entA:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

entA

Mnemonic

Enterochelin

Synonyms

ECK0589, b0596, JW0588[1], JW0588

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

13.53 minutes 

MG1655: 627774..628520
<gbrowseImage> name=NC_000913:627774..628520 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 611259..612005
<gbrowseImage> name=NC_012967:611259..612005 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 530534..531280
<gbrowseImage> name=NC_012759:530534..531280 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 627774..628520
<gbrowseImage> name=NC_007779:627774..628520 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 567106..567852
<gbrowseImage> name=NC_010473:567106..567852 source=DH10B preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 680366..681112
<gbrowseImage> name=NC_010473:680366..681112 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

627774

Edman degradation

PMID:2521622[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔentA (Keio:JW0588)

deletion

deletion

PMID:16738554[3]

Shigen

entA::Tn5KAN-2 (FB20211)

Insertion at nt 281 in Minus orientation

PMID:15262929[4]

E. coli Genome Project:FB20211

does not contain pKD46

entA403

PMID:2536681[5]

CGSC:4931

ΔentA

deletion

deletion

Auxotrophies

Deletion causes uptake of iron via an essential component of Enterobactin (also known as Enterochelin) iron transport system to decrease-Table 3.

PMID:4919746[6]

ΔentA

deletion

deletion

Auxotrophies

Deletion reduces the concentration of cytochrome b1 decreasing iron uptake - Table 2

PMID:4919746[6]


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0588

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCGATTTCAGCGGTAAAAATGT

Primer 2:CCTGCCCCCAGCGTTGAGCCGCC

25A7

Kohara Phage

Genobase

PMID:3038334[8]

purK79::Tn10

Linked marker

CAG12171 = CGSC7342[9]

est. P1 cotransduction: 1% [10]
Synonyms:purE79::Tn10

dsbG601::Tn10

Linked marker

CAG12149 = CGSC7346[9]

est. P1 cotransduction: 77% [10]
Synonyms:zbd-601::Tn10, zbe-601::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10259

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10259

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000253

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945284

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0255

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002054

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Liu, J et al. (1989) Nucleotide sequence of a cluster of Escherichia coli enterobactin biosynthesis genes: identification of entA and purification of its product 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase. J. Bacteriol. 171 791-8 PubMed
  3. Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  5. Ozenberger, BA et al. (1989) Nucleotide sequence of Escherichia coli isochorismate synthetase gene entC and evolutionary relationship of isochorismate synthetase and other chorismate-utilizing enzymes. J. Bacteriol. 171 775-83 PubMed
  6. 6.0 6.1 Cox, GB et al. (1970) Mutations affecting iron transport in Escherichia coli. J. Bacteriol. 104 219-26 PubMed
  7. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. 9.0 9.1 CGSC: The Coli Genetics Stock Center
  10. 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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