PMID:6298185
| Citation |
Wasserman, SA, Walsh, CT and Botstein, D (1983) Two alanine racemase genes in Salmonella typhimurium that differ in structure and function. J. Bacteriol. 153:1439-50 |
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| Abstract |
Mutations were isolated in a previously undescribed Salmonella typhimurium gene encoding an alanine racemase essential for utilization of L-alanine as a source of carbon, energy, and nitrogen. This new locus, designated dadB, lies within one kilobase of the D-alanine dehydrogenase locus (dadA), which is also required for alanine catabolism. The dadA and dadB genes are coregulated. Mutants (including insertions) lacking the dadB alanine racemase do not require D-alanine for growth unless a mutation is introduced at a second locus, designated dal. Two genes specifying alanine racemase activity were cloned from S. typhimurium. The two cloned DNA sequences do not cross-hybridize with each other; one was shown to contain the dadB gene. |
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| Keywords |
Alanine Racemase/genetics; Amino Acid Isomerases/genetics; Base Sequence; DNA Restriction Enzymes/metabolism; DNA, Bacterial/analysis; Genes, Bacterial; Salmonella typhimurium/enzymology; Salmonella typhimurium/genetics |
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