dsbC:Gene
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Interactions | Genetic Resources | Accessions | Links | References | Suggestions |
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Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
dsbC |
|---|---|
| Mnemonic |
Disulfide bond |
| Synonyms |
ECK2888, b2893, JW2861, xprA[1], xprA |
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Notes
Location(s) and DNA Sequence
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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
65.44 minutes |
MG1655: 3036844..3036134 |
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NC_012967: 2924575..2923865 |
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NC_012759: 2923282..2923992 |
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W3110 |
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W3110: 3037478..3036768 |
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DH10B: 3130714..3130004 |
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Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
3036137 |
Edman degradation |
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Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
dsbC(del) (Keio:JW2861) |
deletion |
deletion |
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dsbCC118T,A |
C118T,A |
Loss of activity |
seeded from UniProt:P0AEG6 | ||||
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dsbCC121A |
C121A |
Partial loss of activity |
seeded from UniProt:P0AEG6 | ||||
|
dsbCC121V |
C121V |
Loss of activity |
seeded from UniProt:P0AEG6 | ||||
|
dsbCC161S |
C161S |
Destabilization of protein |
seeded from UniProt:P0AEG6 | ||||
|
dsbCC183L |
C183L |
Destabilization of protein |
seeded from UniProt:P0AEG6 | ||||
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dsbC3::Cm |
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dsbC744(del)::kan |
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dsbC::Tn10 |
Insertion |
Sensitivity to |
Dithiothreitol |
The mutation conferred an increase toward Dithiothreitol | |||
|
dsbC::Tn10 |
Insertion |
Alkaline phosphate activity |
Decreased alkaline phosphatase activity |
The mutation conferred a reduction of alkaline phosphatase activity. | |||
|
dsbC::Tn10 |
Insertion |
Sensitivity to |
Benzylpenicillin sensitivity |
The mutation caused hypersensitivity toward Benzylpenicillin | |||
|
dsbC::Tn10 |
Insertion |
Growth Phenotype |
Decreased Growth |
The mutation caused a reduction of growth on minimal medium. | |||
|
dsbC13::Tn10 |
Insertion |
Disulfide Bond Formation |
Defective Disulfide Bond Formation |
Defective Disulfide Bond formation. See figure 2 for experimental results. | |||
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Notes
Genetic Interactions
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| Interactor | Interaction | Allele | Score(s) | Reference(s) | Accessions | Notes |
|---|---|---|---|---|---|---|
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Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2861 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAGAAAGGTTTTATGTTGTT Primer 2:CCTTTACCGCTGGTCATTTTTTG | |
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Kohara Phage |
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Kohara Phage |
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Linked marker |
est. P1 cotransduction: % [10] | ||
|
Linked marker |
est. P1 cotransduction: 10% [10] | ||
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ Rietsch, A et al. (1996) An in vivo pathway for disulfide bond isomerization in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 93 13048-53 PubMed
- ↑ Rietsch, A et al. (1997) Reduction of the periplasmic disulfide bond isomerase, DsbC, occurs by passage of electrons from cytoplasmic thioredoxin. J. Bacteriol. 179 6602-8 PubMed
- ↑ 7.0 7.1 7.2 7.3 7.4 Missiakas, D et al. (1994) The Escherichia coli dsbC (xprA) gene encodes a periplasmic protein involved in disulfide bond formation. EMBO J. 13 2013-20 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 9.0 9.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
- ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
