dadX:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dadX

Mnemonic

D-Amino acid dehydrogenase

Synonyms

ECK1178, b1190, JW1179, alnA, alnB, dadB, msuA?[1][2], msuA?

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

26.69 minutes 

MG1655: 1238102..1239172
<gbrowseImage> name=NC_000913:1238102..1239172 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1239029..1240099
<gbrowseImage> name=NC_012967:1239029..1240099 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1126823..1127893
<gbrowseImage> name=NC_012759:1126823..1127893 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1240456..1241526
<gbrowseImage> name=NC_007779:1240456..1241526 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1278343..1279413
<gbrowseImage> name=NC_010473:1278343..1279413 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔdadX (Keio:JW1179)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC9085[4]

dadX::Tn5KAN-2 (FB20266)

Insertion at nt 307 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB20266

does not contain pKD46

dadB3

PMID:4571807[6]

CGSC:6496

dadX20

PMID:4594607[7] PMID:6298185[8]

CGSC:79525

dadB21(ts)

temperature sensitive

CGSC:79526

ΔdadX779::kan

PMID:16738554[3]

CGSC:100526


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1179

Plasmid clone

Shigen

PMID:16769691[9]

Status:Clone OK

Primer 1:GCCACCCGTCCGATACAGGCCAG

Primer 2:CCaACCGTCACAACCGGGACGCG

11G8

Kohara Phage

Genobase

PMID:3038334[10]

7C10

Kohara Phage

Genobase

PMID:3038334[10]

fadR13::Tn10

Linked marker

CAG18497 = CGSC7365[4]

est. P1 cotransduction: 88% [11]

oppC506::Tn10

Linked marker

CAG12169 = CGSC7369[4]

est. P1 cotransduction: 3% [11]
Synonyms:zch-506::Tn10, zci-506::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11408

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11408

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001374

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945754

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1380

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003997

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. Beelen, RH et al. (1973) A regulatory gene and a structural gene for alaninase in Escherichia coli. Mol. Gen. Genet. 121 369-74 PubMed
  7. Wijsman, HJ (1972) The characterization of an alanine racemase mutant of Escherichia coli. Genet. Res. 20 269-77 PubMed
  8. Wasserman, SA et al. (1983) Two alanine racemase genes in Salmonella typhimurium that differ in structure and function. J. Bacteriol. 153 1439-50 PubMed
  9. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  10. 10.0 10.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  11. 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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