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Standard Name	dsbC
Gene Synonym(s)	ECK2888, b2893, JW2861, xprA^[1], xprA
Product Desc.	protein disulfide isomerase II^[1], Component of disulfide oxidoreductase 2^[2] Disulfide bond isomerase, periplasmic; chaperone; activated by DsbD; homodimeric^[3]
Product Synonyms(s)	B2893^[2]^[1], XprA^[2]^[1] , ECK2888, JW2861, xprA, b2893
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): dsbC-recJ-prfB^[2], dsbC^[2]
Regulation/Activity
Quick Links
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Notes

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DsbC rearranges incorrect disulfide bonds during oxidative protein folding.^[3]

Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name	dsbC
Mnemonic	Disulfide bond
Synonyms	ECK2888, b2893, JW2861, xprA^[1], xprA
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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain	Map location	Genome coordinates	Genome browsers	Sequence links
MG1655	65.44 minutes	MG1655: 3036844..3036134 <gbrowseImage> name=NC_000913:3036134..3036844 source=MG1655 preset=GeneLocation </gbrowseImage>	EcoCyc GBrowse(MG1655)	FASTA Dump EcoCyc Sequence Dump
REL606		NC_012967: 2924575..2923865 <gbrowseImage> name=NC_012967:2923865..2924575 source=REL606 preset=GeneLocation </gbrowseImage>	GBrowse (REL606)	FASTA Dump
BW2952		NC_012759: 2923282..2923992 <gbrowseImage> name=NC_012759:2923282..2923992 source=BW2952 preset=GeneLocation </gbrowseImage>	GBrowse (BW2952)	FASTA Dump
W3110		W3110: 3037478..3036768 <gbrowseImage> name=NC_007779:3036768..3037478 source=W3110 preset=GeneLocation </gbrowseImage>	GBrowse(W3110)	FASTA Dump
DH10B		DH10B: 3130714..3130004 <gbrowseImage> name=NC_010473:3130004..3130714 source=DH10B preset=GeneLocation </gbrowseImage>	GBrowse(DH10B)	FASTA Dump
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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type	Strain	Genomic Location	Evidence	Reference	Notes
Coding Start (SO:0000323)	MG1655	3036137	Edman degradation	PMID:9298646
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele	Nt change(s)	AA change(s)	Phenotype: Type	Phenotype: Description	Reference	Availability	Comments
dsbC(del) (Keio:JW2861)	deletion	deletion			PMID:16738554	Shigen CGSC8322^[4]
dsbCC118T,A		C118T,A		Loss of activity			seeded from UniProt:P0AEG6
dsbCC121A		C121A		Partial loss of activity			seeded from UniProt:P0AEG6
dsbCC121V		C121V		Loss of activity			seeded from UniProt:P0AEG6
dsbCC161S		C161S		Destabilization of protein			seeded from UniProt:P0AEG6
dsbCC183L		C183L		Destabilization of protein			seeded from UniProt:P0AEG6
dsbC3::Cm					PMID:8917542 PMID:9352906	CGSC:65870
dsbC744(del)::kan					PMID:16738554	CGSC:100652
dsbC::Tn10	Insertion		Sensitivity to	Dithiothreitol	PMID:8168498		The mutation conferred an increase toward Dithiothreitol
dsbC::Tn10	Insertion		Alkaline phosphate activity	Decreased alkaline phosphatase activity	PMID:8168498		The mutation conferred a reduction of alkaline phosphatase activity.
dsbC::Tn10	Insertion		Sensitivity to	Benzylpenicillin sensitivity	PMID:8168498		The mutation caused hypersensitivity toward Benzylpenicillin
dsbC::Tn10	Insertion		Growth Phenotype	Decreased Growth	PMID:8168498		The mutation caused a reduction of growth on minimal medium.
dsbC13::Tn10	Insertion		Disulfide Bond Formation	Defective Disulfide Bond Formation	PMID:8168498		Defective Disulfide Bond formation. See figure 2 for experimental results.
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Notes

Genetic Interactions

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Interactor	Interaction	Allele	Score(s)	Reference(s)	Accessions	Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource	Resource Type	Source	Notes/Reference
JW2861	Plasmid clone	Shigen	PMID:16769691 Status:Clone OK Primer 1:GCCAAGAAAGGTTTTATGTTGTT Primer 2:CCTTTACCGCTGGTCATTTTTTG
22H4	Kohara Phage	Genobase	PMID:3038334
5E11	Kohara Phage	Genobase	PMID:3038334
recD1901::Tn10	Linked marker	CAG12135 = CGSC7429^[4]	est. P1 cotransduction: % ^[5]
speA210::Tn10	Linked marker	CAG12168 = CGSC7431^[4]	est. P1 cotransduction: 10% ^[5] Synonyms:zgd-210::Tn10, zgf-210::Tn10
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11070	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11070	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001059	Escherichia coli str. K-12 substr. MG1655
NCBI (EcoliWiki Page)	GeneID:947363	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1063	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009494	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	DsbC
Synonyms	B2893^[2]^[1], XprA^[2]^[1] , ECK2888, JW2861, xprA, b2893
Product description	protein disulfide isomerase II^[1], Component of disulfide oxidoreductase 2^[2] Disulfide bond isomerase, periplasmic; chaperone; activated by DsbD; homodimeric^[3]
EC number (for enzymes)	5.3.4.1^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0030288	outer membrane-bounded periplasmic space	PMID:8168498	NAS: Non-traceable Author Statement		C	Authors predicted a signal peptide based on examination of the amino acid sequence. No reference given.	complete
	GO:0003756	protein disulfide isomerase activity	PMID:8168498	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc (v14.0)	complete
	GO:0003756	protein disulfide isomerase activity	PMID:8168498	IMP: Inferred from Mutant Phenotype		F		complete
	GO:0042597	periplasmic space	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0574	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-0200	C	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	PMID:8168498	ISM: Inferred from Sequence Model	PMID:8168498	C		complete
	GO:0045454	cell redox homeostasis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017936	P	Seeded from EcoCyc (v14.0)	complete
	GO:0045454	cell redox homeostasis	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR017937	P	Seeded from EcoCyc (v14.0)	complete
	GO:0042597	periplasmic space	PMID:8168498	IDA: Inferred from Direct Assay		C	Fig 5 and Fig 6	complete
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Interactions

See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of disulfide oxidoreductase 2	could be indirect
Protein	can		PMID:16606699	Experiment(s):EBI-1144206
Protein	slyD		PMID:16606699	Experiment(s):EBI-1144206
Protein	rpsD		PMID:16606699	Experiment(s):EBI-1144206
Protein	nadE		PMID:16606699	Experiment(s):EBI-1144206
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Evidence	Reference/Source
periplasm		From EcoCyc^[6]
Periplasm	PMID:9298646	EchoLocation:dsbC
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MKKGFMLFTL LAAFSGFAQA DDAAIQQTLA KMGIKSSDIQ PAPVAGMKTV LTNSGVLYIT DDGKHIIQGP MYDVSGTAPV NVTNKMLLKQ LNALEKEMIV YKAPQEKHVI TVFTDITCGY CHKLHEQMAD YNALGITVRY LAFPRQGLDS DAEKEMKAIW CAKDKNKAFD DVMAGKSVAP ASCDVDIADH YALGVQLGVS GTPAVVLSNG TLVPGYQPPK EMKEFLDEHQ KMTSGK
Length	236
Mol. Wt	25.621 kDa
pI	6.7 (calculated)
Extinction coefficient	17,420 - 17,920 (calc based on 8 Y, 1 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
motif	1-20	UniProt Manual:Signal Peptides	UniProt:P0AEG6
Domain	25..78	PF10411 Disulfide bond isomerase protein N-terminus		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=dsbC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130795	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:947363	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009494	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P0AEG6	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG11070	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG11070	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:947363	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001059	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1063	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MC4100	1.25E+03	molecules/cell	Medium: median data from rich and minimal media Temperature (°C): 37 Doubling time (min): Growth Rate(min): Growth phase: other:	emPAI		PMID:18304323
Protein	Ecoli K-12	1.016+/-0.052	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		Single Molecule Fluorescence	PMID:20671182
mRNA	Ecoli K-12	0.098591549	Molecules/cell	Medium: Temperature (°C): Doubling time (min): Growth Rate(min): * Growth phase: other: *		by RNA_Seq	PMID:20671182
Protein	E. coli K-12 MG1655	2167	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	706	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	1266	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
dsbC-recJ-prfB
dsbC
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3036824..3036864 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b2893 (EcoliWiki Page)	NCBI GEO profiles for dsbC
microarray	GenExpDB:b2893 (EcoliWiki Page)	Summary of data for dsbC from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to dsbC Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG11070	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1063	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b2893
EcoGene	EcoGene:EG11070	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001059	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0009494	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Shigella flexneri	DSBC	From SHIGELLACYC
E. coli O157	DSBC	From ECOO157CYC
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Notes

Families

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<protect> See Help:Evolution_families for help entering or editing information in this section of EcoliWiki.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF10411 Disulfide bond isomerase protein N-terminus
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 ^1.5 ^1.6 ^1.7 ^1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^4.0 ^4.1 ^4.2 CGSC: The Coli Genetics Stock Center
↑ ^5.0 ^5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

dsbC:On One Page

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Notes

Gene

Nomenclature

Notes

Location(s) and DNA Sequence

Notes

Sequence Features

Notes

Alleles and Phenotypes

Notes

Genetic Interactions

Notes

Genetic Resources

Notes

Accessions in Other Databases

Notes

Links

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to dsbC Expression

Notes

Links

Evolution

Homologs in Other Organisms

Notes

Families

Notes

Links

References

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