dnaJ:On One Page

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Quickview | Gene | Product(s) | Expression| Evolution | References |

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Standard Name

dnaJ

Gene Synonym(s)

ECK0015, b0015, JW0014, groP, grpC[1], faa, grpC

Product Desc.

chaperone with DnaK; heat shock protein[2][3];

Component of DnaJ/DnaK/GrpE[2][3]

DnaK co-chaperone; stress-related DNA biosynthesis, responsive to heat shock; binds Zn(II)[4]

Product Synonyms(s)

chaperone Hsp40, co-chaperone with DnaK[1], B0015[2][1], GrpC[2][1], GroP[2][1], DnaJ[2][1] , ECK0015, faa, groP, grpC, JW0014, b0015

Function from GO

<GO_nr />

Knock-Out Phenotype
Regulation/Expression

transcription unit(s): dnaKJ[2]

Regulation/Activity
Quick Links

porteco.png EcoCyc.gif regulondb.jpg

dnadisplay.png proteindisplay.png   pubmed.jpg   textpresso.jpg  

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Notes

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DnaK and DnaJ both bind to the same target peptide, forming a ternary complex.[4]



Gene

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dnaJ

Mnemonic

DNA

Synonyms

ECK0015, b0015, JW0014, groP, grpC[1], faa, grpC

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

0.31 minutes 

MG1655: 14168..15298
<gbrowseImage> name=NC_000913:14168..15298 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 14166..15296
<gbrowseImage> name=NC_012967:14166..15296 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 14168..15298
<gbrowseImage> name=NC_012759:14168..15298 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 14168..15298
<gbrowseImage> name=NC_007779:14168..15298 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 14168..15298
<gbrowseImage> name=NC_010473:14168..15298 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

14171

Edman degradation

PMID:3003084


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

dnaJC144S

C144S

Loss of dnaK-independent chaperone activity; when associated with S-147; S- 197 and S-200

seeded from UniProt:P08622

dnaJC161H

C161H

No effect on chaperone function; when associated with H-183

seeded from UniProt:P08622

dnaJC147S

C147S

Loss of dnaK-independent chaperone activity; when associated with S-144; S- 197 and S-200

seeded from UniProt:P08622

dnaJKE51AA

KE51AA

No effect

seeded from UniProt:P08622

dnaJY54A

Y54A

No effect

seeded from UniProt:P08622

dnaJE55A

E55A

No effect

seeded from UniProt:P08622

dnaJTD58AA

TD58AA

No effect

seeded from UniProt:P08622

dnaJDQ67AA

DQ67AA

No effect

seeded from UniProt:P08622

dnaJSQ60AA

SQ60AA

No effect

seeded from UniProt:P08622

dnaJKR62AA

KR62AA

No effect

seeded from UniProt:P08622

dnaJKE48AA

KE48AA

No effect

seeded from UniProt:P08622

dnaJKE41AA

KE41AA

No effect

seeded from UniProt:P08622

dnaJE44A

E44A

No effect

seeded from UniProt:P08622

dnaJK46A

K46A

No effect

seeded from UniProt:P08622

dnaJF47A

F47A

Loss of function

seeded from UniProt:P08622

dnaJMK30AA

MK30AA

No effect

seeded from UniProt:P08622

dnaJY32A

Y32A

No effect

seeded from UniProt:P08622

dnaJH33Q

H33Q

Loss of ability to stimulate dnaK ATPase activity

seeded from UniProt:P08622

dnaJQ38A

Q38A

No effect

seeded from UniProt:P08622

dnaJP34F

P34F

Loss of function

seeded from UniProt:P08622

dnaJD35N

D35N

Loss of ability to bind dnaK

seeded from UniProt:P08622

dnaJR36A

R36A

Decrease in chaperone function

seeded from UniProt:P08622

dnaJN37A

N37A

Decrease in chaperone function

seeded from UniProt:P08622

dnaJL28A

L28A

No effect

seeded from UniProt:P08622

dnaJRE19AA

RE19AA

No effect

seeded from UniProt:P08622

dnaJY25A

Y25A

Loss of activity

seeded from UniProt:P08622

dnaJK26A

K26A

Loss of activity

seeded from UniProt:P08622

dnaJA29G

A29G

No effect

seeded from UniProt:P08622

dnaJR27A

R27A

No effect

seeded from UniProt:P08622

dnaJ(del) (Keio:JW0014)

deletion

deletion

PMID:16738554

Shigen

CGSC8343[5]

dnaJC161S

C161S

Loss of function; when associated with S-164; S-183 and S-186

seeded from UniProt:P08622

dnaJC164H

C164H

No effect on chaperone function; when associated with H-183

seeded from UniProt:P08622

dnaJC164S

C164S

Loss of function; when associated with S-161; S-183 and S-186

seeded from UniProt:P08622

dnaJC186H

C186H

No effect on chaperone function

seeded from UniProt:P08622

dnaJC186S

C186S

Loss of function; when associated with S-161; S-164 and S-184

seeded from UniProt:P08622

dnaJC197S

C197S

Loss of dnaK-independent chaperone activity; when associated with S-144; S- 147 and S-200

seeded from UniProt:P08622

dnaJC183H

C183H

No effect on chaperone function. Same effect; when associated with H-161 or H-164

seeded from UniProt:P08622

dnaJC183S

C183S

Loss of function; when associated with S-161; S-164 and S-186

seeded from UniProt:P08622

dnaJC200S

C200S

Loss of dnaK-independent chaperone activity; when associated with S-144; S- 147 and S-197

seeded from UniProt:P08622

dnaJ259(ts)

temperature sensitive

CGSC:6712

dnaJ735(del)::kan

PMID:16738554

CGSC:100626

dnaJ(del)

  • defective mini-Tn10 insertion
  • block λ DNA replication at all temp
  • growth defect above 30˚C

PMID:2144273

Strain BW2952

T5C

A2V

Nonsynonomous mutation

PMID:19376874

This is a difference relative to E. coli K-12 MG1655.

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Notes

Genetic Interactions

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<protect>

Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0014

Plasmid clone

Shigen

PMID:16769691

Status:Clone OK

Primer 1:GCCGCTAAGCAAGATTATTACGA

Primer 2:CCGCGGGTCAGGTCGTCAAAAAA

9E4

Kohara Phage

Genobase

PMID:3038334

6H3

Kohara Phage

Genobase

PMID:3038334

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[5]

est. P1 cotransduction: 61% [6]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[5]

est. P1 cotransduction: 52% [6]
Synonyms:car-96::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10240

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10240

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000234

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944753

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0236

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000054

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

DnaJ

Synonyms

chaperone Hsp40, co-chaperone with DnaK[1], B0015[2][1], GrpC[2][1], GroP[2][1], DnaJ[2][1] , ECK0015, faa, groP, grpC, JW0014, b0015

Product description

chaperone with DnaK; heat shock protein[2][3];

Component of DnaJ/DnaK/GrpE[2][3]

DnaK co-chaperone; stress-related DNA biosynthesis, responsive to heat shock; binds Zn(II)[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

GO:0003756

protein disulfide isomerase activity

PMID:7559385

IDA: Inferred from Direct Assay

F

Figure 4A-

complete

GO:0005515

protein binding

PMID:9860950

IMP: Inferred from Mutant Phenotype

F

complete

GO:0008270

zinc ion binding

PMID:12941935

IMP: Inferred from Mutant Phenotype

F

complete

GO:0006457

protein folding

PMID:7559385

IDA: Inferred from Direct Assay

P

complete

GO:0003756

protein disulfide isomerase activity

PMID:7559385

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0042026

protein refolding

PMID:7559385

IDA: Inferred from Direct Assay

P

complete

GO:0005515

protein binding

PMID:10521435

IPI: Inferred from Physical Interaction

UniProtKB:P0A6Y8

F

Seeded from EcoCyc (v14.0)

complete

GO:0051082

unfolded protein binding

PMID:7559385

IDA: Inferred from Direct Assay

F

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012724

F

Seeded from EcoCyc (v14.0)

complete

GO:0042803

protein homodimerization activity

PMID:15849180

IDA: Inferred from Direct Assay

F

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01152

C

Seeded from EcoCyc (v14.0)

complete

GO:0005624

membrane fraction

PMID:6220698

IDA: Inferred from Direct Assay

C

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:6220698

IDA: Inferred from Direct Assay

C

DnaJ can be detected in the soluble fraction as well as being loosely membrane-associated.

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

PMID:11985624

IDA: Inferred from Direct Assay

F

complete

GO:0005737

cytoplasm

PMID:6220698

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0005624

membrane fraction

PMID:3889001

IDA: Inferred from Direct Assay

C

complete

GO:0006260

DNA replication

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0235

P

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

PMID:8662861

IDA: Inferred from Direct Assay

F

complete

GO:0006260

DNA replication

PMID:2144273

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0006260

DNA replication

PMID:2144273

IMP: Inferred from Mutant Phenotype

P

λ DNA replication affected

complete

GO:0006457

protein folding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001305

P

Seeded from EcoCyc (v14.0)

complete

GO:0009408

response to heat

PMID:8349564

IEP: Inferred from Expression Pattern

P

complete

GO:0006457

protein folding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002939

P

Seeded from EcoCyc (v14.0)

complete

GO:0006457

protein folding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003095

P

Seeded from EcoCyc (v14.0)

complete

GO:0006457

protein folding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008971

P

Seeded from EcoCyc (v14.0)

complete

GO:0006457

protein folding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012724

P

Seeded from EcoCyc (v14.0)

complete

GO:0006950

response to stress

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0346

P

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0862

F

Seeded from EcoCyc (v14.0)

complete

GO:0009408

response to heat

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012724

P

Seeded from EcoCyc (v14.0)

complete

GO:0031072

heat shock protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001305

F

Seeded from EcoCyc (v14.0)

complete

GO:0031072

heat shock protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001623

F

Seeded from EcoCyc (v14.0)

complete

GO:0031072

heat shock protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003095

F

Seeded from EcoCyc (v14.0)

complete

GO:0031072

heat shock protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018253

F

Seeded from EcoCyc (v14.0)

complete

GO:0051082

unfolded protein binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01152

F

Seeded from EcoCyc (v14.0)

complete

GO:0051082

unfolded protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001305

F

Seeded from EcoCyc (v14.0)

complete

GO:0051082

unfolded protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002939

F

Seeded from EcoCyc (v14.0)

complete

GO:0051082

unfolded protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003095

F

Seeded from EcoCyc (v14.0)

complete

GO:0051082

unfolded protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR008971

F

Seeded from EcoCyc (v14.0)

complete

GO:0051082

unfolded protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012724

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of DnaJ/DnaK/GrpE

could be indirect

Protein

mnmG

PMID:16606699

Experiment(s):EBI-1135319

Protein

sucB

PMID:16606699

Experiment(s):EBI-1135319

Protein

pdxB

PMID:16606699

Experiment(s):EBI-1135319

Protein

aceE

PMID:15690043

Experiment(s):EBI-883621

Protein

add

PMID:15690043

Experiment(s):EBI-883621

Protein

atpD

PMID:15690043

Experiment(s):EBI-883621

Protein

clpA

PMID:15690043

Experiment(s):EBI-883621

Protein

deaD

PMID:15690043

Experiment(s):EBI-883621, EBI-881486

Protein

dnaK

PMID:15690043

Experiment(s):EBI-883621

Protein

gatY

PMID:15690043

Experiment(s):EBI-883621

Protein

imp

PMID:15690043

Experiment(s):EBI-883621

Protein

infB

PMID:15690043

Experiment(s):EBI-883621

Protein

malT

PMID:15690043

Experiment(s):EBI-883621, EBI-879887

Protein

metK

PMID:15690043

Experiment(s):EBI-883621, EBI-881611, EBI-889076

Protein

mreB

PMID:15690043

Experiment(s):EBI-883621, EBI-888239, EBI-893751

Protein

mukB

PMID:15690043

Experiment(s):EBI-883621

Protein

narG

PMID:15690043

Experiment(s):EBI-883621, EBI-880367

Protein

pstB

PMID:15690043

Experiment(s):EBI-883621, EBI-891381

Protein

recA

PMID:15690043

Experiment(s):EBI-883621

Protein

relE

PMID:15690043

Experiment(s):EBI-883621

Protein

rho

PMID:15690043

Experiment(s):EBI-883621

Protein

rplA

PMID:15690043

Experiment(s):EBI-883621

Protein

rplJ

PMID:15690043

Experiment(s):EBI-883621

Protein

rplS

PMID:15690043

Experiment(s):EBI-883621

Protein

rplV

PMID:15690043

Experiment(s):EBI-883621

Protein

rpoC

PMID:15690043

Experiment(s):EBI-883621

Protein

rpsB

PMID:15690043

Experiment(s):EBI-883621

Protein

rpsE

PMID:15690043

Experiment(s):EBI-883621

Protein

rpsJ

PMID:15690043

Experiment(s):EBI-883621, EBI-890903

Protein

rpsM

PMID:15690043

Experiment(s):EBI-883621

Protein

srmB

PMID:15690043

Experiment(s):EBI-883621, EBI-889906

Protein

trxC

PMID:15690043

Experiment(s):EBI-883621

Protein

tufA

PMID:15690043

Experiment(s):EBI-883621, EBI-894882

Protein

yjbJ

PMID:15690043

Experiment(s):EBI-883621

Protein

rplX

PMID:15690043

Experiment(s):EBI-890903

Protein

rpsF

PMID:15690043

Experiment(s):EBI-890903

Protein

rpsN

PMID:15690043

Experiment(s):EBI-890903

Protein

rplW

PMID:15690043

Experiment(s):EBI-890903

Protein

rpsN

PMID:19402753

LCMS(ID Probability):99.6

Protein

atpD

PMID:19402753

MALDI(Z-score):21.544534

Protein

rpsJ

PMID:19402753

LCMS(ID Probability):99.2 MALDI(Z-score):17.053160

Protein

rpsF

PMID:19402753

LCMS(ID Probability):99.6

Protein

rplX

PMID:19402753

LCMS(ID Probability):99.6

Protein

clpA

PMID:19402753

MALDI(Z-score):25.335090

Protein

rplW

PMID:19402753

LCMS(ID Probability):99.2

Protein

infB

PMID:19402753

MALDI(Z-score):28.138730

Protein

rpsC

PMID:19402753

MALDI(Z-score):21.413861

Protein

fusA

PMID:19402753

MALDI(Z-score):27.235301

Protein

rho

PMID:19402753

MALDI(Z-score):20.842792

Protein

recA

PMID:19402753

MALDI(Z-score):22.079568

Protein

gatY

PMID:19402753

MALDI(Z-score):22.028812

Protein

ompC

PMID:19402753

MALDI(Z-score):18.221442

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MAKQDYYEIL GVSKTAEERE IRKAYKRLAM KYHPDRNQGD KEAEAKFKEI KEAYEVLTDS
QKRAAYDQYG HAAFEQGGMG GGGFGGGADF SDIFGDVFGD IFGGGRGRQR AARGADLRYN
MELTLEEAVR GVTKEIRIPT LEECDVCHGS GAKPGTQPQT CPTCHGSGQV QMRQGFFAVQ
QTCPHCQGRG TLIKDPCNKC HGHGRVERSK TLSVKIPAGV DTGDRIRLAG EGEAGEHGAP
AGDLYVQVQV KQHPIFEREG NNLYCEVPIN FAMAALGGEI EVPTLDGRVK LKVPGETQTG
KLFRMRGKGV KSVRGGAQGD LLCRVVVETP VGLNERQKQL LQELQESFGG PTGEHNSPRS
KSFFDGVKKF FDDLTR
Length

376

Mol. Wt

41.099 kDa

pI

8.0 (calculated)

Extinction coefficient

14,900 - 16,150 (calc based on 10 Y, 0 W, and 10 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P08622

Domain

5..67

PF00226 DnaJ domain

PMID:19920124

Domain

242..340

PF01556 DnaJ C terminal region

PMID:19920124

Domain

131..204

PF00684 DnaJ central domain (4 repeats)

PMID:19920124

Motif

zinc finger

PMID:8662861


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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=dnaJ taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128009

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000054

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P08622

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10240

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10240

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944753

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000234

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944753

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0236

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

8.05E+02

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323

Protein

Ecoli K-12

68.948+/-0.33

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182

mRNA

Ecoli K-12

0.12699115

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182

Protein

E. coli K-12 MG1655

5688

molecules/cell/generation

  • Medium: MOPS Complete
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

938

molecules/cell/generation

  • Medium: MOPS Minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

Protein

E. coli K-12 MG1655

4951

molecules/cell/generation

  • Medium: MOPS Complete without Methionine
  • Temperature (°C): 37
  • Doubling time (min):
  • *Growth phase:
  • OD:
  • other:

Ribosome Profiling

PMID: 24766808

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Notes

Transcription and Transcriptional Regulation

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<protect>

See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

dnaKJ

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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:14148..14188 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b0015 (EcoliWiki Page)

NCBI GEO profiles for dnaJ

microarray

GenExpDB:b0015 (EcoliWiki Page)

Summary of data for dnaJ from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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Notes

Accessions Related to dnaJ Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10240

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0236

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0015

EcoGene

EcoGene:EG10240

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000234

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000054

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism Homologs (Statistics) Comments

Anopheles gambiae

  • ENSANGP00000010875 (score: 1.000; bootstrap: 68%)

From Inparanoid:20070104

Apis mellifera

  • ENSAPMP00000002749 (score: 1.000; bootstrap: 97%)

From Inparanoid:20070104

Arabidopsis thaliana

  • AT2G22360 (score: 1.000; bootstrap: 63%)
  • AT4G39960 (score: 0.769)
  • AT1G80030 (score: 0.169)
  • AT3G17830 (score: 0.084)

From Inparanoid:20070104

Bos taurus

  • ENSBTAP00000013207 (score: 1.000; bootstrap: 61%)

From Inparanoid:20070104

Caenorhabditis briggsae

  • WBGene00041373 (score: 1.000; bootstrap: 93%)

From Inparanoid:20070104

Caenorhabditis elegans

  • WBGene00001028 (score: 1.000; bootstrap: 94%)

From Inparanoid:20070104

Canis familiaris

  • ENSCAFP00000028338 (score: 1.000; bootstrap: 86%)

From Inparanoid:20070104

Ciona intestinalis

  • ENSCINP00000014962 (score: 1.000; bootstrap: 85%)

From Inparanoid:20070104

Danio rerio

  • ZDB-CDNA-040425-242 (score: 1.000; bootstrap: 90%)
  • ZDB-GENE-030131-7837 (score: 1.000; bootstrap: 91%)
  • ZDB-CDNA-040425-243 (score: 1.000; bootstrap: 91%)
  • ZDB-CDNA-040425-244 (score: 0.502)
  • ZDB-GENE-040115-3 (score: 0.502)

From Inparanoid:20070104

Dictyostelium discoideum

  • DDB0206193 (score: 1.000; bootstrap: 99%)

From Inparanoid:20070104

Drosophila melanogaster

  • FBgn0002174 (score: 1.000; bootstrap: 75%)

From Inparanoid:20070104

Drosophila pseudoobscura

  • GA21376-PA (score: 1.000; bootstrap: 84%)
  • GA22062-PA (score: 0.069)

From Inparanoid:20070104

Gallus gallus

  • ENSGALP00000012682 (score: 1.000; bootstrap: 96%)

From Inparanoid:20070104

Homo sapiens

  • ENSP00000262375 (score: 1.000; bootstrap: 87%)

From Inparanoid:20070104

Macaca mulatta

  • ENSMMUP00000010650 (score: 1.000; bootstrap: 53%)

From Inparanoid:20070104

Monodelphis domestica

  • ENSMODP00000020443 (score: 1.000; bootstrap: 86%)

From Inparanoid:20070104

Mus musculus

  • MGI:1933786 (score: 1.000; bootstrap: 87%)

From Inparanoid:20070104

Oryza gramene

  • Q5W730 (score: 1.000; bootstrap: 56%)
  • Q948S9 (score: 0.958)
  • Q6K850 (score: 0.173)

From Inparanoid:20070104

Pan troglodytes

  • ENSPTRP00000044217 (score: 1.000; bootstrap: 67%)

From Inparanoid:20070104

Rattus norvegicus

  • ENSRNOP00000005479 (score: 1.000; bootstrap: 88%)

From Inparanoid:20070104

Saccharomyces cerevisiae

  • YFL016C (score: 1.000; bootstrap: 65%)

From Inparanoid:20070104

Schizosaccharomyces pombe

  • SPCC4G34 (score: 1.000; bootstrap: 83%)

From Inparanoid:20070104

Takifugu rubripes

  • NEWSINFRUP00000147424 (score: 1.000; bootstrap: 85%)
  • NEWSINFRUP00000151350 (score: 0.309)

From Inparanoid:20070104

Tetraodon nigroviridis

  • GSTENP00018520001 (score: 1.000; bootstrap: 96%)
  • GSTENP00004136001 (score: 0.290)
  • GSTENP00002750001 (score: 0.051)

From Inparanoid:20070104

Xenopus tropicalis

  • ENSXETP00000037678 (score: 1.000; bootstrap: 81%)

From Inparanoid:20070104

Shigella flexneri

DNAJ

From SHIGELLACYC

E. coli O157

DNAJ

From ECOO157CYC

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Do-It-Yourself Web Tools

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Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

Pfam (EcoliWiki Page)

PF01556 DnaJ C terminal region

Pfam (EcoliWiki Page)

PF00226 DnaJ domain

Pfam (EcoliWiki Page)

PF00684 DnaJ central domain (4 repeats)

Superfamily (EcoliWiki Page)

SUPERFAMILY:46565

Superfamily (EcoliWiki Page)

SUPERFAMILY:49493

Superfamily (EcoliWiki Page)

SUPERFAMILY:49493

Superfamily (EcoliWiki Page)

SUPERFAMILY:57938

EcoCyc

EcoCyc:EG10240

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10240

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000234

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0236

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000054

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ 4.0 4.1 4.2 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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