVHTF NSLLQYYDGE ADMPGGLSRA DYDADRWQST RPYDRFWGRR KLASLGYQFQ PDSQHKFNIQ GFYTQTLRSG YLEQGKRITL SPRNYWVRGI EPRYSQIFMI GPSAHEVGVG YRYLNESTHE MRYYTATSSG QLPSGSSPYD RDTRSGTEAH AWYLDDKIDI GNWTITPGMR FEHIESYQNN AITGTHEEVS YNAPLPALNV LYHLTDSWNL YANTEGSFGT VQYSQIGKAV QSGNVEPEKA RTWELGTRYD DGALTAEMGL FLINFNNQYD SNQTNDTVTA RGKTRHTGLE TQARYDLGTL TPTLDNVSIY ASYAYVNAEI REKGDTYGNL VPFSPKHKGT LGVDYKPGNW TFNLNSDFQS SQFADNANTV KESADGSTGR IPGFMLWGAR VAYDFGPQMA DLNLAFGVKN IFDQDYFIRS YDDNNKGIYA GQPRTLYMQG SLKF
Length	774
Mol. Wt	85.323 kDa
pI	5.9 (calculated)
Extinction coefficient	124,110 (calc based on 39 Y, 12 W, and 0 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-33	UniProt Manual:Signal Peptides	UniProt:P13036
Domain	525..773	PF00593 TonB dependent receptor		PMID:19920124
Domain	56..107	PF07660 Secretin and TonB N terminus short domain		PMID:19920124
Domain	129..244	PF07715 TonB-dependent Receptor Plug Domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=fecA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16132112	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:946427	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014067	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P13036	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG10286	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10286	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:946427	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000280	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0282	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	3.58E+02	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	E. coli K-12 MG1655	51	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	12	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	6^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
fecABCDE
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:4514680..4514720 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b4291 (EcoliWiki Page)	NCBI GEO profiles for fecA
microarray	GenExpDB:b4291 (EcoliWiki Page)	Summary of data for fecA from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
	GFP Fusion	Intergenic region (4514145..4514404) fused to gfpmut2.	OpenBioSystems	GFP fusion described in Zaslaver, et al. Plate:109-AZ17; Well:A3^[7]
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Notes

Accessions Related to fecA Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG10286	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0282	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b4291
EcoGene	EcoGene:EG10286	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000280	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014067	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Xenopus tropicalis	ENSXETP00000014184 (score: 1.000; bootstrap: 81%)	From Inparanoid:20070104
E. coli O157	Z4386	From ECOO157CYC
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Superfamily (EcoliWiki Page)	SUPERFAMILY:56935
Pfam (EcoliWiki Page)	PF00593 TonB dependent receptor
Pfam (EcoliWiki Page)	PF07660 Secretin and TonB N terminus short domain
Pfam (EcoliWiki Page)	PF07715 TonB-dependent Receptor Plug Domain
EcoCyc	EcoCyc:EG10286	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG10286	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120000280	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB0282	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0014067	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 ^3.3 ^3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^4.0 ^4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 CGSC: The Coli Genetics Stock Center
↑ ^6.0 ^6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ Zaslaver, A et al. (2006) A comprehensive library of fluorescent transcriptional reporters for Escherichia coli. Nat. Methods 3 623-8 PubMed

fecA:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to fecA Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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