dnaJ:Gene Product(s)
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
DnaJ |
|---|---|
| Synonyms |
chaperone Hsp40, co-chaperone with DnaK[1], B0015[2][1], GrpC[2][1], GroP[2][1], DnaJ[2][1] , ECK0015, faa, groP, grpC, JW0014, b0015 |
| Product description |
chaperone with DnaK; heat shock protein[2][3]; Component of DnaJ/DnaK/GrpE[2][3] DnaK co-chaperone; stress-related DNA biosynthesis, responsive to heat shock; binds Zn(II)[4] |
| EC number (for enzymes) |
|
| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0003756 |
protein disulfide isomerase activity |
IDA: Inferred from Direct Assay |
F |
Figure 4A- |
complete | |||
| GO:0005515 |
protein binding |
IMP: Inferred from Mutant Phenotype |
F |
complete | ||||
| GO:0008270 |
zinc ion binding |
IMP: Inferred from Mutant Phenotype |
F |
complete | ||||
| GO:0006457 |
protein folding |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0003756 |
protein disulfide isomerase activity |
IDA: Inferred from Direct Assay |
F |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0042026 |
protein refolding |
IDA: Inferred from Direct Assay |
P |
complete | ||||
| GO:0005515 |
protein binding |
IPI: Inferred from Physical Interaction |
F |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0051082 |
unfolded protein binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0005524 |
ATP binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0042803 |
protein homodimerization activity |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0005737 |
cytoplasm |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005624 |
membrane fraction |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0005737 |
cytoplasm |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
C |
DnaJ can be detected in the soluble fraction as well as being loosely membrane-associated. |
complete | |||
| GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0086 |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008270 |
zinc ion binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
C |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0005624 |
membrane fraction |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0006260 |
DNA replication |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008270 |
zinc ion binding |
IDA: Inferred from Direct Assay |
F |
complete | ||||
| GO:0006260 |
DNA replication |
IMP: Inferred from Mutant Phenotype |
P |
Seeded from EcoCyc (v14.0) |
complete | |||
| GO:0006260 |
DNA replication |
IMP: Inferred from Mutant Phenotype |
P |
λ DNA replication affected |
complete | |||
| GO:0006457 |
protein folding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009408 |
response to heat |
IEP: Inferred from Expression Pattern |
P |
complete | ||||
| GO:0006457 |
protein folding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006457 |
protein folding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006457 |
protein folding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006457 |
protein folding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006950 |
response to stress |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0008270 |
zinc ion binding |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0009408 |
response to heat |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0031072 |
heat shock protein binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0031072 |
heat shock protein binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0031072 |
heat shock protein binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0031072 |
heat shock protein binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0051082 |
unfolded protein binding |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0051082 |
unfolded protein binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0051082 |
unfolded protein binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0051082 |
unfolded protein binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0051082 |
unfolded protein binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0051082 |
unfolded protein binding |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| edit table |
Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of DnaJ/DnaK/GrpE |
could be indirect |
||
|
Protein |
mnmG |
Experiment(s):EBI-1135319 | ||
|
Protein |
sucB |
Experiment(s):EBI-1135319 | ||
|
Protein |
pdxB |
Experiment(s):EBI-1135319 | ||
|
Protein |
aceE |
Experiment(s):EBI-883621 | ||
|
Protein |
add |
Experiment(s):EBI-883621 | ||
|
Protein |
atpD |
Experiment(s):EBI-883621 | ||
|
Protein |
clpA |
Experiment(s):EBI-883621 | ||
|
Protein |
deaD |
Experiment(s):EBI-883621, EBI-881486 | ||
|
Protein |
dnaK |
Experiment(s):EBI-883621 | ||
|
Protein |
gatY |
Experiment(s):EBI-883621 | ||
|
Protein |
imp |
Experiment(s):EBI-883621 | ||
|
Protein |
infB |
Experiment(s):EBI-883621 | ||
|
Protein |
malT |
Experiment(s):EBI-883621, EBI-879887 | ||
|
Protein |
metK |
Experiment(s):EBI-883621, EBI-881611, EBI-889076 | ||
|
Protein |
mreB |
Experiment(s):EBI-883621, EBI-888239, EBI-893751 | ||
|
Protein |
mukB |
Experiment(s):EBI-883621 | ||
|
Protein |
narG |
Experiment(s):EBI-883621, EBI-880367 | ||
|
Protein |
pstB |
Experiment(s):EBI-883621, EBI-891381 | ||
|
Protein |
recA |
Experiment(s):EBI-883621 | ||
|
Protein |
relE |
Experiment(s):EBI-883621 | ||
|
Protein |
rho |
Experiment(s):EBI-883621 | ||
|
Protein |
rplA |
Experiment(s):EBI-883621 | ||
|
Protein |
rplJ |
Experiment(s):EBI-883621 | ||
|
Protein |
rplS |
Experiment(s):EBI-883621 | ||
|
Protein |
rplV |
Experiment(s):EBI-883621 | ||
|
Protein |
rpoC |
Experiment(s):EBI-883621 | ||
|
Protein |
rpsB |
Experiment(s):EBI-883621 | ||
|
Protein |
rpsE |
Experiment(s):EBI-883621 | ||
|
Protein |
rpsJ |
Experiment(s):EBI-883621, EBI-890903 | ||
|
Protein |
rpsM |
Experiment(s):EBI-883621 | ||
|
Protein |
srmB |
Experiment(s):EBI-883621, EBI-889906 | ||
|
Protein |
trxC |
Experiment(s):EBI-883621 | ||
|
Protein |
tufA |
Experiment(s):EBI-883621, EBI-894882 | ||
|
Protein |
yjbJ |
Experiment(s):EBI-883621 | ||
|
Protein |
rplX |
Experiment(s):EBI-890903 | ||
|
Protein |
rpsF |
Experiment(s):EBI-890903 | ||
|
Protein |
rpsN |
Experiment(s):EBI-890903 | ||
|
Protein |
rplW |
Experiment(s):EBI-890903 | ||
|
Protein |
rpsN |
LCMS(ID Probability):99.6 | ||
|
Protein |
atpD |
MALDI(Z-score):21.544534 | ||
|
Protein |
rpsJ |
LCMS(ID Probability):99.2 MALDI(Z-score):17.053160 | ||
|
Protein |
rpsF |
LCMS(ID Probability):99.6 | ||
|
Protein |
rplX |
LCMS(ID Probability):99.6 | ||
|
Protein |
clpA |
MALDI(Z-score):25.335090 | ||
|
Protein |
rplW |
LCMS(ID Probability):99.2 | ||
|
Protein |
infB |
MALDI(Z-score):28.138730 | ||
|
Protein |
rpsC |
MALDI(Z-score):21.413861 | ||
|
Protein |
fusA |
MALDI(Z-score):27.235301 | ||
|
Protein |
rho |
MALDI(Z-score):20.842792 | ||
|
Protein |
recA |
MALDI(Z-score):22.079568 | ||
|
Protein |
gatY |
MALDI(Z-score):22.028812 | ||
|
Protein |
ompC |
MALDI(Z-score):18.221442 | ||
| edit table |
</protect>
Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
| edit table |
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Notes
Structure and Physical Properties
<protect>
Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MAKQDYYEIL GVSKTAEERE IRKAYKRLAM KYHPDRNQGD KEAEAKFKEI KEAYEVLTDS QKRAAYDQYG HAAFEQGGMG GGGFGGGADF SDIFGDVFGD IFGGGRGRQR AARGADLRYN MELTLEEAVR GVTKEIRIPT LEECDVCHGS GAKPGTQPQT CPTCHGSGQV QMRQGFFAVQ QTCPHCQGRG TLIKDPCNKC HGHGRVERSK TLSVKIPAGV DTGDRIRLAG EGEAGEHGAP AGDLYVQVQV KQHPIFEREG NNLYCEVPIN FAMAALGGEI EVPTLDGRVK LKVPGETQTG KLFRMRGKGV KSVRGGAQGD LLCRVVVETP VGLNERQKQL LQELQESFGG PTGEHNSPRS KSFFDGVKKF FDDLTR |
| Length |
376 |
| Mol. Wt |
41.099 kDa |
| pI |
8.0 (calculated) |
| Extinction coefficient |
14,900 - 16,150 (calc based on 10 Y, 0 W, and 10 C residues) |
| edit table |
Domains/Motifs/Modification Sites
|
See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.
|
<motif_map/> |
|
Structure
| </protect>
Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
| edit table |
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
| edit table |
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Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 5.3 5.4 de Crouy-Chanel, A et al. (1995) A novel function of Escherichia coli chaperone DnaJ. Protein-disulfide isomerase. J. Biol. Chem. 270 22669-72 PubMed
- ↑ Suh, WC et al. (1998) Interaction of the Hsp70 molecular chaperone, DnaK, with its cochaperone DnaJ. Proc. Natl. Acad. Sci. U.S.A. 95 15223-8 PubMed
- ↑ Linke, K et al. (2003) The roles of the two zinc binding sites in DnaJ. J. Biol. Chem. 278 44457-66 PubMed
- ↑ Suh, WC et al. (1999) Structural features required for the interaction of the Hsp70 molecular chaperone DnaK with its cochaperone DnaJ. J. Biol. Chem. 274 30534-9 PubMed
- ↑ Shi, YY et al. (2005) The C-terminal (331-376) sequence of Escherichia coli DnaJ is essential for dimerization and chaperone activity: a small angle X-ray scattering study in solution. J. Biol. Chem. 280 22761-8 PubMed
- ↑ 10.0 10.1 10.2 Zylicz, M et al. (1983) Escherichia coli dnaJ- and dnaK-gene products: synthesis in minicells and membrane-affinity. Biochem. Biophys. Res. Commun. 110 176-80 PubMed
- ↑ Katayama, A et al. (2002) Systematic search for zinc-binding proteins in Escherichia coli. Eur. J. Biochem. 269 2403-13 PubMed
- ↑ Zylicz, M et al. (1985) Purification and properties of the dnaJ replication protein of Escherichia coli. J. Biol. Chem. 260 7591-8 PubMed
- ↑ 13.0 13.1 Banecki, B et al. (1996) Structure-function analysis of the zinc finger region of the DnaJ molecular chaperone. J. Biol. Chem. 271 14840-8 PubMed
- ↑ 14.0 14.1 Sell, SM et al. (1990) Isolation and characterization of dnaJ null mutants of Escherichia coli. J. Bacteriol. 172 4827-35 PubMed
- ↑ Chuang, SE & Blattner, FR (1993) Characterization of twenty-six new heat shock genes of Escherichia coli. J. Bacteriol. 175 5242-52 PubMed
- ↑ 16.0 16.1 16.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
- ↑ 17.00 17.01 17.02 17.03 17.04 17.05 17.06 17.07 17.08 17.09 17.10 17.11 17.12 17.13 17.14 17.15 17.16 17.17 17.18 17.19 17.20 17.21 17.22 17.23 17.24 17.25 17.26 17.27 17.28 17.29 17.30 17.31 17.32 17.33 17.34 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
- ↑ 18.00 18.01 18.02 18.03 18.04 18.05 18.06 18.07 18.08 18.09 18.10 18.11 18.12 18.13 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
- ↑ 19.0 19.1 19.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0003756 ! protein disulfide isomerase activity
- GO:0005515 ! protein binding
- GO:0008270 ! zinc ion binding
- GO:0006457 ! protein folding
- GO:0042026 ! protein refolding
- GO:0051082 ! unfolded protein binding
- GO:0005524 ! ATP binding
- GO:0042803 ! protein homodimerization activity
- GO:0005737 ! cytoplasm
- GO:0005624 ! obsolete membrane fraction
- GO:0006260 ! DNA replication
- GO:0009408 ! response to heat
- GO:0006950 ! response to stress
- GO:0031072 ! heat shock protein binding
- Proteins
- RefGenome Annotated Gene
